##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064107_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	785429
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.27044328640781	32.0	32.0	32.0	32.0	32.0
2	31.417720761520137	32.0	32.0	32.0	32.0	32.0
3	31.486087221123743	32.0	32.0	32.0	32.0	32.0
4	31.564319626598966	32.0	32.0	32.0	32.0	32.0
5	31.521753080163833	32.0	32.0	32.0	32.0	32.0
6	35.0906332717534	36.0	36.0	36.0	36.0	36.0
7	35.11881914215034	36.0	36.0	36.0	36.0	36.0
8	35.06706907944576	36.0	36.0	36.0	36.0	36.0
9	35.16947553502608	36.0	36.0	36.0	36.0	36.0
10	35.02940940556053	36.0	36.0	36.0	36.0	36.0
11	35.17414432112896	36.0	36.0	36.0	36.0	36.0
12	35.07856088838074	36.0	36.0	36.0	36.0	36.0
13	35.10901430937742	36.0	36.0	36.0	36.0	36.0
14	35.06090174923513	36.0	36.0	36.0	36.0	36.0
15	35.03647688078744	36.0	36.0	36.0	36.0	36.0
16	35.05111346792645	36.0	36.0	36.0	36.0	36.0
17	35.01263640634608	36.0	36.0	36.0	36.0	36.0
18	35.0086666013096	36.0	36.0	36.0	36.0	36.0
19	35.00369606928188	36.0	36.0	36.0	36.0	36.0
20	34.99515678692791	36.0	36.0	36.0	36.0	36.0
21	34.97963915261596	36.0	36.0	36.0	36.0	36.0
22	34.963134796397895	36.0	36.0	36.0	36.0	36.0
23	34.92567374008345	36.0	36.0	36.0	36.0	36.0
24	34.89208445320965	36.0	36.0	36.0	32.0	36.0
25	34.864645945082245	36.0	36.0	36.0	32.0	36.0
26	34.80632495107769	36.0	36.0	36.0	32.0	36.0
27	34.7964640979643	36.0	36.0	36.0	32.0	36.0
28	34.75754142003924	36.0	36.0	36.0	32.0	36.0
29	34.733147108140905	36.0	36.0	36.0	32.0	36.0
30	34.703382482694174	36.0	36.0	36.0	32.0	36.0
31	34.690451969560584	36.0	36.0	36.0	32.0	36.0
32	34.653547297082234	36.0	36.0	36.0	32.0	36.0
33	34.629001475626694	36.0	36.0	36.0	32.0	36.0
34	34.5925233216497	36.0	36.0	36.0	32.0	36.0
35	34.55449951555137	36.0	36.0	36.0	32.0	36.0
36	34.51368105837702	36.0	36.0	36.0	32.0	36.0
37	34.48831784922635	36.0	36.0	36.0	32.0	36.0
38	34.43032024536909	36.0	36.0	36.0	32.0	36.0
39	34.39314820308392	36.0	36.0	36.0	32.0	36.0
40	34.39887882927674	36.0	36.0	36.0	32.0	36.0
41	34.36168259639	36.0	36.0	36.0	32.0	36.0
42	34.28888161756187	36.0	36.0	36.0	32.0	36.0
43	34.286979472364784	36.0	36.0	36.0	32.0	36.0
44	34.18762357896131	36.0	36.0	36.0	32.0	36.0
45	34.13974655888693	36.0	36.0	36.0	32.0	36.0
46	34.06364292634981	36.0	36.0	36.0	32.0	36.0
47	34.02631937450744	36.0	36.0	36.0	32.0	36.0
48	33.963478557578085	36.0	36.0	36.0	32.0	36.0
49	33.91520939512037	36.0	36.0	36.0	32.0	36.0
50	33.33691905952034	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	5.0
20	6.0
21	28.0
22	99.0
23	267.0
24	658.0
25	1706.0
26	3254.0
27	6043.0
28	10008.0
29	15038.0
30	21229.0
31	29737.0
32	42102.0
33	66834.0
34	135229.0
35	453186.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.66468160745231	18.21046720832426	12.055637682764873	26.069213501458556
2	14.965999386321071	20.756989510164534	39.09032920946186	25.186681894052537
3	18.343930554883592	25.455739942400683	29.130433675483115	27.06989582723261
4	11.649811758924104	16.319107137627974	36.736866094834795	35.29421500861313
5	13.303557673577115	38.0844099212023	33.61144037207692	15.00059203314367
6	33.01367665445249	36.680221943251176	17.123828342835605	13.182273059460725
7	29.011533824190344	31.046345373038175	21.215412214216688	18.72670858855479
8	27.245492590673376	34.520879672128224	19.495842399503964	18.737785337694433
9	27.84281021619006	13.493417534567492	18.688981691324386	39.974790557918055
10	15.885305261871293	27.14164578622502	31.932561266271463	25.04048768563222
11	36.87144223093367	20.61561261425285	23.885927308515473	18.62701784629801
12	25.375049813541388	23.428724938855073	29.91167884048081	21.284546407122733
13	30.164000799569152	19.90201458322458	25.652801102081295	24.281183515124972
14	23.162275854693238	20.5592364927148	25.692743319565892	30.58574433302607
15	24.396607044635036	29.13258602073869	22.595086275388596	23.875720659237682
16	24.36654991399517	25.792154780684502	25.91209045849296	23.92920484682737
17	22.653734455947006	26.447330057841	26.49609321784655	24.40284226836544
18	23.489710718600918	24.86882964596418	28.879631386159666	22.761828249275236
19	25.66311063918098	24.12547569665927	26.127189414165798	24.08422424999395
20	25.769680796207904	24.676893852102623	26.05093051769556	23.50249483399391
21	26.594256132635795	23.77261343800649	25.04058291710645	24.59254751225127
22	25.65633005189527	25.14679894274205	25.68956034162266	23.507310663740025
23	23.738090721234705	24.873538515209116	26.694354718576246	24.69401604497993
24	24.971512311137765	25.00792562516949	26.545560567691208	23.47500149600154
25	24.415537125265143	25.004010582845908	26.758354107728078	23.822098184160872
26	23.208351477093025	26.211302862003024	27.454373219046747	23.125972441857208
27	23.952736255061243	25.657388779953656	26.472535587868297	23.917339377116807
28	23.146765266483065	25.69566728673524	26.636974676501186	24.52059277028051
29	23.745335232980523	24.686882885222857	26.243396752520027	25.324385129276592
30	23.289042128422423	25.47312988917862	26.74877261245111	24.48905536994785
31	24.77937291102976	24.750469520929492	25.79990450421773	24.670253063823015
32	23.82919829590452	25.928887372326525	25.71880387368507	24.523110458083885
33	23.504546108528555	25.36411521816355	26.875834768913048	24.25550390439485
34	24.38465103487614	25.461925521512384	26.77436440677966	23.379059036831812
35	24.456871185875276	25.645560304709814	26.594881863196346	23.302686646218564
36	23.408232308634542	26.040243503372302	26.339211249743748	24.212312938249408
37	24.346602664901102	25.880735171474228	25.4693498176077	24.303312346016973
38	24.030956425189913	26.1641128549637	25.77983146625378	24.02509925359261
39	25.002578307331685	24.965781723684394	25.49468486718216	24.53695510180176
40	25.659784468709258	25.51119927477184	25.878269638200784	22.95074661831812
41	24.321306432596646	25.349564429097814	27.000684996040263	23.328444142265276
42	25.137507448320118	26.723554007323546	25.690847606097183	22.448090938259156
43	23.5572950419968	26.122683873662016	26.419341572693234	23.90067951164795
44	23.339996435028393	26.430062896284788	25.456952967838863	24.772987700847953
45	23.595146545797153	26.707112118357056	25.575615594204375	24.122125741641415
46	23.96829741859503	25.55660947894452	25.246586243116788	25.228506859343668
47	24.97373993045697	25.681138458365215	25.568840851883024	23.776280759294792
48	24.684911798221325	26.18666564809687	24.572614477696504	24.555808075985308
49	24.473280809953298	26.087050627173973	25.09587129565027	24.34379726722246
50	24.13708369301442	27.080993814758507	24.450547351855956	24.331375140371115
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	22.0
1	19.0
2	16.0
3	59.0
4	102.0
5	123.5
6	145.0
7	150.0
8	155.0
9	228.0
10	301.0
11	486.0
12	671.0
13	1250.0
14	1829.0
15	2561.5
16	3294.0
17	3678.0
18	4062.0
19	4026.5
20	3991.0
21	4115.5
22	4240.0
23	4575.5
24	4911.0
25	5235.5
26	5560.0
27	7562.5
28	9565.0
29	11405.0
30	13245.0
31	15349.5
32	17454.0
33	21344.0
34	25234.0
35	29879.5
36	34525.0
37	37210.0
38	39895.0
39	39105.0
40	38315.0
41	38926.5
42	39538.0
43	38773.5
44	38009.0
45	41558.0
46	45107.0
47	47848.0
48	50589.0
49	52802.0
50	55015.0
51	53493.5
52	51972.0
53	51414.0
54	50856.0
55	51430.5
56	52005.0
57	50599.5
58	49194.0
59	45080.5
60	40967.0
61	36108.5
62	31250.0
63	27184.0
64	23118.0
65	19678.5
66	16239.0
67	13652.5
68	11066.0
69	9922.0
70	8778.0
71	6885.0
72	4992.0
73	4227.5
74	3463.0
75	2735.5
76	2008.0
77	1732.5
78	1457.0
79	1198.5
80	940.0
81	758.5
82	577.0
83	469.0
84	361.0
85	278.5
86	196.0
87	142.5
88	89.0
89	66.5
90	44.0
91	37.0
92	30.0
93	23.5
94	17.0
95	13.5
96	10.0
97	7.5
98	5.0
99	5.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007893775248940388
2	2.5463791125614156E-4
3	3.8195686688421234E-4
4	0.0
5	0.0
6	3.8195686688421234E-4
7	0.0
8	0.0
9	0.001145870600652637
10	6.365947781403539E-4
11	0.0
12	0.0
13	7.639137337684247E-4
14	1.2731895562807078E-4
15	0.004710801358238619
16	0.0010185516450245662
17	0.0
18	0.0
19	2.5463791125614156E-4
20	7.639137337684247E-4
21	0.0
22	1.2731895562807078E-4
23	0.0015278274675368493
24	2.5463791125614156E-4
25	8.912326893964955E-4
26	0.004456163446982477
27	0.006238628825775468
28	0.004074206580098265
29	0.0025463791125614156
30	0.002673698068189486
31	0.006875223603915822
32	0.003437611801957911
33	0.004074206580098265
34	0.002673698068189486
35	0.00331029284632984
36	0.007639137337684246
37	0.004328844491354407
38	0.008530370027080741
39	0.003819568668842123
40	0.002673698068189486
41	0.0029283359794456277
42	0.0021644222456772035
43	0.0015278274675368493
44	0.001145870600652637
45	0.001145870600652637
46	5.092758225122831E-4
47	0.0020371032900491325
48	0.001782465378792991
49	1.2731895562807078E-4
50	0.0014005085119087786
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	785429.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.07669991510141
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.16489056063514	39.895043404382704
2	14.224343964978543	15.099624722366997
3	4.694554482975087	7.475143785838881
4	2.055002969553974	4.3629110375863425
5	1.102920489978938	2.926968993841436
6	0.649901804654898	2.0696785835970526
7	0.39808072242625014	1.4790167732343402
8	0.2730953381997297	1.1595999451072148
9	0.21419819176444221	1.0232039831974782
>10	1.0293250669769	9.929477537481846
>50	0.09889385323844943	3.7258575789296646
>100	0.088762493892501	8.828417080210333
>500	0.006030060725147226	2.0250565742257467
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	898	0.11433242215400756	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	824	0.10491081943753032	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	815	0.10376494883687767	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	1.2731895562807078E-4	0.0
10	0.0	0.0	0.0	1.2731895562807078E-4	0.0
11	0.0	0.0	0.0	2.5463791125614156E-4	0.0
12	0.0	0.0	0.0	2.5463791125614156E-4	0.0
13	0.0	0.0	0.0	2.5463791125614156E-4	0.0
14	0.0	0.0	0.0	2.5463791125614156E-4	0.0
15	0.0	0.0	0.0	2.5463791125614156E-4	1.2731895562807078E-4
16	0.0	0.0	0.0	3.8195686688421234E-4	1.2731895562807078E-4
17	0.0	0.0	0.0	3.8195686688421234E-4	1.2731895562807078E-4
18	0.0	0.0	0.0	3.8195686688421234E-4	1.2731895562807078E-4
19	0.0	0.0	0.0	3.8195686688421234E-4	1.2731895562807078E-4
20	0.0	0.0	0.0	5.092758225122831E-4	2.5463791125614156E-4
21	0.0	0.0	0.0	5.092758225122831E-4	5.092758225122831E-4
22	0.0	0.0	0.0	0.0010185516450245662	5.092758225122831E-4
23	0.0	0.0	0.0	0.0014005085119087786	5.092758225122831E-4
24	0.0	0.0	0.0	0.002164422245677203	5.092758225122831E-4
25	0.0	0.0	0.0	0.0025463791125614156	5.092758225122831E-4
26	0.0	0.0	0.0	0.002673698068189486	6.365947781403539E-4
27	0.0	0.0	0.0	0.0030556549350736987	6.365947781403539E-4
28	0.0	0.0	0.0	0.004201525535726336	7.639137337684247E-4
29	0.0	0.0	0.0	0.0064932667370316095	7.639137337684247E-4
30	0.0	0.0	0.0	0.011840662873410582	7.639137337684247E-4
31	0.0	0.0	0.0	0.023554006791193093	7.639137337684247E-4
32	0.0	0.0	0.0	0.03845032459967737	7.639137337684247E-4
33	0.0	0.0	0.0	0.049781711650575676	7.639137337684247E-4
34	0.0	0.0	0.0	0.06264092616901082	0.0012731895562807078
35	0.0	0.0	0.0	0.07791920084437931	0.0012731895562807078
36	0.0	0.0	0.0	0.10287371614748118	0.0012731895562807078
37	0.0	0.0	0.0	0.13712251521143223	0.0012731895562807078
38	0.0	0.0	0.0	0.1885593732851728	0.0012731895562807078
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCACGAG	35	3.2165716E-4	31.429026	35
CGTAAAT	70	1.1319116E-6	25.141619	16
TCTAGAT	190	0.0	21.998917	2
GTATTAC	65	4.927451E-4	20.309278	1
CTACTAG	55	0.0044818637	20.000288	35
CTATACC	55	0.0044835154	19.999016	4
ATACACT	450	0.0	19.554594	4
TTAGGAC	530	0.0	19.508474	3
CTAGGAC	170	5.456968E-12	19.410809	3
TGTAGGA	930	0.0	19.396896	2
CACGTAT	80	8.973686E-5	19.25273	28
CTAGATA	195	0.0	19.178545	3
GATATAC	460	0.0	19.13193	1
CCTAATA	70	8.1225514E-4	18.856215	2
CTTGCAC	70	8.1225514E-4	18.856215	3
TAGGACC	760	0.0	18.814863	4
ACTGTTC	480	0.0	18.790743	8
AATCGTA	95	1.5966256E-5	18.525404	13
GTAGGAC	905	0.0	18.47423	3
GTCCTAC	715	0.0	18.46298	1
>>END_MODULE