##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064104_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2247083
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.348753027814283	32.0	32.0	32.0	32.0	32.0
2	31.410331527584873	32.0	32.0	32.0	32.0	32.0
3	31.478743775819584	32.0	32.0	32.0	32.0	32.0
4	31.559986880769426	32.0	32.0	32.0	32.0	32.0
5	31.51589816664538	32.0	32.0	32.0	32.0	32.0
6	35.101939269710996	36.0	36.0	36.0	36.0	36.0
7	35.12003873466178	36.0	36.0	36.0	36.0	36.0
8	35.06570473809823	36.0	36.0	36.0	36.0	36.0
9	35.173493813980166	36.0	36.0	36.0	36.0	36.0
10	35.03031574712639	36.0	36.0	36.0	36.0	36.0
11	35.174748774299836	36.0	36.0	36.0	36.0	36.0
12	35.07124970461705	36.0	36.0	36.0	36.0	36.0
13	35.122423159269154	36.0	36.0	36.0	36.0	36.0
14	35.05564858974947	36.0	36.0	36.0	36.0	36.0
15	35.03680282392773	36.0	36.0	36.0	36.0	36.0
16	35.04846460945145	36.0	36.0	36.0	36.0	36.0
17	35.008276952831736	36.0	36.0	36.0	36.0	36.0
18	35.00907888137643	36.0	36.0	36.0	36.0	36.0
19	35.01083182063146	36.0	36.0	36.0	36.0	36.0
20	34.99989764508031	36.0	36.0	36.0	36.0	36.0
21	34.994363804096245	36.0	36.0	36.0	36.0	36.0
22	34.98263037012874	36.0	36.0	36.0	36.0	36.0
23	34.92676149479125	36.0	36.0	36.0	32.0	36.0
24	34.90015233082178	36.0	36.0	36.0	32.0	36.0
25	34.868103225381525	36.0	36.0	36.0	32.0	36.0
26	34.80923401583297	36.0	36.0	36.0	32.0	36.0
27	34.80909427911653	36.0	36.0	36.0	32.0	36.0
28	34.785180609706	36.0	36.0	36.0	32.0	36.0
29	34.75522710999104	36.0	36.0	36.0	32.0	36.0
30	34.7408907459137	36.0	36.0	36.0	32.0	36.0
31	34.73313268802265	36.0	36.0	36.0	32.0	36.0
32	34.701678131159376	36.0	36.0	36.0	32.0	36.0
33	34.67895311388142	36.0	36.0	36.0	32.0	36.0
34	34.67448331904073	36.0	36.0	36.0	32.0	36.0
35	34.640832581618035	36.0	36.0	36.0	32.0	36.0
36	34.61722597696658	36.0	36.0	36.0	32.0	36.0
37	34.60626020489675	36.0	36.0	36.0	32.0	36.0
38	34.56996826552468	36.0	36.0	36.0	32.0	36.0
39	34.5711769436198	36.0	36.0	36.0	32.0	36.0
40	34.541255040423515	36.0	36.0	36.0	32.0	36.0
41	34.5371363674595	36.0	36.0	36.0	32.0	36.0
42	34.475295750090226	36.0	36.0	36.0	32.0	36.0
43	34.47385165567983	36.0	36.0	36.0	32.0	36.0
44	34.40841081526584	36.0	36.0	36.0	32.0	36.0
45	34.38988501982348	36.0	36.0	36.0	32.0	36.0
46	34.36781774415987	36.0	36.0	36.0	32.0	36.0
47	34.34239856738714	36.0	36.0	36.0	32.0	36.0
48	34.30524462158274	36.0	36.0	36.0	32.0	36.0
49	34.29666505420583	36.0	36.0	36.0	32.0	36.0
50	33.702172994945	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	0.0
17	2.0
18	3.0
19	2.0
20	17.0
21	76.0
22	241.0
23	715.0
24	1798.0
25	4123.0
26	8323.0
27	14965.0
28	24953.0
29	38202.0
30	55582.0
31	79105.0
32	114409.0
33	183844.0
34	385760.0
35	1334962.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.6992154867814	17.89252698530534	11.87375688234883	26.534500645564428
2	16.181480368924046	20.100753201384023	36.54435210217727	27.173414327514656
3	18.066043277651985	23.81312214294869	28.882519118212503	29.238315461186826
4	12.239467790019328	15.97048262124719	35.82680301528693	35.96324657344655
5	14.394172355894286	36.66829396154927	33.49177578220297	15.44575790035348
6	34.69054896229589	35.829362246759786	16.412128472735237	13.067960318209085
7	30.549455048571904	30.393652921278765	20.460099124152624	18.59679290599671
8	28.291478329905928	33.07937446013342	18.90691176071378	19.72223544924687
9	27.317529487576593	13.763435405029176	18.130231307920436	40.7888037994738
10	15.681016225193709	26.821479256682395	31.57653486042435	25.920969657699544
11	37.662015743980746	20.86173128605511	22.266220042802196	19.21003292716195
12	24.550772158331842	23.555746618497444	28.775592124527943	23.11788909864277
13	29.71810859558955	19.284926911521712	25.055505589275995	25.941458903612748
14	23.77043818180119	19.280835893321157	24.920418979111123	32.02830694576653
15	25.369229906532077	27.17281107836584	21.824240066863258	25.633718948238833
16	26.11770192775824	25.67441276758226	23.32984433924059	24.878040965418908
17	24.434878462433296	25.780801154207477	24.7091451450614	25.07517523829783
18	25.180679247163866	24.962662589071307	25.554409374671792	24.302248789093024
19	25.76266721137333	24.843964236269187	24.912230578247957	24.481137974109522
20	26.144384217599885	24.118270095146546	24.461563109129262	25.275782578124307
21	27.144168684467818	24.152645896925033	24.08255502800742	24.620630390599725
22	26.40637022552389	24.01219538787911	24.61453291139297	24.966901475204033
23	24.839178677551413	24.022333191963362	25.07935928696131	26.059128843523915
24	25.09319893639509	24.69120968369992	24.87527118587497	25.34032019403002
25	25.223583820791156	24.18305569302084	25.05349278514948	25.539867701038528
26	24.970749328981373	25.066478797769605	25.18646329235495	24.77630858089407
27	25.601008664707546	24.577256711487802	24.57863637112132	25.24309825268333
28	25.044604174389974	24.36449343053609	25.299078276821362	25.291824118252578
29	24.922909454378285	24.54805959681055	25.264871499439483	25.264159449371682
30	24.866833378059695	24.706708553682983	25.33523631762748	25.09122175062984
31	25.42229180927017	24.504140360121873	24.49888869405307	25.57467913655489
32	25.386892602867995	24.59548394619329	24.246041176570067	25.771582274368647
33	24.81389489818125	24.284295875847747	24.867032816892696	26.034776409078308
34	25.653744497705176	24.357448118995094	25.084322904544116	24.904484478755613
35	26.015435582123136	24.082249525593053	25.165107858600027	24.737207033683784
36	24.90199848324628	24.76946055550215	24.738974161779943	25.58956679947163
37	25.86112211338623	24.564287653370478	24.589743703855348	24.98484652938794
38	24.984823588682758	24.329968116184553	24.950821306435333	25.734386988697356
39	25.809252503692733	24.25992921168448	24.353565962507027	25.57725232211576
40	25.904651706144794	24.47529511242643	24.758959068101156	24.861094113327624
41	24.826713626995602	24.91229428710153	25.26943666741878	24.991555418484086
42	26.103641280814436	24.821020017899112	24.81065081647448	24.264687884811966
43	25.399256803364413	23.853585812509735	25.057831378919026	25.689326005206826
44	24.95846789823311	24.5734309365539	24.673116881325218	25.794984283887768
45	25.143320988280653	24.628246584194237	24.814668450335418	25.413763977189692
46	24.987528153441318	24.54383748371762	24.814502143021485	25.654132219819576
47	25.29345533195076	24.303032460770265	25.228302373810646	25.175209833468326
48	25.835212106269868	24.812263632860027	24.32860383955027	25.02392042131984
49	25.056740504977142	25.046460460545987	24.53472785918742	25.36207117528944
50	25.174194978218118	25.12070264537832	24.49868739071788	25.206414985685683
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	10.0
1	16.5
2	23.0
3	78.5
4	134.0
5	175.0
6	216.0
7	231.5
8	247.0
9	305.0
10	363.0
11	559.5
12	756.0
13	1389.0
14	2022.0
15	2688.0
16	3354.0
17	3577.0
18	3800.0
19	3860.5
20	3921.0
21	4589.0
22	5257.0
23	6271.0
24	7285.0
25	9454.0
26	11623.0
27	14771.5
28	17920.0
29	21594.5
30	25269.0
31	28964.0
32	32659.0
33	37668.5
34	42678.0
35	49891.0
36	57104.0
37	67678.0
38	78252.0
39	85976.5
40	93701.0
41	103557.0
42	113413.0
43	117748.5
44	122084.0
45	134281.5
46	146479.0
47	155569.0
48	164659.0
49	172412.0
50	180165.0
51	176445.0
52	172725.0
53	171081.5
54	169438.0
55	169367.0
56	169296.0
57	163910.0
58	158524.0
59	145617.0
60	132710.0
61	115796.0
62	98882.0
63	85992.0
64	73102.0
65	62554.5
66	52007.0
67	44535.5
68	37064.0
69	32510.0
70	27956.0
71	21544.0
72	15132.0
73	12584.0
74	10036.0
75	7822.0
76	5608.0
77	5035.5
78	4463.0
79	3599.5
80	2736.0
81	2257.5
82	1779.0
83	1462.5
84	1146.0
85	829.0
86	512.0
87	381.0
88	250.0
89	189.5
90	129.0
91	100.0
92	71.0
93	60.5
94	50.0
95	44.5
96	39.0
97	31.0
98	23.0
99	17.0
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009434453467005893
2	3.560171119624865E-4
3	4.895235289484189E-4
4	0.0
5	0.0
6	3.1151497296717567E-4
7	8.900427799062163E-5
8	0.0
9	0.0015575748648358782
10	9.790470578968377E-4
11	8.900427799062163E-5
12	2.2251069497655404E-4
13	5.340256679437298E-4
14	8.900427799062163E-5
15	0.004850733150488878
16	7.565363629202838E-4
17	0.0
18	3.1151497296717567E-4
19	8.900427799062163E-5
20	0.0010235491968921485
21	0.0
22	1.7800855598124326E-4
23	0.0010680513358874595
24	3.560171119624865E-4
25	0.0022696090887608512
26	0.005607269513409162
27	0.006764325127287242
28	0.004272205343549838
29	0.0022251069497655402
30	0.0038271839535967294
31	0.007876878602170014
32	0.003426664702638932
33	0.0044502138995310804
34	0.0029371411736905132
35	0.0029816433126858242
36	0.007965882880160636
37	0.0032486561466576894
38	0.007787874324179392
39	0.0045392181775217024
40	0.002581124061728027
41	0.0035601711196248647
42	0.0019580941157936755
43	0.0014685705868452566
44	0.0011570556138780811
45	0.0012905620308640135
46	9.790470578968377E-4
47	0.002803634756704581
48	0.0021806048107702296
49	4.895235289484189E-4
50	0.001424068447849946
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2247083.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.42996077011403
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.08511942517619	36.12594586065557
2	14.850107382646074	14.680804507123524
3	5.2696962405862555	7.814426353277878
4	2.3410161705441324	4.628653498887961
5	1.2158998303574289	3.005094045747801
6	0.7379695617539048	2.188668389222025
7	0.49480387737984854	1.7120695373450356
8	0.3296934645463704	1.3037388014952054
9	0.23604898032575836	1.050110265359489
>10	1.2730354383346705	11.321741281262836
>50	0.08654040824793918	2.977347157040805
>100	0.06610969159004201	6.767189489554233
>500	0.009517860348813748	3.106384646466287
>1k	0.004441668162779709	3.317826166561381
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	2407	0.10711664856171313	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	2297	0.10222141327222893	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	2289	0.10186539616026645	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	2279	0.10142037477031333	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.450213899531081E-5
2	0.0	0.0	0.0	0.0	4.450213899531081E-5
3	0.0	0.0	0.0	0.0	4.450213899531081E-5
4	0.0	0.0	0.0	0.0	4.450213899531081E-5
5	0.0	8.900427799062161E-5	0.0	0.0	4.450213899531081E-5
6	0.0	8.900427799062161E-5	0.0	0.0	1.7800855598124323E-4
7	0.0	8.900427799062161E-5	0.0	0.0	1.7800855598124323E-4
8	0.0	8.900427799062161E-5	0.0	0.0	1.7800855598124323E-4
9	0.0	8.900427799062161E-5	0.0	0.0	1.7800855598124323E-4
10	0.0	8.900427799062161E-5	0.0	0.0	1.7800855598124323E-4
11	0.0	8.900427799062161E-5	0.0	0.0	1.7800855598124323E-4
12	0.0	8.900427799062161E-5	0.0	0.0	2.2251069497655404E-4
13	0.0	8.900427799062161E-5	0.0	0.0	2.2251069497655404E-4
14	0.0	8.900427799062161E-5	0.0	0.0	2.2251069497655404E-4
15	0.0	8.900427799062161E-5	0.0	4.450213899531081E-5	3.5601711196248646E-4
16	0.0	8.900427799062161E-5	0.0	4.450213899531081E-5	4.005192509577973E-4
17	0.0	8.900427799062161E-5	0.0	4.450213899531081E-5	4.005192509577973E-4
18	0.0	8.900427799062161E-5	0.0	4.450213899531081E-5	4.450213899531081E-4
19	0.0	8.900427799062161E-5	0.0	8.900427799062161E-5	4.450213899531081E-4
20	0.0	8.900427799062161E-5	0.0	1.3350641698593244E-4	4.895235289484189E-4
21	0.0	8.900427799062161E-5	0.0	1.7800855598124323E-4	5.340256679437298E-4
22	0.0	8.900427799062161E-5	0.0	1.7800855598124323E-4	5.340256679437298E-4
23	0.0	8.900427799062161E-5	0.0	2.2251069497655404E-4	5.785278069390406E-4
24	0.0	8.900427799062161E-5	0.0	3.5601711196248646E-4	5.785278069390406E-4
25	0.0	8.900427799062161E-5	0.0	5.340256679437298E-4	6.230299459343513E-4
26	0.0	8.900427799062161E-5	0.0	8.010385019155946E-4	7.120342239249729E-4
27	0.0	8.900427799062161E-5	0.0	0.0012460598918687027	7.120342239249729E-4
28	0.0	8.900427799062161E-5	0.0	0.0034266647026389326	7.120342239249729E-4
29	0.0	8.900427799062161E-5	0.0	0.006853329405277865	7.120342239249729E-4
30	0.0	8.900427799062161E-5	0.0	0.013261637420602622	7.120342239249729E-4
31	0.0	8.900427799062161E-5	0.0	0.032175046493609714	7.565363629202838E-4
32	0.0	8.900427799062161E-5	0.0	0.05108845556661681	8.010385019155946E-4
33	0.0	8.900427799062161E-5	0.0	0.06955684324967079	8.010385019155946E-4
34	0.0	8.900427799062161E-5	0.0	0.09243094269326055	8.455406409109054E-4
35	0.0	8.900427799062161E-5	0.0	0.11779716192058771	8.455406409109054E-4
36	0.0	8.900427799062161E-5	0.0	0.15482294156468632	8.455406409109054E-4
37	0.0	1.7800855598124323E-4	0.0	0.2141887949844309	8.455406409109054E-4
38	0.0	1.7800855598124323E-4	0.0	0.2960282285968075	8.455406409109054E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2900	0.0	26.25072	1
GGTATCA	1325	0.0	23.911657	1
ATACCGT	710	0.0	17.971502	6
TCAACGC	4285	0.0	17.60995	4
ATCAACG	4310	0.0	17.507412	3
CAACGCA	4335	0.0	17.457582	5
AACGCAG	4375	0.0	17.39854	6
TATCAAC	4395	0.0	17.368649	2
ACCGTCG	660	0.0	17.332632	8
CGTCGTA	715	0.0	16.307756	10
GTATTAG	570	0.0	16.212034	1
TACCGTC	740	0.0	15.756119	7
CCGTCGT	740	0.0	15.459866	9
ACGCAGA	5170	0.0	14.722809	7
ATCGATT	105	7.159577E-4	14.667378	29
AAACCGT	105	7.1632E-4	14.6664	6
GTCGTAG	795	0.0	14.666399	11
GATATAC	335	0.0	14.449105	1
GTATAAT	305	0.0	14.427572	1
GTATAGA	275	3.6379788E-12	14.4013405	1
>>END_MODULE