##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064105_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	61
Sequences flagged as poor quality	0
Sequence length	50
%GC	59
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.21311475409836	NaN	NaN	NaN	NaN	NaN
2	29.21311475409836	NaN	NaN	NaN	NaN	NaN
3	29.39344262295082	NaN	NaN	NaN	NaN	NaN
4	30.16393442622951	NaN	NaN	NaN	NaN	NaN
5	29.508196721311474	NaN	NaN	NaN	NaN	NaN
6	34.14754098360656	NaN	NaN	NaN	NaN	NaN
7	33.540983606557376	NaN	NaN	NaN	NaN	NaN
8	32.22950819672131	NaN	NaN	NaN	NaN	NaN
9	33.049180327868854	NaN	NaN	NaN	NaN	NaN
10	32.50819672131148	NaN	NaN	NaN	NaN	NaN
11	33.90163934426229	NaN	NaN	NaN	NaN	NaN
12	34.721311475409834	NaN	NaN	NaN	NaN	NaN
13	32.91803278688525	NaN	NaN	NaN	NaN	NaN
14	33.442622950819676	NaN	NaN	NaN	NaN	NaN
15	34.114754098360656	NaN	NaN	NaN	NaN	NaN
16	32.377049180327866	NaN	NaN	NaN	NaN	NaN
17	31.75409836065574	NaN	NaN	NaN	NaN	NaN
18	33.16393442622951	NaN	NaN	NaN	NaN	NaN
19	32.459016393442624	NaN	NaN	NaN	NaN	NaN
20	33.32786885245902	NaN	NaN	NaN	NaN	NaN
21	34.032786885245905	NaN	NaN	NaN	NaN	NaN
22	33.0	NaN	NaN	NaN	NaN	NaN
23	33.01639344262295	NaN	NaN	NaN	NaN	NaN
24	33.721311475409834	NaN	NaN	NaN	NaN	NaN
25	33.19672131147541	NaN	NaN	NaN	NaN	NaN
26	33.42622950819672	NaN	NaN	NaN	NaN	NaN
27	31.75409836065574	NaN	NaN	NaN	NaN	NaN
28	31.540983606557376	NaN	NaN	NaN	NaN	NaN
29	32.39344262295082	NaN	NaN	NaN	NaN	NaN
30	31.901639344262296	NaN	NaN	NaN	NaN	NaN
31	31.0	NaN	NaN	NaN	NaN	NaN
32	30.852459016393443	NaN	NaN	NaN	NaN	NaN
33	31.360655737704917	NaN	NaN	NaN	NaN	NaN
34	29.39344262295082	NaN	NaN	NaN	NaN	NaN
35	31.704918032786885	NaN	NaN	NaN	NaN	NaN
36	31.147540983606557	NaN	NaN	NaN	NaN	NaN
37	32.40983606557377	NaN	NaN	NaN	NaN	NaN
38	33.049180327868854	NaN	NaN	NaN	NaN	NaN
39	30.80327868852459	NaN	NaN	NaN	NaN	NaN
40	32.540983606557376	NaN	NaN	NaN	NaN	NaN
41	30.885245901639344	NaN	NaN	NaN	NaN	NaN
42	32.47540983606557	NaN	NaN	NaN	NaN	NaN
43	32.42622950819672	NaN	NaN	NaN	NaN	NaN
44	31.0327868852459	NaN	NaN	NaN	NaN	NaN
45	29.721311475409838	NaN	NaN	NaN	NaN	NaN
46	30.60655737704918	NaN	NaN	NaN	NaN	NaN
47	32.622950819672134	NaN	NaN	NaN	NaN	NaN
48	31.147540983606557	NaN	NaN	NaN	NaN	NaN
49	30.524590163934427	NaN	NaN	NaN	NaN	NaN
50	30.540983606557376	NaN	NaN	NaN	NaN	NaN
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
25	1.0
26	0.0
27	3.0
28	4.0
29	9.0
30	3.0
31	8.0
32	7.0
33	8.0
34	11.0
35	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.90163934426229	13.114754098360656	11.475409836065573	29.508196721311474
2	24.59016393442623	11.475409836065573	24.59016393442623	39.34426229508197
3	24.59016393442623	19.672131147540984	24.59016393442623	31.147540983606557
4	29.508196721311474	9.836065573770492	34.42622950819672	26.229508196721312
5	45.90163934426229	18.0327868852459	18.0327868852459	18.0327868852459
6	44.26229508196721	24.59016393442623	14.754098360655737	16.39344262295082
7	40.98360655737705	14.754098360655737	13.114754098360656	31.147540983606557
8	37.704918032786885	32.78688524590164	13.114754098360656	16.39344262295082
9	22.950819672131146	11.475409836065573	13.114754098360656	52.459016393442624
10	19.672131147540984	18.0327868852459	24.59016393442623	37.704918032786885
11	34.42622950819672	14.754098360655737	21.311475409836063	29.508196721311474
12	27.86885245901639	14.754098360655737	21.311475409836063	36.0655737704918
13	40.98360655737705	11.475409836065573	22.950819672131146	24.59016393442623
14	27.86885245901639	8.19672131147541	16.39344262295082	47.540983606557376
15	36.0655737704918	14.754098360655737	13.114754098360656	36.0655737704918
16	24.59016393442623	22.950819672131146	22.950819672131146	29.508196721311474
17	31.147540983606557	16.39344262295082	19.672131147540984	32.78688524590164
18	36.0655737704918	13.114754098360656	22.950819672131146	27.86885245901639
19	34.42622950819672	14.754098360655737	22.950819672131146	27.86885245901639
20	19.672131147540984	29.508196721311474	21.311475409836063	29.508196721311474
21	40.98360655737705	24.59016393442623	13.114754098360656	21.311475409836063
22	32.78688524590164	16.39344262295082	21.311475409836063	29.508196721311474
23	21.311475409836063	24.59016393442623	27.86885245901639	26.229508196721312
24	29.508196721311474	22.950819672131146	18.0327868852459	29.508196721311474
25	26.229508196721312	19.672131147540984	19.672131147540984	34.42622950819672
26	26.229508196721312	29.508196721311474	21.311475409836063	22.950819672131146
27	32.78688524590164	26.229508196721312	19.672131147540984	21.311475409836063
28	39.34426229508197	14.754098360655737	22.950819672131146	22.950819672131146
29	24.59016393442623	31.147540983606557	19.672131147540984	24.59016393442623
30	31.147540983606557	18.0327868852459	19.672131147540984	31.147540983606557
31	22.950819672131146	21.311475409836063	32.78688524590164	22.950819672131146
32	27.86885245901639	19.672131147540984	21.311475409836063	31.147540983606557
33	44.26229508196721	11.475409836065573	21.311475409836063	22.950819672131146
34	22.950819672131146	29.508196721311474	9.836065573770492	37.704918032786885
35	36.0655737704918	24.59016393442623	11.475409836065573	27.86885245901639
36	29.508196721311474	24.59016393442623	21.311475409836063	24.59016393442623
37	32.78688524590164	22.950819672131146	19.672131147540984	24.59016393442623
38	22.950819672131146	22.950819672131146	22.950819672131146	31.147540983606557
39	32.78688524590164	22.950819672131146	18.0327868852459	26.229508196721312
40	32.78688524590164	22.950819672131146	22.950819672131146	21.311475409836063
41	40.98360655737705	22.950819672131146	11.475409836065573	24.59016393442623
42	31.147540983606557	26.229508196721312	13.114754098360656	29.508196721311474
43	24.59016393442623	32.78688524590164	19.672131147540984	22.950819672131146
44	21.311475409836063	27.86885245901639	26.229508196721312	24.59016393442623
45	24.59016393442623	26.229508196721312	22.950819672131146	26.229508196721312
46	29.508196721311474	22.950819672131146	26.229508196721312	21.311475409836063
47	31.147540983606557	19.672131147540984	29.508196721311474	19.672131147540984
48	36.0655737704918	18.0327868852459	19.672131147540984	26.229508196721312
49	18.0327868852459	31.147540983606557	22.950819672131146	27.86885245901639
50	31.147540983606557	21.311475409836063	21.311475409836063	26.229508196721312
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.5
18	1.0
19	0.5
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.5
28	1.0
29	0.5
30	0.0
31	0.0
32	0.0
33	1.0
34	2.0
35	1.0
36	0.0
37	1.0
38	2.0
39	1.5
40	1.0
41	1.0
42	1.0
43	1.5
44	2.0
45	2.0
46	2.0
47	3.5
48	5.0
49	3.0
50	1.0
51	1.5
52	2.0
53	3.0
54	4.0
55	4.5
56	5.0
57	3.0
58	1.0
59	3.0
60	5.0
61	4.5
62	4.0
63	3.0
64	2.0
65	2.5
66	3.0
67	2.5
68	2.0
69	3.0
70	4.0
71	3.5
72	3.0
73	2.0
74	1.0
75	0.5
76	0.0
77	0.5
78	1.0
79	1.5
80	2.0
81	1.0
82	0.0
83	0.0
84	0.0
85	0.5
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	61.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	98.36065573770492
#Duplication Level	Percentage of deduplicated	Percentage of total
1	98.33333333333333	96.72131147540983
2	1.6666666666666667	3.278688524590164
3	0.0	0.0
4	0.0	0.0
5	0.0	0.0
6	0.0	0.0
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	2	3.278688524590164	No Hit
GTGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	1	1.639344262295082	No Hit
GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA	1	1.639344262295082	No Hit
GGGCAGCAGTGGTGGCAGCAGCTGCAGGGGCAGCCGCCGCAAATGCAGAT	1	1.639344262295082	No Hit
CACGCACCCCGGCGAGCCCGAGAAACCGCCGCGCAGCAGCGCCAACGGCG	1	1.639344262295082	No Hit
TATTAGATGAGTGAGTTACACTGAAAAAACACTTGCGTTGGAAACGGGAT	1	1.639344262295082	No Hit
GCGTGGTCCTGGCTGCCCACTCTGGCTCCTTCGACAATCGGTATTACCAG	1	1.639344262295082	No Hit
AAATGTGAAGTCTGTTGGTGGAGAGATAATGAGGTCCCATCATAAAGGCA	1	1.639344262295082	No Hit
CTGGCGCACATCCGTGCGAAGGGCTGTTAGCAGGAAAAGGAGCCTCAAGT	1	1.639344262295082	No Hit
TCCTGTGCCTCAGCCTCCTAGCTGTTGGCACTTGCCAGGACGATGCCGAG	1	1.639344262295082	No Hit
ACACAGGAAGTGGTCTGTCAAACACAAATCTGGAATCCAAACACTCCTGG	1	1.639344262295082	No Hit
CACACACATCCCCCAGCTCAGTTAGGAGTGTAGTGAGGGTTGCTGTGGTA	1	1.639344262295082	No Hit
GTGTGATGCCACATCCCAGCTCTCCATTAGAGCCTTAAAGGCGTTGTTAT	1	1.639344262295082	No Hit
CCACCGCTGCTGCCGCCGCCGCCCAGAGACCGCCGAGCCGCGTCCGTGTG	1	1.639344262295082	No Hit
GCCACTCACAGAGGCCTCTTATGTCAACCTGCCCACCATTGCTCTCTGTA	1	1.639344262295082	No Hit
CATTAAGGGTGGTTGTGAGCCACCCTGTGGTTGCTGGGATTTGAACTCAG	1	1.639344262295082	No Hit
CCATGAACCCCTTCTGTGAGATTGCAGTGGAAGAGGCTGTGCGGCTAAAG	1	1.639344262295082	No Hit
GCGAGGCTCTCCGCCGCCGCCTCCTCCTCCTCTCTCGCGCTCTCTGTCCC	1	1.639344262295082	No Hit
TCCCGAAACTCCCTGAATGCCTCAACCCGCCGGCGACCAGCAAGGATGTC	1	1.639344262295082	No Hit
GTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGCCGGACCAG	1	1.639344262295082	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	1	1.639344262295082	No Hit
GTGGGTTGTTCCTCCACACCCCCTCCTTTTTTTACGGTCAATATATTTGT	1	1.639344262295082	No Hit
ACGTGGTATATGGCAAGAAAAGTGAAAATCATGGAAAATGAGAAAAATCC	1	1.639344262295082	No Hit
CCGCCGAGCCGCGTCCGTGTGCCGCCACCCACTCCGGACACAGAATATCC	1	1.639344262295082	No Hit
GGCCTCCGAGCCGCGCACCCCGCTGTCCAGCAAGGCCAGGATGAAAAGGC	1	1.639344262295082	No Hit
ATATTATTGCTTCCCACTTTTCAACTGGTAAATAGTTGCCACTGATGAAA	1	1.639344262295082	No Hit
GGCTGCAGCCCAGCATGTTAACAGACACGTAGGGAGAGCAGTAACATTAA	1	1.639344262295082	No Hit
GGCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCG	1	1.639344262295082	No Hit
CGGTAGATGTCTGCCAGGGTCAAGGAAGAAGGGTCCTCTGGTCCCGCAAC	1	1.639344262295082	No Hit
CCTGGATACCGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTG	1	1.639344262295082	No Hit
GGTGTAGATGGCGAGGGGGCTGAGCAGGGACTGCCATAGTACTCAGAGCC	1	1.639344262295082	No Hit
TCAGAAGACTGTGCATTCAAGATAAGACTCCAGTGACCCCAGGCTTGGTA	1	1.639344262295082	No Hit
CCTCGCGGCGGACCGCCCGCCCGCTCCCAAGATCCAACTACGAGCTTTTT	1	1.639344262295082	No Hit
TCCCCACACACATCGACTGCGCACGCCGAGTCCCTTAGCAGCAAGATTAA	1	1.639344262295082	No Hit
GGCGGGCGGTCCGCCGCGCGGCGAGTCACCGCCCGTGCCCGCCCCTTGCC	1	1.639344262295082	No Hit
GTAAGTTAACTTAGGCACACCGGTTTGTATTTTAGAAAAAAATAATTATC	1	1.639344262295082	No Hit
TCCGTCGCCGCCGCGTCCTTCGCCGATCGCAAGGGAAGAAGAAATCGCCG	1	1.639344262295082	No Hit
GCTGGATGTACAGCCATGCTGGACACCCACTTGCAGTTGGGCATCAGCTT	1	1.639344262295082	No Hit
GATCATAGTCAGCTCCAGTTTTTTATATTGTCCAGGACCACTTGCCCAGG	1	1.639344262295082	No Hit
AGTGGGGATCATTCTTGTCAGTAGCCAGTTTGTGCAGTTCCAGTAGTGAC	1	1.639344262295082	No Hit
CCTGTCCGCCAGACCCTGACCTCCGAGCCCTGCACTGGACGGATAGGTGT	1	1.639344262295082	No Hit
AGGGCCGGCCGGGGGCCTTCGTATTGCGCCGCTAGAGGTGAAATTCTTGG	1	1.639344262295082	No Hit
GGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCGAATG	1	1.639344262295082	No Hit
CCCGTGCACAGCCTGCGGTTCGTGCACTGGGCCAACTGTGCCACATCCCC	1	1.639344262295082	No Hit
GGGTGGCGGAGGTCCGTTTTGTGACTGGATATAAATTCTTGAGATTCCTC	1	1.639344262295082	No Hit
CTCCGGGAGTCCTGGACGTGAGGCCGGAGGCCCCAGTGTCTGCGAGCCCA	1	1.639344262295082	No Hit
CTCGGCGCCCCCTCGATGCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGC	1	1.639344262295082	No Hit
ATCCAGGGCCTCAGATCCAGGGTCCTGCATCGGACCTCAGGACAGGTGCC	1	1.639344262295082	No Hit
ACTCGGGCCCATCCGGCGGGGGCCGGGGCCCCGAGGCTCGGAGATGGGAC	1	1.639344262295082	No Hit
GTCTCACGACGGTCTAATCCAGCTCACGTTCCCTATTAGTGGGTGAACCA	1	1.639344262295082	No Hit
CCCCACGAGCATCCGCGTGCACTTCCAGGCCGGCCGCTTCCACTTGGACG	1	1.639344262295082	No Hit
GTATTCCTTTAAGGAAGACAAGTGCAATAATGCCTCCCCTACGTGACCTT	1	1.639344262295082	No Hit
GTCTTAAAAAAAAAAAACAAAAACAAAAACAAAACTAAAAACAAAAGCAA	1	1.639344262295082	No Hit
GGATGTGGCTGCCTCCGAGTTCTACAGGTCTGGCAAGTATGACCTGGACT	1	1.639344262295082	No Hit
CCTCGGGACATGTCCCTCCAGCCCTGTGCCTTTCGTATCAGCAGCTGCAA	1	1.639344262295082	No Hit
GTCTGCCTCCTCACCCGAGTACAGGGTCCATCCACTAACGCCCTGTTCTG	1	1.639344262295082	No Hit
TATTCTGTGTCTGTATTCTTTGACTTTTCATTACAGCCACCGACGATCCT	1	1.639344262295082	No Hit
CCACCTGCCTCTGCCTCCTAGATGCTGGGATTAAAGGTGTGCAGCACCAT	1	1.639344262295082	No Hit
GCACTGAGCGCTCCCGCCGAGACCTGCCCGCCACCTCTGCCATGGCCGAA	1	1.639344262295082	No Hit
CCTGAGGCCCTGGGTGGTGGGGCTGTCTCAGACGTCTCATTGTCTTATTT	1	1.639344262295082	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.0	0.0
30	0.0	0.0	0.0	0.0	0.0
31	0.0	0.0	0.0	0.0	0.0
32	0.0	0.0	0.0	0.0	0.0
33	0.0	0.0	0.0	0.0	0.0
34	0.0	0.0	0.0	0.0	0.0
35	0.0	0.0	0.0	0.0	0.0
36	0.0	0.0	0.0	0.0	0.0
37	0.0	0.0	0.0	0.0	0.0
38	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCTGCT	5	0.0	44.0	5
CCGTGTG	5	0.0	44.0	44
CCCAGAG	5	0.0	44.0	22
GCCCAGA	5	0.0	44.0	21
CGCCCAG	5	0.0	44.0	20
TGCCGCC	5	0.0	44.0	11
GTCCGTG	5	0.0	44.0	42
CCGCCGA	5	0.0	44.0	30
CCGCTGC	5	0.0	44.0	4
CCGCCCA	5	0.0	44.0	19
CACCGCT	5	0.0	44.0	2
ACCGCTG	5	0.0	44.0	3
CCAGAGA	5	0.0	44.0	23
TGCTGCC	5	0.0	44.0	8
ACCGCCG	5	0.0	44.0	29
GAGCCGC	5	0.0	44.0	35
CAGAGAC	5	0.0	44.0	24
CCACCGC	5	0.0	44.0	1
GCTGCTG	5	0.0	44.0	6
GCTGCCG	5	0.0	44.0	9
>>END_MODULE