##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064106_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1786701
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.327558444306014	32.0	32.0	32.0	32.0	32.0
2	31.435316261646463	32.0	32.0	32.0	32.0	32.0
3	31.503782669847947	32.0	32.0	32.0	32.0	32.0
4	31.583460243208012	32.0	32.0	32.0	32.0	32.0
5	31.543694776014565	32.0	32.0	32.0	32.0	32.0
6	35.13352989672027	36.0	36.0	36.0	36.0	36.0
7	35.150617254929614	36.0	36.0	36.0	36.0	36.0
8	35.10071131095802	36.0	36.0	36.0	36.0	36.0
9	35.196268429916366	36.0	36.0	36.0	36.0	36.0
10	35.06223760998623	36.0	36.0	36.0	36.0	36.0
11	35.20354664826404	36.0	36.0	36.0	36.0	36.0
12	35.10659645906058	36.0	36.0	36.0	36.0	36.0
13	35.14703131637582	36.0	36.0	36.0	36.0	36.0
14	35.08961040487468	36.0	36.0	36.0	36.0	36.0
15	35.064126566224566	36.0	36.0	36.0	36.0	36.0
16	35.0797441765578	36.0	36.0	36.0	36.0	36.0
17	35.04300271841791	36.0	36.0	36.0	36.0	36.0
18	35.03358536207233	36.0	36.0	36.0	36.0	36.0
19	35.03996303802371	36.0	36.0	36.0	36.0	36.0
20	35.0280203570715	36.0	36.0	36.0	36.0	36.0
21	35.02708567354023	36.0	36.0	36.0	36.0	36.0
22	35.003983878668	36.0	36.0	36.0	36.0	36.0
23	34.95587566134457	36.0	36.0	36.0	36.0	36.0
24	34.924518987788105	36.0	36.0	36.0	36.0	36.0
25	34.89930212161968	36.0	36.0	36.0	32.0	36.0
26	34.83935084829527	36.0	36.0	36.0	32.0	36.0
27	34.82766898322663	36.0	36.0	36.0	32.0	36.0
28	34.809291537867836	36.0	36.0	36.0	32.0	36.0
29	34.77837981844752	36.0	36.0	36.0	32.0	36.0
30	34.76391629041457	36.0	36.0	36.0	32.0	36.0
31	34.74913261927989	36.0	36.0	36.0	32.0	36.0
32	34.716743876003875	36.0	36.0	36.0	32.0	36.0
33	34.68823043139283	36.0	36.0	36.0	32.0	36.0
34	34.68555287090565	36.0	36.0	36.0	32.0	36.0
35	34.6364086660275	36.0	36.0	36.0	32.0	36.0
36	34.60968791084798	36.0	36.0	36.0	32.0	36.0
37	34.59754206215813	36.0	36.0	36.0	32.0	36.0
38	34.556710943800894	36.0	36.0	36.0	32.0	36.0
39	34.554262856516004	36.0	36.0	36.0	32.0	36.0
40	34.530553237503085	36.0	36.0	36.0	32.0	36.0
41	34.51545837831848	36.0	36.0	36.0	32.0	36.0
42	34.44411627910882	36.0	36.0	36.0	32.0	36.0
43	34.42909417972005	36.0	36.0	36.0	32.0	36.0
44	34.38149975849345	36.0	36.0	36.0	32.0	36.0
45	34.336396520738504	36.0	36.0	36.0	32.0	36.0
46	34.32041455173529	36.0	36.0	36.0	32.0	36.0
47	34.282520130676595	36.0	36.0	36.0	32.0	36.0
48	34.24305241895538	36.0	36.0	36.0	32.0	36.0
49	34.229404920017394	36.0	36.0	36.0	32.0	36.0
50	33.63874593454641	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	1.0
19	3.0
20	20.0
21	61.0
22	193.0
23	582.0
24	1484.0
25	3533.0
26	6884.0
27	12501.0
28	20445.0
29	31091.0
30	44371.0
31	61982.0
32	89387.0
33	141694.0
34	296698.0
35	1075769.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.52324210681865	17.968063443232793	11.921242265784963	26.587452184163595
2	15.756699244526484	20.649031115568757	37.13181999827614	26.462449641628616
3	17.835033024625886	24.71969225895605	29.088759873666326	28.356514842751736
4	12.086241626326958	16.246590783796506	36.376539779179616	35.290627810696925
5	14.031278876543976	37.151151759583726	33.69489354962022	15.122675814252077
6	33.78215084275716	36.03832775039948	16.99098055348003	13.188540853363333
7	29.71323093593269	30.70075037821144	21.305939052968633	18.28007963288724
8	27.994546364298834	33.13503457215489	19.554116028562184	19.316303034984088
9	27.64253943485155	13.778564836140308	18.377203317212192	40.20169241179595
10	15.504812266746665	27.04328241591686	32.237149938097616	25.214755379238856
11	37.091208881620375	20.887658315521175	22.92621988793872	19.094912914919732
12	24.676512384010962	23.642090805446244	29.221374165430213	22.46002264511258
13	29.641074319986704	19.55736100158225	25.266120144871003	25.535444533560046
14	23.515080349427098	19.588782870728988	25.514860950044106	31.381275829799808
15	25.089399905408772	27.453844797622345	22.342112406843032	25.11464289012585
16	25.534970590819768	25.55075399328478	24.24789568626178	24.666379729633675
17	23.992654618763858	25.80806749422539	25.494360835976472	24.70491705103428
18	24.700563947227614	24.895113427496177	26.480221549097436	23.92410107617877
19	25.57266157012281	24.848701601443107	25.51389404270776	24.06474278572632
20	25.781837679550097	24.379634273023605	25.12772235555396	24.710805691872338
21	26.544620504494038	24.222351697346113	24.675925070842855	24.557102727316995
22	25.690757862133314	24.480703779096288	25.033959311511687	24.794579047258715
23	24.33074268392473	24.24874711896129	25.657391746264864	25.763118450849117
24	24.8794562026535	24.86009083811171	25.31221053397474	24.948242425260048
25	24.855763741344145	24.49441749148687	25.51000418660153	25.139814580567453
26	24.67654880160037	25.163845558384658	25.760400673685247	24.39920496632973
27	25.152680920804066	24.88529690149107	24.99354908766468	24.968473090040185
28	24.496085327983916	24.894325124873223	25.66718459296143	24.942404954181434
29	24.623889607492934	24.800085971288052	25.510748369987734	25.06527605123128
30	24.54519148050157	25.08475449043479	25.709897566985013	24.66015646207863
31	25.200987600143964	24.675562381860487	24.949609667331448	25.1738403506641
32	24.68812757888044	24.969943379371642	24.80376440982678	25.53816463192114
33	24.48067936368984	24.634266066574014	25.455148932645177	25.429905637090965
34	25.14177482150813	24.674192168970063	25.516221547086253	24.667811462435548
35	25.39220377973117	24.74265868518192	25.589725937306724	24.275411597780185
36	24.65187326272472	25.05986358156056	25.266573452250746	25.021689703463974
37	25.350510772654534	24.914040972636958	24.977345325524862	24.758102929183643
38	24.754973574648677	24.868097139613845	25.351880896167007	25.025048389570465
39	25.27245687790249	24.607791390748865	24.880150317947983	25.239601413400663
40	25.486203501639103	24.883510975015238	25.37247103376209	24.257814489583573
41	24.682881506295352	25.00174909816499	25.620957834140718	24.694411561398944
42	25.641601130151574	25.31333553109904	25.16965986310793	23.87540347564146
43	24.847804051879553	24.57360787652161	25.50181146340385	25.07677660819499
44	24.64285474413825	25.06128726769607	25.031119379761655	25.264738608404024
45	24.656176399473434	25.220185909892805	25.156715950101642	24.966921740532115
46	24.537661514833676	24.849637147824062	25.207675921458705	25.405025415883557
47	24.85032135363116	24.720022432906973	25.637543999673134	24.792112213788737
48	25.3886616472	25.2441448884053	24.732795117549998	24.634398346844698
49	24.726533728253106	25.326972975756313	24.854143859154235	25.092349436836344
50	24.69840406299569	25.63193262071953	24.785773694185025	24.88388962209976
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	99.0
1	76.0
2	53.0
3	164.0
4	275.0
5	305.5
6	336.0
7	324.0
8	312.0
9	435.5
10	559.0
11	813.0
12	1067.0
13	1922.0
14	2777.0
15	3642.5
16	4508.0
17	4835.5
18	5163.0
19	5106.5
20	5050.0
21	5719.5
22	6389.0
23	7500.5
24	8612.0
25	10013.0
26	11414.0
27	13926.0
28	16438.0
29	19712.0
30	22986.0
31	26700.5
32	30415.0
33	35572.5
34	40730.0
35	46509.0
36	52288.0
37	60196.5
38	68105.0
39	72936.0
40	77767.0
41	84466.5
42	91166.0
43	94170.0
44	97174.0
45	106645.0
46	116116.0
47	123253.0
48	130390.0
49	135244.5
50	140099.0
51	136622.0
52	133145.0
53	131273.0
54	129401.0
55	128658.0
56	127915.0
57	123397.0
58	118879.0
59	108808.5
60	98738.0
61	86740.0
62	74742.0
63	64428.5
64	54115.0
65	45849.0
66	37583.0
67	32612.5
68	27642.0
69	24487.5
70	21333.0
71	16501.0
72	11669.0
73	9861.5
74	8054.0
75	6362.0
76	4670.0
77	4050.5
78	3431.0
79	2804.5
80	2178.0
81	1741.0
82	1304.0
83	1109.0
84	914.0
85	629.0
86	344.0
87	235.5
88	127.0
89	105.5
90	84.0
91	60.0
92	36.0
93	36.5
94	37.0
95	37.5
96	38.0
97	27.5
98	17.0
99	17.0
100	17.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008899082722850662
2	3.9178351610034355E-4
3	2.2387629491448205E-4
4	0.0
5	0.0
6	3.3581444237172305E-4
7	1.1193814745724102E-4
8	1.6790722118586153E-4
9	0.0011193814745724103
10	9.514742533865487E-4
11	0.0
12	1.1193814745724102E-4
13	4.477525898289641E-4
14	2.7984536864310256E-4
15	0.003693958866088954
16	7.835670322006871E-4
17	0.0
18	5.037216635575846E-4
19	0.0
20	7.275979584720667E-4
21	0.0
22	2.2387629491448205E-4
23	0.0010634124008437897
24	3.3581444237172305E-4
25	0.002742484612702405
26	0.006156598110148256
27	0.007387917732177908
28	0.004981247561847225
29	0.0032462062762599895
30	0.003917835161003436
31	0.008283422911835836
32	0.004085742382189297
33	0.004925278488118605
34	0.0036379897923603335
35	0.003693958866088954
36	0.007779701248278251
37	0.005261092930490328
38	0.008899082722850662
39	0.004925278488118605
40	0.0029103918338882666
41	0.003693958866088954
42	0.0021268248016875794
43	0.0013432577694868922
44	0.001846979433044477
45	0.0016231031381299947
46	0.0012872886957582719
47	0.0022387629491448205
48	0.003190237202531369
49	5.037216635575846E-4
50	0.0021827938754162
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1786701.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.88032076936583
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.98682104997202	43.02048255286201
2	13.6199921777742	15.221790635405515
3	4.172275504529422	6.994442805938152
4	1.8219805755011271	4.07251435978227
5	0.9116039741559806	2.5470361245232436
6	0.5744691191272868	1.9260911189356689
7	0.3680920610102345	1.4398371709336215
8	0.252219008233504	1.127526326737562
9	0.18518722147396957	0.931348920451771
>10	0.9726610786193259	9.576543436706931
>50	0.0683393796332064	2.64442460434794
>100	0.05768657421413645	6.605476599618888
>500	0.006756307856160917	2.558625729527983
>1k	0.0019159678995083198	1.3338596142284473
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	2.2387629491448205E-4
13	0.0	0.0	0.0	0.0	2.2387629491448205E-4
14	0.0	0.0	0.0	5.596907372862051E-5	2.2387629491448205E-4
15	0.0	0.0	0.0	5.596907372862051E-5	4.477525898289641E-4
16	0.0	0.0	0.0	5.596907372862051E-5	5.037216635575846E-4
17	0.0	0.0	0.0	5.596907372862051E-5	5.037216635575846E-4
18	0.0	0.0	0.0	1.6790722118586153E-4	5.037216635575846E-4
19	0.0	0.0	0.0	1.6790722118586153E-4	5.037216635575846E-4
20	0.0	0.0	0.0	2.2387629491448205E-4	6.156598110148257E-4
21	0.0	0.0	0.0	2.2387629491448205E-4	6.156598110148257E-4
22	0.0	0.0	0.0	3.917835161003436E-4	6.716288847434461E-4
23	0.0	0.0	0.0	0.0011193814745724103	8.395361059293077E-4
24	0.0	0.0	0.0	0.0020148866542303383	8.955051796579282E-4
25	0.0	0.0	0.0	0.0027984536864310255	8.955051796579282E-4
26	0.0	0.0	0.0	0.0033021753499886102	8.955051796579282E-4
27	0.0	0.0	0.0	0.003973804234732057	8.955051796579282E-4
28	0.0	0.0	0.0	0.006772257921163082	8.955051796579282E-4
29	0.0	0.0	0.0	0.013376608621140302	8.955051796579282E-4
30	0.0	0.0	0.0	0.023451041892291996	9.514742533865487E-4
31	0.0	0.0	0.0	0.048525186922713986	9.514742533865487E-4
32	0.0	0.0	0.0	0.0730956102895784	0.0010634124008437897
33	0.0	0.0	0.0	0.09816975532000038	0.0010634124008437897
34	0.0	0.0	0.0	0.1274415808800689	0.0011753505483010308
35	0.0	0.0	0.0	0.16214240659181361	0.0011753505483010308
36	0.0	0.0	0.0	0.21133922239927105	0.0011753505483010308
37	0.0	0.0	0.0	0.2806289356753033	0.0011753505483010308
38	0.0	0.0	0.0	0.37739946415208814	0.0012313196220296513
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACCG	65	1.8154315E-5	23.689928	5
AATCGAT	65	4.930258E-4	20.309631	38
GTCCTAC	555	0.0	19.02618	1
GTATCAA	3295	0.0	18.627396	1
CGTATTA	60	0.007416556	18.332003	15
TAGGACG	505	0.0	18.29519	4
ACGACGG	470	0.0	18.253485	6
CGACGGT	460	0.0	18.172087	7
TACGACG	475	0.0	18.061342	5
GTAGGAC	610	0.0	17.670355	3
ACGGTAT	490	0.0	17.05951	9
GACGGTA	495	0.0	16.88719	8
TATGGCG	540	0.0	16.702494	16
CTACGAC	515	0.0	16.658518	4
TCCTACA	695	0.0	16.458778	2
TGGCGAG	550	0.0	16.398354	18
ATGGCGA	590	0.0	16.032288	17
AGGACGT	510	0.0	15.95918	5
TGTAGGA	650	0.0	15.906094	2
TTAGGAC	410	0.0	15.559412	3
>>END_MODULE