##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064104_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2247083
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.245382124291805	32.0	32.0	32.0	32.0	32.0
2	30.801266352867252	32.0	32.0	32.0	32.0	32.0
3	30.817345865729035	32.0	32.0	32.0	32.0	32.0
4	30.85880138828873	32.0	32.0	32.0	32.0	32.0
5	30.745348525176862	32.0	32.0	32.0	32.0	32.0
6	34.40652926482911	36.0	36.0	36.0	32.0	36.0
7	34.35445063666985	36.0	36.0	36.0	32.0	36.0
8	34.31184873900964	36.0	36.0	36.0	32.0	36.0
9	34.47028347417518	36.0	36.0	36.0	32.0	36.0
10	34.13210192947924	36.0	36.0	36.0	32.0	36.0
11	34.43989474354085	36.0	36.0	36.0	32.0	36.0
12	34.23042896056799	36.0	36.0	36.0	32.0	36.0
13	34.330810655414155	36.0	36.0	36.0	32.0	36.0
14	34.2140837699364	36.0	36.0	36.0	32.0	36.0
15	34.134307900509235	36.0	36.0	36.0	32.0	36.0
16	34.13958674423686	36.0	36.0	36.0	32.0	36.0
17	34.06209694968989	36.0	36.0	36.0	32.0	36.0
18	34.05999288855819	36.0	36.0	36.0	32.0	36.0
19	34.08547258823995	36.0	36.0	36.0	32.0	36.0
20	34.06246765250772	36.0	36.0	36.0	32.0	36.0
21	34.05087262019249	36.0	36.0	36.0	32.0	36.0
22	34.02859173426171	36.0	36.0	36.0	32.0	36.0
23	33.96328484528609	36.0	36.0	36.0	32.0	36.0
24	33.95007794549645	36.0	36.0	36.0	32.0	36.0
25	33.53927202510989	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	8.0
4	17.0
5	27.0
6	91.0
7	35.0
8	148.0
9	135.0
10	204.0
11	70.0
12	135.0
13	126.0
14	185.0
15	341.0
16	596.0
17	729.0
18	1049.0
19	1557.0
20	2246.0
21	3406.0
22	5530.0
23	8535.0
24	12700.0
25	18559.0
26	25889.0
27	34474.0
28	46821.0
29	63006.0
30	82744.0
31	112416.0
32	157463.0
33	222353.0
34	468934.0
35	976554.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.58929328256944	17.029706849207734	11.237654793661873	26.143345074560955
2	16.964438698422093	19.31756836443825	37.0857579001008	26.63223503703885
3	18.86410819543	23.188365164026532	28.55919663380128	29.388330006742187
4	12.6718955551481	15.30784991927028	35.70100453759762	36.319249987984
5	14.796880472869256	36.26278940699522	33.64827389458889	15.292056225546634
6	34.664612746972196	35.41894309185534	16.62731371961682	13.289130441555644
7	30.65457352167445	30.296960422191777	20.454187331575984	18.594278724557793
8	28.461271352063644	32.731773117212086	19.02220124918198	19.78475428154229
9	27.285274899064465	13.952523343846895	18.237675357125685	40.52452639996296
10	15.798456654065198	26.591547665166065	31.50416115630719	26.105834524461542
11	37.50079035649308	20.92200539490663	22.331467053043387	19.245737195556906
12	24.680930270667673	23.48400409855593	28.76577091281039	23.069294717966006
13	29.601883485392033	19.35974143489738	25.02564988617863	26.012725193531956
14	23.749194511948836	19.36293076075925	24.92650965271942	31.961365074572495
15	25.310512471929975	27.101472443395735	21.873356598298187	25.714658486376102
16	26.08166498856202	25.642092119261363	23.397246780243403	24.878996111933215
17	24.374845258727408	25.747032975253862	24.744888417070253	25.13323334894847
18	25.1104403853511	24.973044039425847	25.55407247668382	24.362443098539234
19	25.67037232663844	24.913712248273576	24.950097844743507	24.465817580344474
20	26.048400637598256	24.18602227496447	24.490569415860055	25.27500767157722
21	27.119512888138313	24.132158307792203	24.051994345768545	24.696334458300935
22	26.363259012754543	24.05000093527955	24.599321788717678	24.987418263248234
23	24.848833066096564	24.126091216818104	25.008462497773028	26.016613219312312
24	25.012169762960184	24.736885939147175	24.898778302771234	25.352165995121407
25	25.215105173168627	24.269929546068962	25.00007794069114	25.51488734007127
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	72.0
1	72.0
2	236.0
3	400.0
4	400.0
5	400.0
6	1139.0
7	1878.0
8	1878.0
9	1878.0
10	2285.5
11	2693.0
12	2693.0
13	2693.0
14	3853.5
15	5014.0
16	5014.0
17	5014.0
18	8803.5
19	12593.0
20	12593.0
21	12593.0
22	20780.0
23	28967.0
24	28967.0
25	28967.0
26	43521.5
27	58076.0
28	58076.0
29	58076.0
30	73827.0
31	89578.0
32	89578.0
33	89578.0
34	112369.0
35	135160.0
36	135160.0
37	135160.0
38	157993.5
39	180827.0
40	180827.0
41	180827.0
42	208645.5
43	236464.0
44	236464.0
45	236464.0
46	263346.0
47	290228.0
48	290228.0
49	290228.0
50	299101.5
51	307975.0
52	307975.0
53	307975.0
54	289195.5
55	270416.0
56	270416.0
57	270416.0
58	248703.5
59	226991.0
60	226991.0
61	226991.0
62	199293.5
63	171596.0
64	171596.0
65	171596.0
66	141788.5
67	111981.0
68	111981.0
69	111981.0
70	84210.5
71	56440.0
72	56440.0
73	56440.0
74	43437.0
75	30434.0
76	30434.0
77	30434.0
78	23630.5
79	16827.0
80	16827.0
81	16827.0
82	11471.0
83	6115.0
84	6115.0
85	6115.0
86	4637.5
87	3160.0
88	3160.0
89	3160.0
90	2254.5
91	1349.0
92	1349.0
93	1349.0
94	972.5
95	596.0
96	596.0
97	596.0
98	924.5
99	1253.0
100	1253.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03858335450893447
2	0.005251252401446676
3	0.0016910812818218106
4	0.003515668980629554
5	0.009078436355043405
6	0.01432968875649008
7	0.02318561441655693
8	0.03618023900318769
9	0.048729842199865335
10	0.05843130850084309
11	0.056117197273086936
12	0.06292602453936948
13	0.06492862079415848
14	0.06897831544273175
15	0.0605229090336227
16	0.06648619565899436
17	0.06225849245443982
18	0.07805675179777516
19	0.07516411276307995
20	0.07930281168964386
21	0.07498610420709871
22	0.07854627532672358
23	0.08379752772817026
24	0.07850177318772827
25	0.0794808202456251
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2247083.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.817348359375565
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.35205610225688	33.87331415056445
2	14.97491283597023	14.021714217858058
3	5.368218447309325	7.539772593507206
4	2.442257962254837	4.573601672093735
5	1.326738151387146	3.1057181107582985
6	0.7985621864456794	2.24319384416712
7	0.5033573267752542	1.6496098721816783
8	0.36471856898635735	1.366012503789379
9	0.27338292331536185	1.1519157200723777
>10	1.399760082654145	11.896019131978667
>50	0.10060584035618034	3.271764827993052
>100	0.0782396457352207	7.58632841109772
>500	0.011459951035648858	3.5771878151289385
>1k	0.005729975517824411	4.143847128809362
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3582	0.1594066618812033	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2459	0.10943075978946928	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	2422	0.10778418064664279	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	2307	0.10266643466218203	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	4.450213899531081E-5	0.0	0.0	0.0
6	0.0	4.450213899531081E-5	0.0	0.0	4.450213899531081E-5
7	0.0	4.450213899531081E-5	0.0	4.450213899531081E-5	4.450213899531081E-5
8	0.0	4.450213899531081E-5	0.0	4.450213899531081E-5	4.450213899531081E-5
9	0.0	4.450213899531081E-5	0.0	4.450213899531081E-5	8.900427799062161E-5
10	0.0	4.450213899531081E-5	0.0	4.450213899531081E-5	8.900427799062161E-5
11	0.0	4.450213899531081E-5	0.0	4.450213899531081E-5	8.900427799062161E-5
12	0.0	4.450213899531081E-5	0.0	4.450213899531081E-5	3.5601711196248646E-4
13	0.0	4.450213899531081E-5	0.0	4.450213899531081E-5	4.450213899531081E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGGCGT	255	0.0	13.785752	13
GCGTTAT	150	0.0	13.2954645	1
TAATTGC	385	0.0	13.079287	13
CGTCTTA	110	2.741217E-9	12.95705	15
GGTATCA	1060	0.0	12.901259	1
CGGCGTC	270	0.0	12.668836	14
GTATCAA	2730	0.0	12.592773	1
GTTATTC	200	0.0	12.344688	3
TAATACC	155	1.8189894E-12	12.254431	4
GTATAGA	190	0.0	11.995907	1
AGGCCCG	405	0.0	11.964478	10
ATCGCCA	385	0.0	11.846447	16
CGAGCCG	410	0.0	11.819359	15
AATTGCA	435	0.0	11.795122	14
CGTTATT	170	1.8189894E-12	11.729725	2
CGCATCG	390	0.0	11.693528	13
CGGTCGG	305	0.0	11.526049	10
CGAACGA	240	0.0	11.481387	16
GGTCGGC	310	0.0	11.340146	11
CGACCAT	340	0.0	11.177886	10
>>END_MODULE