##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064106_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1786701
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.23466937109231	32.0	32.0	32.0	32.0	32.0
2	30.78021056684918	32.0	32.0	32.0	32.0	32.0
3	30.802604912629477	32.0	32.0	32.0	32.0	32.0
4	30.83849452146722	32.0	32.0	32.0	32.0	32.0
5	30.72788843796472	32.0	32.0	32.0	32.0	32.0
6	34.39599071137253	36.0	36.0	36.0	32.0	36.0
7	34.32870413124524	36.0	36.0	36.0	32.0	36.0
8	34.283376457504644	36.0	36.0	36.0	32.0	36.0
9	34.44908633285592	36.0	36.0	36.0	32.0	36.0
10	34.08822572999063	36.0	36.0	36.0	32.0	36.0
11	34.432169120630704	36.0	36.0	36.0	32.0	36.0
12	34.21459438372733	36.0	36.0	36.0	32.0	36.0
13	34.3080174019044	36.0	36.0	36.0	32.0	36.0
14	34.19943739887088	36.0	36.0	36.0	32.0	36.0
15	34.12071185945494	36.0	36.0	36.0	32.0	36.0
16	34.12419817305749	36.0	36.0	36.0	32.0	36.0
17	34.04835000372194	36.0	36.0	36.0	32.0	36.0
18	34.03820169127347	36.0	36.0	36.0	32.0	36.0
19	34.06284095660102	36.0	36.0	36.0	32.0	36.0
20	34.042787237484056	36.0	36.0	36.0	32.0	36.0
21	34.02642803692392	36.0	36.0	36.0	32.0	36.0
22	34.00043935722877	36.0	36.0	36.0	32.0	36.0
23	33.94496449042117	36.0	36.0	36.0	32.0	36.0
24	33.93072315961093	36.0	36.0	36.0	32.0	36.0
25	33.52040212660093	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	5.0
4	22.0
5	30.0
6	70.0
7	25.0
8	114.0
9	101.0
10	166.0
11	45.0
12	106.0
13	104.0
14	196.0
15	261.0
16	510.0
17	611.0
18	865.0
19	1187.0
20	1890.0
21	2750.0
22	4363.0
23	6749.0
24	10043.0
25	15146.0
26	21075.0
27	28097.0
28	37771.0
29	50689.0
30	66841.0
31	90377.0
32	126042.0
33	177864.0
34	372677.0
35	769908.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.50672395663611	17.1523324162011	11.328094817363027	26.012848809799767
2	16.472788272255528	19.910657164078604	37.62860314082439	25.987951422841476
3	18.630612195781307	24.241842492193566	28.74620732346878	28.381337988556343
4	12.462932758989332	15.604398925791635	36.28688393377879	35.645784381440244
5	14.462307299191218	36.788145398326236	33.8749284220154	14.874618880467144
6	33.74358991582715	35.75507306978169	17.211240437002466	13.290096577388699
7	29.94127997232226	30.592468228540653	21.18354809147314	18.28270370766395
8	28.06221116365424	32.94612792727623	19.667746519406553	19.323914389662978
9	27.66600927073849	13.950736742608774	18.515998687447993	39.86725529920474
10	15.688915371442075	26.872106815287548	32.154461482632215	25.284516330638162
11	36.90582791388694	20.998387746199107	22.943172393555688	19.15261194635826
12	24.759036212054855	23.594961294579	29.22749333814234	22.418509155223802
13	29.496000362916032	19.679905821049914	25.274357238186607	25.549736577847447
14	23.600772234939093	19.73220451568133	25.40579019382604	31.261233055553532
15	25.09078160667203	27.290093436170622	22.42686508318754	25.192259873969817
16	25.444952869552555	25.67167188471383	24.20488722857287	24.678488017160745
17	23.946846391739875	25.80342551449364	25.44034248424288	24.809385609523606
18	24.601160809407464	24.969949129168892	26.450871727311732	23.978018334111912
19	25.601473311354646	24.804829934030334	25.460829880259844	24.132866874355173
20	25.763084590880908	24.418426841174558	25.05098619997793	24.767502367966607
21	26.591390031848526	24.29276221821673	24.582009112061325	24.53383863787342
22	25.798017139976476	24.509438189660003	24.8392427043074	24.853301966056122
23	24.368877546447745	24.369717773376234	25.536064780375884	25.725339899800137
24	24.768109837520054	25.018203937875843	25.37729761702051	24.836388607583594
25	24.905940652290443	24.59540617338511	25.35493855685486	25.14371461746958
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	292.0
1	292.0
2	509.5
3	727.0
4	727.0
5	727.0
6	1893.0
7	3059.0
8	3059.0
9	3059.0
10	3332.0
11	3605.0
12	3605.0
13	3605.0
14	4295.0
15	4985.0
16	4985.0
17	4985.0
18	8635.5
19	12286.0
20	12286.0
21	12286.0
22	19522.0
23	26758.0
24	26758.0
25	26758.0
26	39489.5
27	52221.0
28	52221.0
29	52221.0
30	65875.0
31	79529.0
32	79529.0
33	79529.0
34	96978.5
35	114428.0
36	114428.0
37	114428.0
38	131929.5
39	149431.0
40	149431.0
41	149431.0
42	169473.5
43	189516.0
44	189516.0
45	189516.0
46	211703.5
47	233891.0
48	233891.0
49	233891.0
50	237250.0
51	240609.0
52	240609.0
53	240609.0
54	224332.0
55	208055.0
56	208055.0
57	208055.0
58	189841.5
59	171628.0
60	171628.0
61	171628.0
62	149717.0
63	127806.0
64	127806.0
65	127806.0
66	105173.0
67	82540.0
68	82540.0
69	82540.0
70	61940.0
71	41340.0
72	41340.0
73	41340.0
74	31564.0
75	21788.0
76	21788.0
77	21788.0
78	17111.0
79	12434.0
80	12434.0
81	12434.0
82	8566.0
83	4698.0
84	4698.0
85	4698.0
86	3592.0
87	2486.0
88	2486.0
89	2486.0
90	1813.5
91	1141.0
92	1141.0
93	1141.0
94	806.0
95	471.0
96	471.0
97	471.0
98	724.0
99	977.0
100	977.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03912238253630573
2	0.005596907372862051
3	0.0019029485067730975
4	0.0046454331194755024
5	0.010522185860980656
6	0.014440021021984092
7	0.023954763555849582
8	0.03665974329224644
9	0.048525186922713986
10	0.05815186760403672
11	0.05608101187607776
12	0.06509203274638566
13	0.06514800182011428
14	0.06906583698111772
15	0.0613980738802967
16	0.06889792975993185
17	0.06486815645147118
18	0.07807685785142562
19	0.07471871342770839
20	0.07914027025226941
21	0.0769574763768532
22	0.07841267229379734
23	0.08221856930734353
24	0.07684553822939597
25	0.07790895063023975
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1786701.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.24547751398691
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.63195010402637	39.5142734849889
2	14.134775031693806	14.769561421672448
3	4.504105970946773	7.059575016771415
4	1.9459633684113822	4.066711416295163
5	1.0018668158782091	2.6171505100487327
6	0.6016480360946111	1.8860033364669238
7	0.4158447796714656	1.5208206359944079
8	0.286024634413441	1.195479488455501
9	0.20662342919342785	0.9715625751409291
>10	1.104646423731249	10.278027924844755
>50	0.08313103335361265	3.0047502520778666
>100	0.07221201485086262	7.914165823676771
>500	0.008190722960063084	2.9121079266937633
>1k	0.0030176347747600833	2.2898101868724314
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4163	0.2329992539322472	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2830	0.15839247865199604	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2132	0.11932606518941892	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1193814745724102E-4
2	0.0	0.0	0.0	0.0	1.1193814745724102E-4
3	0.0	0.0	0.0	0.0	1.1193814745724102E-4
4	0.0	0.0	0.0	0.0	1.1193814745724102E-4
5	0.0	0.0	0.0	0.0	1.1193814745724102E-4
6	0.0	0.0	0.0	0.0	1.1193814745724102E-4
7	0.0	0.0	0.0	0.0	1.1193814745724102E-4
8	0.0	0.0	0.0	0.0	1.1193814745724102E-4
9	0.0	0.0	0.0	0.0	1.1193814745724102E-4
10	0.0	0.0	0.0	0.0	1.1193814745724102E-4
11	0.0	0.0	0.0	0.0	1.1193814745724102E-4
12	0.0	0.0	0.0	0.0	1.6790722118586153E-4
13	0.0	0.0	0.0	0.0	1.6790722118586153E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCTTA	60	2.5659721E-5	14.2515955	15
CCGCTCA	75	1.4780242E-5	12.667376	9
TCTTATA	105	1.999615E-8	12.664186	2
ACGGTAT	220	0.0	12.523429	9
CGAACGA	155	1.8189894E-12	12.259094	16
GTTCGCG	55	0.0030668222	12.091925	16
CGAAATC	55	0.0030681088	12.091248	13
CGTTATT	165	0.0	12.08854	2
TAAGACT	175	0.0	11.94085	4
CGCCAGT	280	0.0	11.8759985	18
GCGTTAT	145	9.640644E-11	11.793433	1
TGGCGAG	340	0.0	11.73628	18
ATCGCCA	280	0.0	11.536684	16
GTCGGCG	150	1.7644197E-10	11.400319	12
GAACCGC	175	1.8189894E-12	11.39968	6
TTAGGAC	275	0.0	11.397766	3
TATTAGG	100	1.9344734E-6	11.397766	2
AGAACCG	185	0.0	11.295715	5
CGGTTCT	305	0.0	11.213429	12
ACGAACG	170	1.0913936E-11	11.177723	15
>>END_MODULE