##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064107_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	785429
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.172430607986207	32.0	32.0	32.0	32.0	32.0
2	30.7838658873049	32.0	32.0	32.0	32.0	32.0
3	30.80869053727326	32.0	32.0	32.0	32.0	32.0
4	30.830090307335226	32.0	32.0	32.0	32.0	32.0
5	30.7488875506252	32.0	32.0	32.0	32.0	32.0
6	34.39223914574074	36.0	36.0	36.0	32.0	36.0
7	34.311819400607824	36.0	36.0	36.0	32.0	36.0
8	34.26077723129653	36.0	36.0	36.0	32.0	36.0
9	34.38154308027842	36.0	36.0	36.0	32.0	36.0
10	34.089221304535485	36.0	36.0	36.0	32.0	36.0
11	34.39089211119019	36.0	36.0	36.0	32.0	36.0
12	34.19039658581489	36.0	36.0	36.0	32.0	36.0
13	34.277010398139105	36.0	36.0	36.0	32.0	36.0
14	34.1934102254946	36.0	36.0	36.0	32.0	36.0
15	34.13121109610162	36.0	36.0	36.0	32.0	36.0
16	34.125914627547495	36.0	36.0	36.0	32.0	36.0
17	34.05170550107012	36.0	36.0	36.0	32.0	36.0
18	34.04544522802188	36.0	36.0	36.0	32.0	36.0
19	34.037338830117044	36.0	36.0	36.0	32.0	36.0
20	34.013406686027636	36.0	36.0	36.0	32.0	36.0
21	33.98375664764097	36.0	36.0	36.0	32.0	36.0
22	33.963092781142535	36.0	36.0	36.0	32.0	36.0
23	33.93799184904046	36.0	36.0	36.0	32.0	36.0
24	33.89851915322709	36.0	36.0	36.0	32.0	36.0
25	33.53806645794846	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	8.0
4	5.0
5	7.0
6	31.0
7	11.0
8	39.0
9	61.0
10	75.0
11	21.0
12	44.0
13	44.0
14	80.0
15	143.0
16	263.0
17	330.0
18	408.0
19	639.0
20	1031.0
21	1402.0
22	2168.0
23	3386.0
24	4805.0
25	6801.0
26	9674.0
27	12735.0
28	16771.0
29	22145.0
30	28883.0
31	38808.0
32	53832.0
33	74850.0
34	160240.0
35	345689.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.39287488377425	17.42074359007018	11.572391129905364	25.61399039625021
2	15.652248095179889	20.045557796520395	39.76999551807033	24.53219859022939
3	18.89010976418716	25.00588863665011	28.886659341414877	27.217342257747855
4	12.083520076598692	15.789567502597428	36.520998431356574	35.60591398944731
5	13.765749247155746	37.614072616842066	33.83094269470494	14.789235441297247
6	32.80595418486496	36.57978174780029	17.500031833751418	13.114232233583333
7	29.252191010806694	30.71452039722179	21.212117353111225	18.821171238860295
8	27.260256874003353	34.409373200939456	19.434566416857294	18.895803508199897
9	27.91313552575095	13.644545048080413	18.758685874204975	39.68363355196366
10	16.073241668715596	26.754891631834877	31.916918377044524	25.254948322405006
11	36.80310326377742	20.628805829447884	23.886850620397972	18.681240286376724
12	25.516350460931648	23.295648540255716	29.950747325883004	21.23725367292963
13	30.159213474986657	19.917545842193036	25.561171124227787	24.36206955859252
14	23.194362514954346	20.594122226200138	25.575208723781323	30.636306535064193
15	24.26342160680912	29.076612815897125	22.632610864172996	24.027354713120758
16	24.22528643666797	25.854404939209047	25.869948261122712	24.05036036300027
17	22.686748185197736	26.385785301694153	26.48846909421684	24.43899741889127
18	23.4366299834231	25.045774419075794	28.76391867252274	22.75367692497837
19	25.607482827644468	24.27140922255194	25.865498186247592	24.255609763556
20	25.825543821736556	24.817027175796618	25.72411877433691	23.633310228129904
21	26.563156969321682	23.917696794563064	24.669683788322413	24.84946244779284
22	25.569397353026996	25.444400697738722	25.282070688730297	23.70413126050399
23	23.759306712811057	25.088846216087756	26.47521671595842	24.67663035514276
24	24.865860790832027	25.092027588526783	26.45769279286052	23.584418827780674
25	24.430193622042026	25.076322455366018	26.57958176823457	23.913902154357388
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	107.0
1	107.0
2	316.5
3	526.0
4	526.0
5	526.0
6	1460.0
7	2394.0
8	2394.0
9	2394.0
10	2245.0
11	2096.0
12	2096.0
13	2096.0
14	1974.0
15	1852.0
16	1852.0
17	1852.0
18	3430.5
19	5009.0
20	5009.0
21	5009.0
22	8959.0
23	12909.0
24	12909.0
25	12909.0
26	20666.0
27	28423.0
28	28423.0
29	28423.0
30	37367.0
31	46311.0
32	46311.0
33	46311.0
34	52154.0
35	57997.0
36	57997.0
37	57997.0
38	62237.5
39	66478.0
40	66478.0
41	66478.0
42	74420.0
43	82362.0
44	82362.0
45	82362.0
46	91810.5
47	101259.0
48	101259.0
49	101259.0
50	101989.5
51	102720.0
52	102720.0
53	102720.0
54	92910.5
55	83101.0
56	83101.0
57	83101.0
58	76205.5
59	69310.0
60	69310.0
61	69310.0
62	60889.0
63	52468.0
64	52468.0
65	52468.0
66	43300.5
67	34133.0
68	34133.0
69	34133.0
70	25552.5
71	16972.0
72	16972.0
73	16972.0
74	13059.5
75	9147.0
76	9147.0
77	9147.0
78	7293.5
79	5440.0
80	5440.0
81	5440.0
82	3732.5
83	2025.0
84	2025.0
85	2025.0
86	1610.0
87	1195.0
88	1195.0
89	1195.0
90	856.5
91	518.0
92	518.0
93	518.0
94	372.0
95	226.0
96	226.0
97	226.0
98	338.5
99	451.0
100	451.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04061474684535457
2	0.006747904648287751
3	0.002291741201305274
4	0.004710801358238619
5	0.009421602716477238
6	0.012604576607179008
7	0.022280817234912386
8	0.03781372982153702
9	0.049654392694947605
10	0.05933063332268098
11	0.05716621107700378
12	0.06327752094715118
13	0.06467802945905995
14	0.06875223603915823
15	0.06136773661273011
16	0.06696977066036522
17	0.06302288303589504
18	0.07664601128809861
19	0.0753728217318179
20	0.07804651980000739
21	0.07320839948614069
22	0.07740992502186703
23	0.08224804533573372
24	0.07766456293312317
25	0.07651869233247054
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	785429.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.822590275862886
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.78941540638085	35.28800979674796
2	14.630957148676579	13.993805381297342
3	5.002051818209765	7.176332239226416
4	2.20983583349972	4.227202945695082
5	1.1404806352514232	2.7270369068592317
6	0.7285320837323034	2.0904174805890343
7	0.47355068141175455	1.5852494148407015
8	0.32106508736713923	1.2283331300034255
9	0.2281013944265482	0.9817559574312426
>10	1.2223315816013511	10.889091018399625
>50	0.12670569284611913	4.240167145739248
>100	0.11547827159952861	10.731872331345075
>500	0.008286635230369165	2.5241011921276453
>1k	0.003207729766594516	2.316625059698009
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3177	0.4044923220303809	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2250	0.2864676501631593	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1913	0.2435611621164994	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	1463	0.18626763208386754	No Hit
GTACATGGGGTGGTATCAACGCAAA	1339	0.17048008158598676	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1220	0.15532912586624634	No Hit
CTGTAGGACGTGGAATATGGCAAGA	1192	0.15176419510866038	No Hit
GTCCTACAGTGGACATTTCTAAATT	1154	0.14692607479479367	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1150	0.14641679897228138	No Hit
CTTTAGGACGTGAAATATGGCGAGG	1140	0.14514360941600069	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1123	0.14297918717032346	No Hit
GTATCAACGCAGAGTACATGGGGTG	1001	0.12744627458369884	No Hit
GTCCTACAGTGTGCATTTCTCATTT	967	0.12311743009234444	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	819	0.10427422465938997	No Hit
TTGTAGAACAGTGTATATCAATGAG	817	0.10401958674813382	No Hit
GATATACACTGTTCTACAAATCCCG	815	0.10376494883687767	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	1.2731895562807078E-4
13	0.0	0.0	0.0	0.0	1.2731895562807078E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGGTTT	90	7.2759576E-12	16.890474	12
GTATAGA	35	0.0021767973	16.27687	1
CGTCGTA	105	5.456968E-12	15.380437	10
CGGTTTC	100	4.0017767E-11	15.201428	13
CCCTTAT	45	6.7849434E-4	14.769752	1
CCGTCGT	110	1.2732926E-11	14.682261	9
TCCAACG	175	0.0	14.660388	18
TCGCAGT	40	0.0052660466	14.254065	16
CGAATCT	40	0.0052660466	14.254065	16
CCAACGT	75	9.631058E-7	13.936419	19
GCCGGTT	110	1.8553692E-10	13.818599	11
ACCGTCG	125	7.2759576E-12	13.680413	8
TAGAAAT	185	0.0	13.350053	4
CTTAGAC	50	0.0015016544	13.2961645	3
TACCGTC	130	1.4551915E-11	13.1542425	7
GTATTAG	130	1.4551915E-11	13.146702	1
GTCGTAG	125	1.0186341E-10	12.92039	11
CGCCAGT	90	5.379079E-7	12.669472	18
CCAACGA	120	7.348717E-10	12.669471	19
ATACCGT	135	2.7284841E-11	12.667048	6
>>END_MODULE