FastQCFastQC Report
Thu 2 Feb 2017
SRR4064105_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064105_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATATGGAACACCAGCAACGAGCC11.639344262295082No Hit
CATTATCTGCGTCCTCTTCGCTCGG11.639344262295082No Hit
CCATCGACCTTCACTGTCATGGTGC11.639344262295082No Hit
CTCAGGCTCATCATATATATGGTCA11.639344262295082No Hit
CAACAGCATTAATCCCACAAGAACA11.639344262295082No Hit
CTCCGAGGAGGGTCACCCATGGTCA11.639344262295082No Hit
CCCTTTACCCACTGAGCTGTCTCCT11.639344262295082No Hit
CTCCCGTGGAGCAGAAGGGCAGACG11.639344262295082No Hit
CTCCTGGTGGTGCCCTTCCGTCACT11.639344262295082No Hit
GTGTGTAGCAGACGTAGTAGCCACT11.639344262295082No Hit
CCCTAGATGTGCTCAAGACCTAACC11.639344262295082No Hit
CAATTTAACAATTAACGTTAATTTA11.639344262295082No Hit
AGTTAGCACCTCCAGTCACCATACA11.639344262295082No Hit
GGACTCTTTCGAGGCCCTGTAATTG11.639344262295082No Hit
ACACTGGTCTAGGACCCGAGACGAG11.639344262295082No Hit
CCTATCAGACTGGCCCAGGGCTGCT11.639344262295082No Hit
GGCGCCACTCCTGGGGGTGCCCTTC11.639344262295082No Hit
GTTCCGTCTTGCTAAGGTCAAAGAA11.639344262295082No Hit
CTTCTTACACTGTCTCTAACACGAC11.639344262295082No Hit
TGTCTGGTGCATGCTGTGGTGCCTG11.639344262295082No Hit
GAATAAACCATCACTACAACCCGAA11.639344262295082No Hit
GATATACACTGTTCTACAAATCCCG11.639344262295082No Hit
GGGCTATAGAGAAGCCCTGTCTCGA11.639344262295082No Hit
TATCAACGCAGAGTACATGGGTACC11.639344262295082No Hit
AGGTTATCTAGAGTCACCAAAGCCG11.639344262295082No Hit
ACTCCCGCCCACGGTTTTCTTGTGT11.639344262295082No Hit
CCATAAACGATGCCGACTGGCGATG11.639344262295082No Hit
CTTGTATACTCTCCGAGTCTTCTTT11.639344262295082No Hit
CTCATGAGGAGAGGGAGCATGCCCA11.639344262295082No Hit
TCTTCGAACCTCCGACTTTCGTTCT11.639344262295082No Hit
GGCTCAACGCAGAGTAGATGGGGAA11.639344262295082No Hit
GCGCATAAGACAGACAACGAGAAGA11.639344262295082No Hit
ATCCAGGGCCATGGGGCGCACCAGG11.639344262295082No Hit
GTGCTCTGCACCAACTCTTAGCCAA11.639344262295082No Hit
ACCCTCCACGAGGACCTACGGGCGG11.639344262295082No Hit
TTAAATAGCTATGCAATGAAAGTTT11.639344262295082No Hit
CCATTGGATTGGGCCAGCTTCCGCG11.639344262295082No Hit
CTAATAAAACACTCATAATCAATAT11.639344262295082No Hit
CAGCAAACCAGTGACACAGGTGACC11.639344262295082No Hit
TATGTAACCTCCCCACCTTTTTCTC11.639344262295082No Hit
GCGGCAGGCGGGCAGATCTGGAGGG11.639344262295082No Hit
GGGGCGGGGCAGGCCGGGCGCCGCC11.639344262295082No Hit
CCATATTACACTTCCATCACTCTTA11.639344262295082No Hit
GAAAAAAATAAACCACAACAGCCAC11.639344262295082No Hit
GTTCTTATACCGCGCCACTACTCGC11.639344262295082No Hit
GTTCGGGGGTGGTGGGTTGAGTGGT11.639344262295082No Hit
TCCTAGCTCTTCTTAGGAAACAACA11.639344262295082No Hit
CTTCTAACTAAAAGAATTACAGCTA11.639344262295082No Hit
GATGAAAGCTCAGGCAGCAGAGCCC11.639344262295082No Hit
CATTTCACCATCTGATGTTTTCTTT11.639344262295082No Hit
GTGTAGATCTCGGAGGGCGCCGTCT11.639344262295082No Hit
ATCCACGCAGAGTACCTGGGCTCTT11.639344262295082No Hit
TCTCAGCACGTTCCGTCTTCTGCTC11.639344262295082No Hit
CTCCAGTGCTTCAGAAGAGAGTTGC11.639344262295082No Hit
ATTCTCAGAGCTTCACCTTCGATGA11.639344262295082No Hit
CTCCACTCCTGCTGCTGCCCTTCCG11.639344262295082No Hit
CAGTCATGTTTTATAATATAAAATA11.639344262295082No Hit
GCCCGCCAAGAGGTTCCGTATATTC11.639344262295082No Hit
CTGTCATCCAAATAGGACCTTAGGT11.639344262295082No Hit
GTATCAACGCAGAGTACATGGGCAT11.639344262295082No Hit
AGACCGAAAGAATCAGTGATTGATT11.639344262295082No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTACGG50.019.015
GAGGACC50.019.010
GGACCTA50.019.012
AGGACCT50.019.011
CCACGAG50.019.06
TACGGGC50.019.017
CTCCACG50.019.04
CACGAGG50.019.07
CGAGGAC50.019.09
CGGGCGG50.019.019
CCTCCAC50.019.03
ACGAGGA50.019.08
ACCCTCC50.019.01
ACGGGCG50.019.018
TCCACGA50.019.05
CTACGGG50.019.016
ACCTACG50.019.014
GACCTAC50.019.013
CCCTCCA50.019.02
Produced by FastQC (version 0.11.5)