##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064108_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2523584
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.323555704902233	32.0	32.0	32.0	32.0	32.0
2	31.406857469376884	32.0	32.0	32.0	32.0	32.0
3	31.478886377469504	32.0	32.0	32.0	32.0	32.0
4	31.56799615150516	32.0	32.0	32.0	32.0	32.0
5	31.507334806370622	32.0	32.0	32.0	32.0	32.0
6	35.10583875947858	36.0	36.0	36.0	36.0	36.0
7	35.1266837164921	36.0	36.0	36.0	36.0	36.0
8	35.06825808057113	36.0	36.0	36.0	36.0	36.0
9	35.17817041160508	36.0	36.0	36.0	36.0	36.0
10	35.02769830526743	36.0	36.0	36.0	36.0	36.0
11	35.17453153927113	36.0	36.0	36.0	36.0	36.0
12	35.075062688620626	36.0	36.0	36.0	36.0	36.0
13	35.12721114098045	36.0	36.0	36.0	36.0	36.0
14	35.06432161560701	36.0	36.0	36.0	36.0	36.0
15	35.03329589979965	36.0	36.0	36.0	36.0	36.0
16	35.048546432375545	36.0	36.0	36.0	36.0	36.0
17	35.01331875618168	36.0	36.0	36.0	36.0	36.0
18	35.013473694555046	36.0	36.0	36.0	36.0	36.0
19	35.00623478354594	36.0	36.0	36.0	36.0	36.0
20	35.00094112183307	36.0	36.0	36.0	36.0	36.0
21	34.99442855874819	36.0	36.0	36.0	36.0	36.0
22	34.97446052915219	36.0	36.0	36.0	36.0	36.0
23	34.927488445005196	36.0	36.0	36.0	32.0	36.0
24	34.886440078871956	36.0	36.0	36.0	32.0	36.0
25	34.85977879079912	36.0	36.0	36.0	32.0	36.0
26	34.802840325505315	36.0	36.0	36.0	32.0	36.0
27	34.794618685171564	36.0	36.0	36.0	32.0	36.0
28	34.768900500240925	36.0	36.0	36.0	32.0	36.0
29	34.73764733014633	36.0	36.0	36.0	32.0	36.0
30	34.71215541071746	36.0	36.0	36.0	32.0	36.0
31	34.71466335180442	36.0	36.0	36.0	32.0	36.0
32	34.67391297456316	36.0	36.0	36.0	32.0	36.0
33	34.64973941822424	36.0	36.0	36.0	32.0	36.0
34	34.646698901245216	36.0	36.0	36.0	32.0	36.0
35	34.605679462225154	36.0	36.0	36.0	32.0	36.0
36	34.5798118865867	36.0	36.0	36.0	32.0	36.0
37	34.57186445943547	36.0	36.0	36.0	32.0	36.0
38	34.513367496386095	36.0	36.0	36.0	32.0	36.0
39	34.5100083849002	36.0	36.0	36.0	32.0	36.0
40	34.49425103345084	36.0	36.0	36.0	32.0	36.0
41	34.480888292206636	36.0	36.0	36.0	32.0	36.0
42	34.41346592782329	36.0	36.0	36.0	32.0	36.0
43	34.41415502713601	36.0	36.0	36.0	32.0	36.0
44	34.33077202898735	36.0	36.0	36.0	32.0	36.0
45	34.308228297532395	36.0	36.0	36.0	32.0	36.0
46	34.267834159671324	36.0	36.0	36.0	32.0	36.0
47	34.25111745834496	36.0	36.0	36.0	32.0	36.0
48	34.193661078846596	36.0	36.0	36.0	32.0	36.0
49	34.17267663767087	36.0	36.0	36.0	32.0	36.0
50	33.57232451941366	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	7.0
18	1.0
19	6.0
20	18.0
21	81.0
22	246.0
23	778.0
24	1909.0
25	4524.0
26	9273.0
27	17091.0
28	28645.0
29	43969.0
30	64017.0
31	91529.0
32	131858.0
33	214810.0
34	444852.0
35	1469969.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.32547636767714	17.483551732091183	11.548225663657194	26.64274623657448
2	15.97682493162475	19.91841729230052	37.41740880196103	26.687348974113696
3	18.56303062124169	23.706606698830033	28.692430381502433	29.037932298425844
4	12.04112880728361	15.695653483299942	35.80514855063275	36.458069158783694
5	14.14619049732444	36.980936636149224	33.387634412010854	15.485238454515482
6	34.982459435587316	35.71911162678085	16.26218160794649	13.036247329685342
7	30.472110487350722	30.248499851203626	20.26444147071842	19.01494819072723
8	28.19146427916181	33.7431738920416	18.68842831799073	19.376933510805866
9	27.49119201002076	13.401657419812718	17.871333405453644	41.23581716471288
10	15.698643425443876	26.967278790851978	31.457677679407126	25.87640010429702
11	38.32256029519921	20.243035302173418	22.297216973954505	19.13718742867287
12	25.025757059415593	23.077611963955967	29.078769050317405	22.817861926311032
13	30.313396793595114	19.108930429620845	25.005844885071728	25.571827891712314
14	23.53042806336249	19.597119166262146	24.73665335882627	32.135799411549094
15	25.272409028243676	27.864004916974537	21.722383527976785	25.141202526805
16	25.786028692662622	25.391587796979838	24.183340122651543	24.639043387705996
17	24.094977619132155	25.82137150972585	25.126803783824908	24.956847087317087
18	24.92930666641306	24.698166411473245	26.518281993488607	23.854244928625093
19	26.16063199055945	24.163351933878115	24.83743345768039	24.838582617882043
20	26.181182863021146	24.216982911523825	24.6231532129854	24.978681012469625
21	27.239156691435674	23.86082650706297	23.90663437396972	24.99338242753164
22	26.320069108171722	24.301428922403886	24.72776769509982	24.65073427432457
23	24.76233989892065	24.087856767537314	25.299081219324115	25.850722114217916
24	25.339984759679645	24.593224912811973	25.049879753634634	25.016910573873748
25	25.360257384161873	24.197210033670917	25.263092406192406	25.17944017597481
26	24.501642401500494	25.377312469213642	25.718117220190383	24.402927909095485
27	25.34443984315093	24.526748155794955	25.08348705836664	25.045324942687476
28	24.57936114489378	24.851009497587263	25.327137135459203	25.24249222205975
29	24.599339891096694	24.358842776546915	25.195728352782993	25.846088979573402
30	24.33191571652525	24.931761009778544	25.641573316890458	25.09474995680575
31	25.5369739240707	24.45430768011413	24.522945232622654	25.485773163192516
32	25.154616787735435	24.820358415326034	24.41148136890989	25.613543428028645
33	24.586195295134917	24.421065729651158	25.367578729790253	25.625160245423668
34	25.33221689540963	24.661363454437524	25.41487979799501	24.591539852157833
35	25.653626175401694	24.47446977391313	25.56684191275685	24.305062137928328
36	24.309302716343716	25.201910761387847	25.106919574921378	25.381866947347064
37	25.450260612019182	24.866702093891657	24.73969509602403	24.943342198065135
38	25.006419940175668	24.85337173672856	24.889236217339544	25.25097210575623
39	25.759101430288446	24.098733612392742	24.658358288979848	25.483806668338964
40	26.308101808463803	24.588165037528885	24.909699654015437	24.19403349999188
41	24.80156481449926	24.744857725043133	25.89508831802255	24.558489142435054
42	26.076717229496655	25.328676887546674	25.014909687909025	23.579696195047646
43	24.959164539365702	24.370032280025455	25.48865840156415	25.18214477904469
44	24.40348984150521	25.11510556742815	24.902349980364985	25.579054610701657
45	24.584077655745006	25.282380204378914	25.048305005492267	25.085237134383814
46	24.64995851095953	24.562780011396608	24.975728713189866	25.811532764453997
47	25.295112752904963	24.521077048115593	25.267809696508102	24.91600050247134
48	25.708761686548282	25.0752024651271	24.200393022289095	25.015642826035528
49	25.03673370402541	25.20455165067872	24.637180373185902	25.12153427210996
50	25.027738635481043	25.618452691360922	24.25743672817247	25.096371944985567
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	26.0
1	28.0
2	30.0
3	133.5
4	237.0
5	266.0
6	295.0
7	291.5
8	288.0
9	368.0
10	448.0
11	710.5
12	973.0
13	1967.0
14	2961.0
15	3966.0
16	4971.0
17	5524.0
18	6077.0
19	6057.0
20	6037.0
21	6788.5
22	7540.0
23	8621.0
24	9702.0
25	11915.5
26	14129.0
27	18721.0
28	23313.0
29	27744.5
30	32176.0
31	36800.5
32	41425.0
33	49323.5
34	57222.0
35	67822.5
36	78423.0
37	88721.0
38	99019.0
39	102972.5
40	106926.0
41	114164.0
42	121402.0
43	124235.5
44	127069.0
45	140412.0
46	153755.0
47	165101.5
48	176448.0
49	184428.5
50	192409.0
51	187397.0
52	182385.0
53	180179.0
54	177973.0
55	181041.0
56	184109.0
57	180106.5
58	176104.0
59	161672.0
60	147240.0
61	130617.5
62	113995.0
63	99231.5
64	84468.0
65	72054.0
66	59640.0
67	51825.0
68	44010.0
69	39880.0
70	35750.0
71	27331.5
72	18913.0
73	16442.0
74	13971.0
75	10723.0
76	7475.0
77	6526.5
78	5578.0
79	4635.5
80	3693.0
81	2983.5
82	2274.0
83	1825.0
84	1376.0
85	996.5
86	617.0
87	446.5
88	276.0
89	213.0
90	150.0
91	122.0
92	94.0
93	81.0
94	68.0
95	65.0
96	62.0
97	48.5
98	35.0
99	31.0
100	27.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008638507773071949
2	3.962618244528417E-4
3	3.5663564200755747E-4
4	0.0
5	0.0
6	1.9813091222642084E-4
7	3.962618244528417E-5
8	3.962618244528417E-5
9	9.114021962415359E-4
10	8.717760137962516E-4
11	0.0
12	1.5850472978113668E-4
13	3.962618244528417E-4
14	3.5663564200755747E-4
15	0.0032097207780680177
16	4.7551418934341E-4
17	0.0
18	3.1700945956227337E-4
19	7.925236489056834E-5
20	4.7551418934341E-4
21	0.0
22	1.5850472978113668E-4
23	8.717760137962516E-4
24	5.943927366792625E-4
25	0.0016246734802566507
26	0.005745796454566204
27	0.005508039359894499
28	0.0040022444269737015
29	0.0024171971291623343
30	0.003962618244528417
31	0.007291217569932287
32	0.0034474778727397225
33	0.004398506251426543
34	0.0032493469605133015
35	0.003368225507849154
36	0.00760822702949456
37	0.0040022444269737015
38	0.007766731759275697
39	0.004240001521645406
40	0.0024171971291623343
41	0.003566356420075575
42	0.0022586923993811975
43	0.001664299662701935
44	0.001228411655803809
45	0.0014265425680302299
46	0.0010302807435773884
47	0.0022586923993811975
48	0.0024964494940529023
49	6.340189191245467E-4
50	0.0011095331084679566
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2523584.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.473416739878346
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.29783859281517	31.96682910243787
2	15.523377799532309	14.118020557774166
3	5.981838664064932	8.160439273252255
4	2.7941224238559594	5.0823317360896425
5	1.558296934090582	3.543054294418789
6	0.947192857884491	2.58432573357707
7	0.5974461053007196	1.9017541008169807
8	0.4178599679116506	1.5201216367806942
9	0.3075295444271413	1.2585977220204296
>10	1.3863523031197966	10.984358124460709
>50	0.0901485074371663	2.855213579011769
>100	0.07927056309327478	7.51156092848819
>500	0.01239591005554952	3.9512676201570405
>1k	0.006329826411343895	4.562125590714331
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	2901	0.11495555527376937	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	2800	0.11095331084679566	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	2740	0.10857573990007861	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	2688	0.10651517841292384	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	2674	0.10596041185868986	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	2652	0.10508863584489361	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.5850472978113666E-4
2	0.0	0.0	0.0	0.0	1.5850472978113666E-4
3	0.0	0.0	0.0	0.0	1.5850472978113666E-4
4	0.0	0.0	0.0	0.0	1.5850472978113666E-4
5	0.0	0.0	0.0	0.0	1.5850472978113666E-4
6	0.0	0.0	0.0	0.0	1.9813091222642084E-4
7	0.0	0.0	0.0	0.0	1.9813091222642084E-4
8	0.0	0.0	0.0	0.0	1.9813091222642084E-4
9	0.0	0.0	0.0	0.0	1.9813091222642084E-4
10	0.0	0.0	0.0	0.0	1.9813091222642084E-4
11	0.0	0.0	0.0	3.9626182445284164E-5	2.37757094671705E-4
12	0.0	0.0	0.0	3.9626182445284164E-5	7.13271284015115E-4
13	0.0	0.0	0.0	3.9626182445284164E-5	8.717760137962517E-4
14	0.0	0.0	0.0	7.925236489056833E-5	8.717760137962517E-4
15	0.0	0.0	0.0	7.925236489056833E-5	0.0011491592909132408
16	0.0	0.0	0.0	7.925236489056833E-5	0.001188785473358525
17	0.0	0.0	0.0	7.925236489056833E-5	0.001188785473358525
18	0.0	0.0	0.0	7.925236489056833E-5	0.0012680378382490933
19	0.0	0.0	0.0	7.925236489056833E-5	0.0012680378382490933
20	0.0	0.0	0.0	1.9813091222642084E-4	0.0015057949329207983
21	0.0	0.0	0.0	3.566356420075575E-4	0.001664299662701935
22	0.0	0.0	0.0	4.7551418934341E-4	0.0017435520275925034
23	0.0	0.0	0.0	9.906545611321042E-4	0.0017435520275925034
24	0.0	0.0	0.0	0.001664299662701935	0.0017831782100377876
25	3.9626182445284164E-5	0.0	0.0	0.0022190662169359133	0.0017831782100377876
26	3.9626182445284164E-5	0.0	0.0	0.0024964494940529027	0.0018624305749283558
27	3.9626182445284164E-5	0.0	0.0	0.0028927113185057444	0.0020209353047094926
28	3.9626182445284164E-5	0.0	0.0	0.004913646623215237	0.0020209353047094926
29	3.9626182445284164E-5	0.0	0.0	0.009193274327305927	0.002060561487154777
30	3.9626182445284164E-5	0.0	0.0	0.016920379904136338	0.002100187669600061
31	3.9626182445284164E-5	0.0	0.0	0.034197395450280235	0.002100187669600061
32	3.9626182445284164E-5	0.0	0.0	0.055754038700514826	0.002139813852045345
33	3.9626182445284164E-5	0.0	0.0	0.07592376556516446	0.002179440034490629
34	3.9626182445284164E-5	0.0	0.0	0.09882769901853872	0.0024964494940529027
35	3.9626182445284164E-5	0.0	0.0	0.12418845578352058	0.0024964494940529027
36	3.9626182445284164E-5	0.0	0.0	0.164409030965484	0.0024964494940529027
37	3.9626182445284164E-5	0.0	0.0	0.22440307118764424	0.0024964494940529027
38	3.9626182445284164E-5	0.0	0.0	0.3034176789835409	0.002654954223834039
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	4090	0.0	23.774687	1
TCTATAC	210	0.0	20.95039	3
TACGATA	55	0.004487407	19.9981	2
TAGGACG	1290	0.0	19.781067	4
GATTTCG	605	0.0	19.272806	41
GATATAC	1145	0.0	19.21367	1
GCGAGAA	960	0.0	18.562944	20
AGGACGT	1415	0.0	18.500008	5
GTAGGAC	1645	0.0	18.454172	3
ATTTCGT	610	0.0	18.393154	42
TTAGGAC	945	0.0	18.389788	3
TGGCGAG	1690	0.0	18.35365	18
GTCCTAC	1635	0.0	18.299404	1
TATGGCG	1615	0.0	18.115927	16
GACGTGA	790	0.0	18.099546	7
TGTAGGA	1710	0.0	18.009985	2
TCCTACA	1780	0.0	17.919645	2
GTCCTAA	880	0.0	17.749722	1
ATACGAA	860	0.0	17.650885	40
GGTATCA	2160	0.0	17.620092	1
>>END_MODULE