FastQCFastQC Report
Thu 2 Feb 2017
SRR4064107_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064107_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences785429
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT31770.4044923220303809No Hit
TATCAACGCAGAGTACTTTTTTTTT22500.2864676501631593No Hit
GTACTTTTTTTTTTTTTTTTTTTTT19130.2435611621164994No Hit
GTCCTAAAGTGTGTATTTCTCATTT14630.18626763208386754No Hit
GTACATGGGGTGGTATCAACGCAAA13390.17048008158598676No Hit
GAGTACTTTTTTTTTTTTTTTTTTT12200.15532912586624634No Hit
CTGTAGGACGTGGAATATGGCAAGA11920.15176419510866038No Hit
GTCCTACAGTGGACATTTCTAAATT11540.14692607479479367No Hit
ACGCAGAGTACTTTTTTTTTTTTTT11500.14641679897228138No Hit
CTTTAGGACGTGAAATATGGCGAGG11400.14514360941600069No Hit
GGTATCAACGCAGAGTACTTTTTTT11230.14297918717032346No Hit
GTATCAACGCAGAGTACATGGGGTG10010.12744627458369884No Hit
GTCCTACAGTGTGCATTTCTCATTT9670.12311743009234444No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG8190.10427422465938997No Hit
TTGTAGAACAGTGTATATCAATGAG8170.10401958674813382No Hit
GATATACACTGTTCTACAAATCCCG8150.10376494883687767No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGTTT907.2759576E-1216.89047412
GTATAGA350.002176797316.276871
CGTCGTA1055.456968E-1215.38043710
CGGTTTC1004.0017767E-1115.20142813
CCCTTAT456.7849434E-414.7697521
CCGTCGT1101.2732926E-1114.6822619
TCCAACG1750.014.66038818
TCGCAGT400.005266046614.25406516
CGAATCT400.005266046614.25406516
CCAACGT759.631058E-713.93641919
GCCGGTT1101.8553692E-1013.81859911
ACCGTCG1257.2759576E-1213.6804138
TAGAAAT1850.013.3500534
CTTAGAC500.001501654413.29616453
TACCGTC1301.4551915E-1113.15424257
GTATTAG1301.4551915E-1113.1467021
GTCGTAG1251.0186341E-1012.9203911
CGCCAGT905.379079E-712.66947218
CCAACGA1207.348717E-1012.66947119
ATACCGT1352.7284841E-1112.6670486
Produced by FastQC (version 0.11.5)