##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064105_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	61
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.983606557377048	NaN	NaN	NaN	NaN	NaN
2	25.60655737704918	NaN	NaN	NaN	NaN	NaN
3	27.62295081967213	NaN	NaN	NaN	NaN	NaN
4	25.737704918032787	NaN	NaN	NaN	NaN	NaN
5	27.0	NaN	NaN	NaN	NaN	NaN
6	29.57377049180328	NaN	NaN	NaN	NaN	NaN
7	29.311475409836067	NaN	NaN	NaN	NaN	NaN
8	28.18032786885246	NaN	NaN	NaN	NaN	NaN
9	28.34426229508197	NaN	NaN	NaN	NaN	NaN
10	27.327868852459016	NaN	NaN	NaN	NaN	NaN
11	28.098360655737704	NaN	NaN	NaN	NaN	NaN
12	28.40983606557377	NaN	NaN	NaN	NaN	NaN
13	26.327868852459016	NaN	NaN	NaN	NaN	NaN
14	28.049180327868854	NaN	NaN	NaN	NaN	NaN
15	25.508196721311474	NaN	NaN	NaN	NaN	NaN
16	27.39344262295082	NaN	NaN	NaN	NaN	NaN
17	27.34426229508197	NaN	NaN	NaN	NaN	NaN
18	29.21311475409836	NaN	NaN	NaN	NaN	NaN
19	27.40983606557377	NaN	NaN	NaN	NaN	NaN
20	27.049180327868854	NaN	NaN	NaN	NaN	NaN
21	28.098360655737704	NaN	NaN	NaN	NaN	NaN
22	29.147540983606557	NaN	NaN	NaN	NaN	NaN
23	28.098360655737704	NaN	NaN	NaN	NaN	NaN
24	28.229508196721312	NaN	NaN	NaN	NaN	NaN
25	26.475409836065573	NaN	NaN	NaN	NaN	NaN
>>END_MODULE
>>Per sequence quality scores	warn
#Quality	Count
17	1.0
18	0.0
19	0.0
20	2.0
21	2.0
22	0.0
23	10.0
24	2.0
25	4.0
26	5.0
27	4.0
28	8.0
29	4.0
30	4.0
31	4.0
32	3.0
33	3.0
34	4.0
35	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.147540983606557	14.754098360655737	11.475409836065573	42.62295081967213
2	14.754098360655737	21.311475409836063	37.704918032786885	26.229508196721312
3	18.0327868852459	24.59016393442623	31.147540983606557	26.229508196721312
4	9.836065573770492	11.475409836065573	34.42622950819672	44.26229508196721
5	8.19672131147541	34.42622950819672	36.0655737704918	21.311475409836063
6	34.42622950819672	31.147540983606557	16.39344262295082	18.0327868852459
7	19.672131147540984	44.26229508196721	19.672131147540984	16.39344262295082
8	27.86885245901639	26.229508196721312	16.39344262295082	29.508196721311474
9	21.311475409836063	16.39344262295082	14.754098360655737	47.540983606557376
10	14.754098360655737	31.147540983606557	27.86885245901639	26.229508196721312
11	27.86885245901639	31.147540983606557	22.950819672131146	18.0327868852459
12	27.86885245901639	22.950819672131146	21.311475409836063	27.86885245901639
13	31.147540983606557	18.0327868852459	29.508196721311474	21.311475409836063
14	31.147540983606557	19.672131147540984	9.836065573770492	39.34426229508197
15	18.0327868852459	34.42622950819672	18.0327868852459	29.508196721311474
16	21.311475409836063	34.42622950819672	14.754098360655737	29.508196721311474
17	27.86885245901639	14.754098360655737	26.229508196721312	31.147540983606557
18	16.39344262295082	24.59016393442623	34.42622950819672	24.59016393442623
19	24.59016393442623	29.508196721311474	24.59016393442623	21.311475409836063
20	24.59016393442623	26.229508196721312	24.59016393442623	24.59016393442623
21	31.147540983606557	21.311475409836063	22.950819672131146	24.59016393442623
22	27.86885245901639	21.311475409836063	22.950819672131146	27.86885245901639
23	19.672131147540984	22.950819672131146	27.86885245901639	29.508196721311474
24	21.311475409836063	14.754098360655737	26.229508196721312	37.704918032786885
25	21.311475409836063	27.86885245901639	26.229508196721312	24.59016393442623
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	1.0
15	2.0
16	2.0
17	2.0
18	1.5
19	1.0
20	1.0
21	1.0
22	1.0
23	1.0
24	1.0
25	1.0
26	1.0
27	1.0
28	1.0
29	1.0
30	1.0
31	1.0
32	1.0
33	1.0
34	2.0
35	3.0
36	3.0
37	3.0
38	4.5
39	6.0
40	6.0
41	6.0
42	5.5
43	5.0
44	5.0
45	5.0
46	5.5
47	6.0
48	6.0
49	6.0
50	6.0
51	6.0
52	6.0
53	6.0
54	9.0
55	12.0
56	12.0
57	12.0
58	8.0
59	4.0
60	4.0
61	4.0
62	5.0
63	6.0
64	6.0
65	6.0
66	4.0
67	2.0
68	2.0
69	2.0
70	2.0
71	2.0
72	2.0
73	2.0
74	2.0
75	2.0
76	2.0
77	2.0
78	1.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.5
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	61.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	100.0
#Duplication Level	Percentage of deduplicated	Percentage of total
1	100.0	100.0
2	0.0	0.0
3	0.0	0.0
4	0.0	0.0
5	0.0	0.0
6	0.0	0.0
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CAATATGGAACACCAGCAACGAGCC	1	1.639344262295082	No Hit
CATTATCTGCGTCCTCTTCGCTCGG	1	1.639344262295082	No Hit
CCATCGACCTTCACTGTCATGGTGC	1	1.639344262295082	No Hit
CTCAGGCTCATCATATATATGGTCA	1	1.639344262295082	No Hit
CAACAGCATTAATCCCACAAGAACA	1	1.639344262295082	No Hit
CTCCGAGGAGGGTCACCCATGGTCA	1	1.639344262295082	No Hit
CCCTTTACCCACTGAGCTGTCTCCT	1	1.639344262295082	No Hit
CTCCCGTGGAGCAGAAGGGCAGACG	1	1.639344262295082	No Hit
CTCCTGGTGGTGCCCTTCCGTCACT	1	1.639344262295082	No Hit
GTGTGTAGCAGACGTAGTAGCCACT	1	1.639344262295082	No Hit
CCCTAGATGTGCTCAAGACCTAACC	1	1.639344262295082	No Hit
CAATTTAACAATTAACGTTAATTTA	1	1.639344262295082	No Hit
AGTTAGCACCTCCAGTCACCATACA	1	1.639344262295082	No Hit
GGACTCTTTCGAGGCCCTGTAATTG	1	1.639344262295082	No Hit
ACACTGGTCTAGGACCCGAGACGAG	1	1.639344262295082	No Hit
CCTATCAGACTGGCCCAGGGCTGCT	1	1.639344262295082	No Hit
GGCGCCACTCCTGGGGGTGCCCTTC	1	1.639344262295082	No Hit
GTTCCGTCTTGCTAAGGTCAAAGAA	1	1.639344262295082	No Hit
CTTCTTACACTGTCTCTAACACGAC	1	1.639344262295082	No Hit
TGTCTGGTGCATGCTGTGGTGCCTG	1	1.639344262295082	No Hit
GAATAAACCATCACTACAACCCGAA	1	1.639344262295082	No Hit
GATATACACTGTTCTACAAATCCCG	1	1.639344262295082	No Hit
GGGCTATAGAGAAGCCCTGTCTCGA	1	1.639344262295082	No Hit
TATCAACGCAGAGTACATGGGTACC	1	1.639344262295082	No Hit
AGGTTATCTAGAGTCACCAAAGCCG	1	1.639344262295082	No Hit
ACTCCCGCCCACGGTTTTCTTGTGT	1	1.639344262295082	No Hit
CCATAAACGATGCCGACTGGCGATG	1	1.639344262295082	No Hit
CTTGTATACTCTCCGAGTCTTCTTT	1	1.639344262295082	No Hit
CTCATGAGGAGAGGGAGCATGCCCA	1	1.639344262295082	No Hit
TCTTCGAACCTCCGACTTTCGTTCT	1	1.639344262295082	No Hit
GGCTCAACGCAGAGTAGATGGGGAA	1	1.639344262295082	No Hit
GCGCATAAGACAGACAACGAGAAGA	1	1.639344262295082	No Hit
ATCCAGGGCCATGGGGCGCACCAGG	1	1.639344262295082	No Hit
GTGCTCTGCACCAACTCTTAGCCAA	1	1.639344262295082	No Hit
ACCCTCCACGAGGACCTACGGGCGG	1	1.639344262295082	No Hit
TTAAATAGCTATGCAATGAAAGTTT	1	1.639344262295082	No Hit
CCATTGGATTGGGCCAGCTTCCGCG	1	1.639344262295082	No Hit
CTAATAAAACACTCATAATCAATAT	1	1.639344262295082	No Hit
CAGCAAACCAGTGACACAGGTGACC	1	1.639344262295082	No Hit
TATGTAACCTCCCCACCTTTTTCTC	1	1.639344262295082	No Hit
GCGGCAGGCGGGCAGATCTGGAGGG	1	1.639344262295082	No Hit
GGGGCGGGGCAGGCCGGGCGCCGCC	1	1.639344262295082	No Hit
CCATATTACACTTCCATCACTCTTA	1	1.639344262295082	No Hit
GAAAAAAATAAACCACAACAGCCAC	1	1.639344262295082	No Hit
GTTCTTATACCGCGCCACTACTCGC	1	1.639344262295082	No Hit
GTTCGGGGGTGGTGGGTTGAGTGGT	1	1.639344262295082	No Hit
TCCTAGCTCTTCTTAGGAAACAACA	1	1.639344262295082	No Hit
CTTCTAACTAAAAGAATTACAGCTA	1	1.639344262295082	No Hit
GATGAAAGCTCAGGCAGCAGAGCCC	1	1.639344262295082	No Hit
CATTTCACCATCTGATGTTTTCTTT	1	1.639344262295082	No Hit
GTGTAGATCTCGGAGGGCGCCGTCT	1	1.639344262295082	No Hit
ATCCACGCAGAGTACCTGGGCTCTT	1	1.639344262295082	No Hit
TCTCAGCACGTTCCGTCTTCTGCTC	1	1.639344262295082	No Hit
CTCCAGTGCTTCAGAAGAGAGTTGC	1	1.639344262295082	No Hit
ATTCTCAGAGCTTCACCTTCGATGA	1	1.639344262295082	No Hit
CTCCACTCCTGCTGCTGCCCTTCCG	1	1.639344262295082	No Hit
CAGTCATGTTTTATAATATAAAATA	1	1.639344262295082	No Hit
GCCCGCCAAGAGGTTCCGTATATTC	1	1.639344262295082	No Hit
CTGTCATCCAAATAGGACCTTAGGT	1	1.639344262295082	No Hit
GTATCAACGCAGAGTACATGGGCAT	1	1.639344262295082	No Hit
AGACCGAAAGAATCAGTGATTGATT	1	1.639344262295082	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTACGG	5	0.0	19.0	15
GAGGACC	5	0.0	19.0	10
GGACCTA	5	0.0	19.0	12
AGGACCT	5	0.0	19.0	11
CCACGAG	5	0.0	19.0	6
TACGGGC	5	0.0	19.0	17
CTCCACG	5	0.0	19.0	4
CACGAGG	5	0.0	19.0	7
CGAGGAC	5	0.0	19.0	9
CGGGCGG	5	0.0	19.0	19
CCTCCAC	5	0.0	19.0	3
ACGAGGA	5	0.0	19.0	8
ACCCTCC	5	0.0	19.0	1
ACGGGCG	5	0.0	19.0	18
TCCACGA	5	0.0	19.0	5
CTACGGG	5	0.0	19.0	16
ACCTACG	5	0.0	19.0	14
GACCTAC	5	0.0	19.0	13
CCCTCCA	5	0.0	19.0	2
>>END_MODULE