FastQCFastQC Report
Thu 2 Feb 2017
SRR4064107_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064107_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences785429
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8980.11433242215400756No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA8240.10491081943753032No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA8150.10376494883687767No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACGAG353.2165716E-431.42902635
CGTAAAT701.1319116E-625.14161916
TCTAGAT1900.021.9989172
GTATTAC654.927451E-420.3092781
CTACTAG550.004481863720.00028835
CTATACC550.004483515419.9990164
ATACACT4500.019.5545944
TTAGGAC5300.019.5084743
CTAGGAC1705.456968E-1219.4108093
TGTAGGA9300.019.3968962
CACGTAT808.973686E-519.2527328
CTAGATA1950.019.1785453
GATATAC4600.019.131931
CCTAATA708.1225514E-418.8562152
CTTGCAC708.1225514E-418.8562153
TAGGACC7600.018.8148634
ACTGTTC4800.018.7907438
AATCGTA951.5966256E-518.52540413
GTAGGAC9050.018.474233
GTCCTAC7150.018.462981
Produced by FastQC (version 0.11.5)