FastQCFastQC Report
Thu 2 Feb 2017
SRR4064105_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064105_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61
Sequences flagged as poor quality0
Sequence length50
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23.278688524590164No Hit
GTGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11.639344262295082No Hit
GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA11.639344262295082No Hit
GGGCAGCAGTGGTGGCAGCAGCTGCAGGGGCAGCCGCCGCAAATGCAGAT11.639344262295082No Hit
CACGCACCCCGGCGAGCCCGAGAAACCGCCGCGCAGCAGCGCCAACGGCG11.639344262295082No Hit
TATTAGATGAGTGAGTTACACTGAAAAAACACTTGCGTTGGAAACGGGAT11.639344262295082No Hit
GCGTGGTCCTGGCTGCCCACTCTGGCTCCTTCGACAATCGGTATTACCAG11.639344262295082No Hit
AAATGTGAAGTCTGTTGGTGGAGAGATAATGAGGTCCCATCATAAAGGCA11.639344262295082No Hit
CTGGCGCACATCCGTGCGAAGGGCTGTTAGCAGGAAAAGGAGCCTCAAGT11.639344262295082No Hit
TCCTGTGCCTCAGCCTCCTAGCTGTTGGCACTTGCCAGGACGATGCCGAG11.639344262295082No Hit
ACACAGGAAGTGGTCTGTCAAACACAAATCTGGAATCCAAACACTCCTGG11.639344262295082No Hit
CACACACATCCCCCAGCTCAGTTAGGAGTGTAGTGAGGGTTGCTGTGGTA11.639344262295082No Hit
GTGTGATGCCACATCCCAGCTCTCCATTAGAGCCTTAAAGGCGTTGTTAT11.639344262295082No Hit
CCACCGCTGCTGCCGCCGCCGCCCAGAGACCGCCGAGCCGCGTCCGTGTG11.639344262295082No Hit
GCCACTCACAGAGGCCTCTTATGTCAACCTGCCCACCATTGCTCTCTGTA11.639344262295082No Hit
CATTAAGGGTGGTTGTGAGCCACCCTGTGGTTGCTGGGATTTGAACTCAG11.639344262295082No Hit
CCATGAACCCCTTCTGTGAGATTGCAGTGGAAGAGGCTGTGCGGCTAAAG11.639344262295082No Hit
GCGAGGCTCTCCGCCGCCGCCTCCTCCTCCTCTCTCGCGCTCTCTGTCCC11.639344262295082No Hit
TCCCGAAACTCCCTGAATGCCTCAACCCGCCGGCGACCAGCAAGGATGTC11.639344262295082No Hit
GTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGCCGGACCAG11.639344262295082No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC11.639344262295082No Hit
GTGGGTTGTTCCTCCACACCCCCTCCTTTTTTTACGGTCAATATATTTGT11.639344262295082No Hit
ACGTGGTATATGGCAAGAAAAGTGAAAATCATGGAAAATGAGAAAAATCC11.639344262295082No Hit
CCGCCGAGCCGCGTCCGTGTGCCGCCACCCACTCCGGACACAGAATATCC11.639344262295082No Hit
GGCCTCCGAGCCGCGCACCCCGCTGTCCAGCAAGGCCAGGATGAAAAGGC11.639344262295082No Hit
ATATTATTGCTTCCCACTTTTCAACTGGTAAATAGTTGCCACTGATGAAA11.639344262295082No Hit
GGCTGCAGCCCAGCATGTTAACAGACACGTAGGGAGAGCAGTAACATTAA11.639344262295082No Hit
GGCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCG11.639344262295082No Hit
CGGTAGATGTCTGCCAGGGTCAAGGAAGAAGGGTCCTCTGGTCCCGCAAC11.639344262295082No Hit
CCTGGATACCGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTG11.639344262295082No Hit
GGTGTAGATGGCGAGGGGGCTGAGCAGGGACTGCCATAGTACTCAGAGCC11.639344262295082No Hit
TCAGAAGACTGTGCATTCAAGATAAGACTCCAGTGACCCCAGGCTTGGTA11.639344262295082No Hit
CCTCGCGGCGGACCGCCCGCCCGCTCCCAAGATCCAACTACGAGCTTTTT11.639344262295082No Hit
TCCCCACACACATCGACTGCGCACGCCGAGTCCCTTAGCAGCAAGATTAA11.639344262295082No Hit
GGCGGGCGGTCCGCCGCGCGGCGAGTCACCGCCCGTGCCCGCCCCTTGCC11.639344262295082No Hit
GTAAGTTAACTTAGGCACACCGGTTTGTATTTTAGAAAAAAATAATTATC11.639344262295082No Hit
TCCGTCGCCGCCGCGTCCTTCGCCGATCGCAAGGGAAGAAGAAATCGCCG11.639344262295082No Hit
GCTGGATGTACAGCCATGCTGGACACCCACTTGCAGTTGGGCATCAGCTT11.639344262295082No Hit
GATCATAGTCAGCTCCAGTTTTTTATATTGTCCAGGACCACTTGCCCAGG11.639344262295082No Hit
AGTGGGGATCATTCTTGTCAGTAGCCAGTTTGTGCAGTTCCAGTAGTGAC11.639344262295082No Hit
CCTGTCCGCCAGACCCTGACCTCCGAGCCCTGCACTGGACGGATAGGTGT11.639344262295082No Hit
AGGGCCGGCCGGGGGCCTTCGTATTGCGCCGCTAGAGGTGAAATTCTTGG11.639344262295082No Hit
GGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCGAATG11.639344262295082No Hit
CCCGTGCACAGCCTGCGGTTCGTGCACTGGGCCAACTGTGCCACATCCCC11.639344262295082No Hit
GGGTGGCGGAGGTCCGTTTTGTGACTGGATATAAATTCTTGAGATTCCTC11.639344262295082No Hit
CTCCGGGAGTCCTGGACGTGAGGCCGGAGGCCCCAGTGTCTGCGAGCCCA11.639344262295082No Hit
CTCGGCGCCCCCTCGATGCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGC11.639344262295082No Hit
ATCCAGGGCCTCAGATCCAGGGTCCTGCATCGGACCTCAGGACAGGTGCC11.639344262295082No Hit
ACTCGGGCCCATCCGGCGGGGGCCGGGGCCCCGAGGCTCGGAGATGGGAC11.639344262295082No Hit
GTCTCACGACGGTCTAATCCAGCTCACGTTCCCTATTAGTGGGTGAACCA11.639344262295082No Hit
CCCCACGAGCATCCGCGTGCACTTCCAGGCCGGCCGCTTCCACTTGGACG11.639344262295082No Hit
GTATTCCTTTAAGGAAGACAAGTGCAATAATGCCTCCCCTACGTGACCTT11.639344262295082No Hit
GTCTTAAAAAAAAAAAACAAAAACAAAAACAAAACTAAAAACAAAAGCAA11.639344262295082No Hit
GGATGTGGCTGCCTCCGAGTTCTACAGGTCTGGCAAGTATGACCTGGACT11.639344262295082No Hit
CCTCGGGACATGTCCCTCCAGCCCTGTGCCTTTCGTATCAGCAGCTGCAA11.639344262295082No Hit
GTCTGCCTCCTCACCCGAGTACAGGGTCCATCCACTAACGCCCTGTTCTG11.639344262295082No Hit
TATTCTGTGTCTGTATTCTTTGACTTTTCATTACAGCCACCGACGATCCT11.639344262295082No Hit
CCACCTGCCTCTGCCTCCTAGATGCTGGGATTAAAGGTGTGCAGCACCAT11.639344262295082No Hit
GCACTGAGCGCTCCCGCCGAGACCTGCCCGCCACCTCTGCCATGGCCGAA11.639344262295082No Hit
CCTGAGGCCCTGGGTGGTGGGGCTGTCTCAGACGTCTCATTGTCTTATTT11.639344262295082No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTGCT50.044.05
CCGTGTG50.044.044
CCCAGAG50.044.022
GCCCAGA50.044.021
CGCCCAG50.044.020
TGCCGCC50.044.011
GTCCGTG50.044.042
CCGCCGA50.044.030
CCGCTGC50.044.04
CCGCCCA50.044.019
CACCGCT50.044.02
ACCGCTG50.044.03
CCAGAGA50.044.023
TGCTGCC50.044.08
ACCGCCG50.044.029
GAGCCGC50.044.035
CAGAGAC50.044.024
CCACCGC50.044.01
GCTGCTG50.044.06
GCTGCCG50.044.09
Produced by FastQC (version 0.11.5)