FastQCFastQC Report
Thu 2 Feb 2017
SRR4064103_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064103_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1809897
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA18990.10492309783374414No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18500.10221576144940844No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA450.001398998424.4428967
GTCCTAC19200.020.5113811
GTAGGAC20200.019.3854453
CGTCTTA1605.2750693E-1119.2487815
GATATAC11550.019.2391
TCCTACA21100.019.0798852
TAGGACC14000.019.013084
ATTTCGT9100.018.85646842
ACGATTA708.127412E-418.85646640
TGTAGGA21100.018.662842
GCGAGAA13000.018.6147220
TTAGGAC12100.018.544793
TAGAACG951.5986985E-518.525144
TAGGACA7900.018.378584
ATCACGG10850.018.25071735
TGGCGAG22300.018.2494618
GATTTCG9300.018.21440141
ACTGTTC12950.018.1759518
GTATCAA59200.018.135991
TATGGCG22650.018.0650816
Produced by FastQC (version 0.11.5)