FastQCFastQC Report
Thu 2 Feb 2017
SRR4064102_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064102_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1456686
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT42430.29127759860395447No Hit
TATCAACGCAGAGTACTTTTTTTTT30850.21178208618741445No Hit
GTACTTTTTTTTTTTTTTTTTTTTT22810.15658831072722604No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG17670.12130273785839914No Hit
GATTAAGAGGGACGGCCGGGGGCAT16850.11567352195325555No Hit
GAATAGGACCGCGGTTCTATTTTGT16010.109907008099206No Hit
GGTATCAACGCAGAGTACTTTTTTT15680.10764159194225797No Hit
GAGTACTTTTTTTTTTTTTTTTTTT14800.10160048219039655No Hit
CTATTGGAGCTGGAATTACCGCGGC14790.10153183321594358No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGTA400.005278637614.25010313
CGCATCG2150.014.13963613
GCGTTAT1152.5465852E-1114.0450271
TTGCGCG1500.013.93678518
ATTTGCG1600.013.65822516
GTGTTAT852.6949965E-713.4132431
CCGCGCA500.001498221813.3014679
CGAACGA1301.4551915E-1113.15574916
TCCTATA655.4727763E-513.1485222
CGACCCG1450.013.0999445
GTCCTAC3700.012.8392541
AACCGCG1800.012.6671947
ACATCGT751.4809339E-512.6645826
GCATCGC2450.012.40867914
CGCCACT1155.307811E-912.39352317
ATCGCCA2550.012.29589616
CGTTATT1251.4188117E-912.1550782
GCCTTAG550.003065752612.0922421
GTACGAG550.003065752612.0922421
TCCCGAC1501.4551915E-1112.0284612
Produced by FastQC (version 0.11.5)