FastQCFastQC Report
Thu 2 Feb 2017
SRR4064104_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064104_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2247083
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA24070.10711664856171313No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT22970.10222141327222893No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA22890.10186539616026645No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC22790.10142037477031333No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA29000.026.250721
GGTATCA13250.023.9116571
ATACCGT7100.017.9715026
TCAACGC42850.017.609954
ATCAACG43100.017.5074123
CAACGCA43350.017.4575825
AACGCAG43750.017.398546
TATCAAC43950.017.3686492
ACCGTCG6600.017.3326328
CGTCGTA7150.016.30775610
GTATTAG5700.016.2120341
TACCGTC7400.015.7561197
CCGTCGT7400.015.4598669
ACGCAGA51700.014.7228097
ATCGATT1057.159577E-414.66737829
AAACCGT1057.1632E-414.66646
GTCGTAG7950.014.66639911
GATATAC3350.014.4491051
GTATAAT3050.014.4275721
GTATAGA2753.6379788E-1214.40134051
Produced by FastQC (version 0.11.5)