FastQCFastQC Report
Thu 2 Feb 2017
SRR4064102_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064102_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1456686
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA17290.11869407682918624No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA16450.1129275629751367No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT15230.1045523880918743No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA14630.10043344962469605No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGG353.2189643E-431.4272742
TAGATCG502.3626544E-630.7987255
GTATCGA454.07126E-529.3321199
TCGTACG450.001398688224.44343441
CCCTATA654.933064E-420.307552
TATCGAG654.934206E-420.30685410
TATGCGA808.9930385E-519.24920517
TTAGATC1404.08545E-918.8563634
TACCGTA851.4315412E-418.11689812
GTATCAA32800.018.0437951
GTGTATA2200.018.0011081
TAGGACG3600.017.7214894
GGCGTTA3700.017.2425342
CGCAATA4450.016.80944836
ACCGTCG4600.016.7384388
TAATACT2650.016.6030884
GACAGTA1206.4108663E-616.4993177
TAATCCC3250.016.2454815
AGTATCG953.3479568E-416.2098568
TTAGGAC3400.016.17583
Produced by FastQC (version 0.11.5)