FastQCFastQC Report
Thu 2 Feb 2017
SRR4064104_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064104_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2247083
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT35820.1594066618812033No Hit
TATCAACGCAGAGTACTTTTTTTTT24590.10943075978946928No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG24220.10778418064664279No Hit
GGGTAGGCACACGCTGAGCCAGTCA23070.10266643466218203No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGCGT2550.013.78575213
GCGTTAT1500.013.29546451
TAATTGC3850.013.07928713
CGTCTTA1102.741217E-912.9570515
GGTATCA10600.012.9012591
CGGCGTC2700.012.66883614
GTATCAA27300.012.5927731
GTTATTC2000.012.3446883
TAATACC1551.8189894E-1212.2544314
GTATAGA1900.011.9959071
AGGCCCG4050.011.96447810
ATCGCCA3850.011.84644716
CGAGCCG4100.011.81935915
AATTGCA4350.011.79512214
CGTTATT1701.8189894E-1211.7297252
CGCATCG3900.011.69352813
CGGTCGG3050.011.52604910
CGAACGA2400.011.48138716
GGTCGGC3100.011.34014611
CGACCAT3400.011.17788610
Produced by FastQC (version 0.11.5)