##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064108_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2523584
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.287570376100025	32.0	32.0	32.0	32.0	32.0
2	30.92103333988486	32.0	32.0	32.0	32.0	32.0
3	30.94290857764196	32.0	32.0	32.0	32.0	32.0
4	30.973962428038853	32.0	32.0	32.0	32.0	32.0
5	30.88759320078111	32.0	32.0	32.0	32.0	32.0
6	34.54840100428597	36.0	36.0	36.0	32.0	36.0
7	34.49446461857422	36.0	36.0	36.0	32.0	36.0
8	34.454576507062974	36.0	36.0	36.0	32.0	36.0
9	34.58680313395552	36.0	36.0	36.0	32.0	36.0
10	34.30002805533717	36.0	36.0	36.0	32.0	36.0
11	34.54355155207831	36.0	36.0	36.0	32.0	36.0
12	34.37522507671629	36.0	36.0	36.0	32.0	36.0
13	34.45666401435419	36.0	36.0	36.0	32.0	36.0
14	34.3662719370546	36.0	36.0	36.0	32.0	36.0
15	34.303537350054526	36.0	36.0	36.0	32.0	36.0
16	34.30184174570769	36.0	36.0	36.0	32.0	36.0
17	34.22560374451574	36.0	36.0	36.0	32.0	36.0
18	34.22766589105019	36.0	36.0	36.0	32.0	36.0
19	34.23204299916309	36.0	36.0	36.0	32.0	36.0
20	34.20728614541858	36.0	36.0	36.0	32.0	36.0
21	34.185584470340594	36.0	36.0	36.0	32.0	36.0
22	34.169730827267884	36.0	36.0	36.0	32.0	36.0
23	34.12399309870406	36.0	36.0	36.0	32.0	36.0
24	34.09413675154067	36.0	36.0	36.0	32.0	36.0
25	33.70528423068144	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	5.0
4	19.0
5	23.0
6	98.0
7	38.0
8	137.0
9	165.0
10	219.0
11	72.0
12	150.0
13	132.0
14	239.0
15	405.0
16	663.0
17	903.0
18	1240.0
19	1640.0
20	2484.0
21	3477.0
22	5232.0
23	7947.0
24	11839.0
25	17721.0
26	25587.0
27	34263.0
28	47105.0
29	64219.0
30	85882.0
31	117398.0
32	166428.0
33	238571.0
34	519329.0
35	1169953.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.232339649567905	16.676444937762625	10.981289146119083	26.10992626655039
2	16.774825298975134	19.164644966779658	37.89052623903586	26.170003495209343
3	19.29631641787023	23.130638636301494	28.350232153209625	29.22281279261865
4	12.415449805646995	15.113090900300813	35.67337599628453	36.79808329776766
5	14.55194010544637	36.55507242701025	33.65010573170461	15.242881735838768
6	34.92550575005093	35.42521208489578	16.482422495781286	13.166859669272005
7	30.612502120467944	30.037082426354367	20.32422945761997	19.026185995557718
8	28.223062674093054	33.613639579865676	18.755809708342237	19.407488037699036
9	27.50841470521771	13.583613823507257	17.98740866724549	40.920562804029544
10	15.826895719376596	26.7379971738465	31.44860984015263	25.98649726662427
11	38.195702698480794	20.283486781955993	22.355657722479357	19.165152797083852
12	25.10961408072069	23.038227142420986	29.082841996328355	22.76931678052997
13	30.24003559078684	19.161055051128052	24.982702227318704	25.616207130766405
14	23.57590758159848	19.625944022059365	24.78498541985042	32.013162976491735
15	25.181983233633602	27.823540397125697	21.85590980118339	25.138566568057307
16	25.728723556113852	25.351831761529997	24.26850080731372	24.650943875042426
17	24.029600000317206	25.70828940372169	25.235772654297516	25.02633794166359
18	24.782839739217337	24.739138021287733	26.61438587585857	23.863636363636363
19	26.07702889565056	24.252209495945042	24.80742379534042	24.863337813063975
20	26.067369210861212	24.320936295530174	24.561894704030816	25.049799789577797
21	27.19691703344573	23.91059777684103	23.90540292426579	24.987082265447448
22	26.27579033110687	24.36793934724046	24.575939480487445	24.78033084116523
23	24.676035961148507	24.193267576152124	25.272427234752026	25.858269227947346
24	25.20537221594985	24.683807092286333	25.11519284465949	24.995627847104327
25	25.196788878221106	24.414788799699874	25.178387943263253	25.210034378815767
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	152.0
1	152.0
2	379.0
3	606.0
4	606.0
5	606.0
6	1760.0
7	2914.0
8	2914.0
9	2914.0
10	3221.5
11	3529.0
12	3529.0
13	3529.0
14	4411.5
15	5294.0
16	5294.0
17	5294.0
18	9050.0
19	12806.0
20	12806.0
21	12806.0
22	22473.0
23	32140.0
24	32140.0
25	32140.0
26	50618.0
27	69096.0
28	69096.0
29	69096.0
30	91639.0
31	114182.0
32	114182.0
33	114182.0
34	136498.5
35	158815.0
36	158815.0
37	158815.0
38	179442.0
39	200069.0
40	200069.0
41	200069.0
42	229828.0
43	259587.0
44	259587.0
45	259587.0
46	290023.0
47	320459.0
48	320459.0
49	320459.0
50	328273.5
51	336088.0
52	336088.0
53	336088.0
54	314397.0
55	292706.0
56	292706.0
57	292706.0
58	271816.5
59	250927.0
60	250927.0
61	250927.0
62	223680.5
63	196434.0
64	196434.0
65	196434.0
66	162427.5
67	128421.0
68	128421.0
69	128421.0
70	97056.5
71	65692.0
72	65692.0
73	65692.0
74	50856.0
75	36020.0
76	36020.0
77	36020.0
78	28814.0
79	21608.0
80	21608.0
81	21608.0
82	14740.0
83	7872.0
84	7872.0
85	7872.0
86	6096.5
87	4321.0
88	4321.0
89	4321.0
90	3069.5
91	1818.0
92	1818.0
93	1818.0
94	1244.5
95	671.0
96	671.0
97	671.0
98	1014.0
99	1357.0
100	1357.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03899216352615962
2	0.0051514037178869414
3	0.0013869163855849459
4	0.003288973142958586
5	0.00855925540818138
6	0.011967107098475819
7	0.022032157439577996
8	0.03435590018006138
9	0.048106185488574975
10	0.05658618853186579
11	0.05349534630113362
12	0.06157908751997159
13	0.06272824681088485
14	0.06585871522406228
15	0.05987516167482437
16	0.06498693921026603
17	0.061697966067307444
18	0.07703329867363243
19	0.07350656843600213
20	0.07873722451877964
21	0.0735858208008927
22	0.0773106819507494
23	0.08262059039841749
24	0.07699367249118713
25	0.07818245796454566
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2523584.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.70399163574685
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.96674128124992	28.761884015379636
2	15.796082625588854	13.175193953902472
3	6.2323094958227445	7.797365492555322
4	3.0187707661144736	5.035791631211005
5	1.6926910442300225	3.5295986575236227
6	1.0283595406401334	2.573201860883796
7	0.6885386056485278	2.0100365775598497
8	0.44592737419525597	1.4877561186871608
9	0.3273986200463193	1.2288446380770084
>10	1.5670205252688558	11.519266752279272
>50	0.11561031714607593	3.386202162421361
>100	0.09610513706605835	8.546110348864978
>500	0.01508694290341351	4.388570541054284
>1k	0.009262237098930886	6.34358603166277
>5k	9.548698040134935E-5	0.21659121793742467
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	5439	0.2155268063199006	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3736	0.14804341761558165	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	3161	0.12525836270954324	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	2859	0.11329125561106743	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	2815	0.11154770358347493	No Hit
GAATAGGACCGCGGTTCTATTTTGT	2765	0.10956639446121072	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	2706	0.10722844969693897	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	2674	0.10596041185868986	No Hit
GTATCTGATCGTCTTCGAACCTCCG	2592	0.10271106489817657	No Hit
CTATTGGAGCTGGAATTACCGCGGC	2580	0.10223555070883315	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.37757094671705E-4
2	0.0	0.0	0.0	0.0	2.37757094671705E-4
3	0.0	0.0	0.0	0.0	2.37757094671705E-4
4	0.0	0.0	0.0	0.0	2.37757094671705E-4
5	0.0	0.0	0.0	0.0	2.37757094671705E-4
6	0.0	0.0	0.0	0.0	2.7738327711698916E-4
7	0.0	0.0	0.0	3.9626182445284164E-5	2.7738327711698916E-4
8	0.0	0.0	0.0	3.9626182445284164E-5	2.7738327711698916E-4
9	0.0	0.0	0.0	3.9626182445284164E-5	2.7738327711698916E-4
10	0.0	0.0	0.0	3.9626182445284164E-5	2.7738327711698916E-4
11	0.0	0.0	0.0	7.925236489056833E-5	3.170094595622733E-4
12	0.0	0.0	0.0	7.925236489056833E-5	0.0010699069260226724
13	0.0	0.0	0.0	7.925236489056833E-5	0.0012284116558038093
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCTTA	40	0.005271833	14.253451	17
ACTAGAC	100	1.0140866E-8	13.294519	3
CGCATCG	370	0.0	12.325353	13
AGTCGAG	55	0.0030678944	12.091679	10
CGGAATT	150	1.4551915E-11	12.036009	15
ATCGCCA	390	0.0	11.938551	16
GTCCTAC	1365	0.0	11.898082	1
ACTCTAA	445	0.0	11.742321	10
CGAACGA	230	0.0	11.567788	16
TCGCGTA	215	0.0	11.488647	9
GCGTAAC	215	0.0	11.488192	11
GCGTTAT	215	0.0	11.485458	1
TCCAACG	390	0.0	11.451944	18
TCCTACA	1530	0.0	11.419676	2
GTATTAA	275	0.0	11.39711	1
CGCCAGT	410	0.0	11.356859	18
AGGCCCG	645	0.0	11.341807	10
CCAACGA	310	0.0	11.341681	19
TTCGTTC	260	0.0	11.330116	18
TTTCGTT	260	0.0	11.329666	17
>>END_MODULE