FastQCFastQC Report
Thu 2 Feb 2017
SRR4064106_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064106_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1786701
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT41630.2329992539322472No Hit
TATCAACGCAGAGTACTTTTTTTTT28300.15839247865199604No Hit
GTACTTTTTTTTTTTTTTTTTTTTT21320.11932606518941892No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTA602.5659721E-514.251595515
CCGCTCA751.4780242E-512.6673769
TCTTATA1051.999615E-812.6641862
ACGGTAT2200.012.5234299
CGAACGA1551.8189894E-1212.25909416
GTTCGCG550.003066822212.09192516
CGAAATC550.003068108812.09124813
CGTTATT1650.012.088542
TAAGACT1750.011.940854
CGCCAGT2800.011.875998518
GCGTTAT1459.640644E-1111.7934331
TGGCGAG3400.011.7362818
ATCGCCA2800.011.53668416
GTCGGCG1501.7644197E-1011.40031912
GAACCGC1751.8189894E-1211.399686
TTAGGAC2750.011.3977663
TATTAGG1001.9344734E-611.3977662
AGAACCG1850.011.2957155
CGGTTCT3050.011.21342912
ACGAACG1701.0913936E-1111.17772315
Produced by FastQC (version 0.11.5)