FastQCFastQC Report
Thu 2 Feb 2017
SRR4064103_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064103_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1809897
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT75460.4169298031876952No Hit
TATCAACGCAGAGTACTTTTTTTTT53120.2934973647671663No Hit
GTACTTTTTTTTTTTTTTTTTTTTT40200.22211208704141727No Hit
GTACATGGGGTGGTATCAACGCAAA30320.1675233452511386No Hit
GGTATCAACGCAGAGTACTTTTTTT27090.14967702581970133No Hit
GAGTACTTTTTTTTTTTTTTTTTTT26650.1472459482500938No Hit
GTCCTAAAGTGTGTATTTCTCATTT26590.14691443767242005No Hit
GTCCTACAGTGGACATTTCTAAATT24570.13575358155740352No Hit
ACGCAGAGTACTTTTTTTTTTTTTT24250.1339855251431435No Hit
GTCCTACAGTGTGCATTTCTCATTT23370.1291233700039284No Hit
CTTTAGGACGTGAAATATGGCGAGG22390.12370869723525703No Hit
GATATACACTGTTCTACAAATCCCG22380.1236534454723114No Hit
CTGTAGGACGTGGAATATGGCAAGA21840.12066985027324759No Hit
TTGTAGAACAGTGTATATCAATGAG20830.11508942221573935No Hit
GTATCAACGCAGAGTACATGGGGTG19100.10553086722614603No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTGCG456.765102E-414.7771967
GTCCTAG1950.013.1482391
CCAACGA2150.012.817348519
TCCAACG3350.012.48126318
CCAACGT1155.304173E-912.394587519
ATACGCT2050.012.0493068
CTATACT1750.011.9407374
GATATAC8750.011.9377671
ATAAGAC1201.0062649E-811.8712513
CGTTATT1052.7406168E-711.7552662
TATTTCG658.034633E-411.6905555
CGCCCCT1552.5465852E-1111.6434155
CGCATCG2450.011.63348313
GGCGAGG7100.011.51009119
TCTTATA1657.2759576E-1211.5086532
TGCGGTA2150.011.4907815
CTAACAT752.0802645E-411.3964013
TCTAGAT2600.011.3205312
ACTGTTC9800.011.2453898
CGACCCG2200.011.2255895
Produced by FastQC (version 0.11.5)