FastQCFastQC Report
Thu 2 Feb 2017
SRR4064108_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064108_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2523584
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA29010.11495555527376937No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC28000.11095331084679566No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT27400.10857573990007861No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT26880.10651517841292384No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA26740.10596041185868986No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC26520.10508863584489361No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA40900.023.7746871
TCTATAC2100.020.950393
TACGATA550.00448740719.99812
TAGGACG12900.019.7810674
GATTTCG6050.019.27280641
GATATAC11450.019.213671
GCGAGAA9600.018.56294420
AGGACGT14150.018.5000085
GTAGGAC16450.018.4541723
ATTTCGT6100.018.39315442
TTAGGAC9450.018.3897883
TGGCGAG16900.018.3536518
GTCCTAC16350.018.2994041
TATGGCG16150.018.11592716
GACGTGA7900.018.0995467
TGTAGGA17100.018.0099852
TCCTACA17800.017.9196452
GTCCTAA8800.017.7497221
ATACGAA8600.017.65088540
GGTATCA21600.017.6200921
Produced by FastQC (version 0.11.5)