##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064102_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1456686
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.28891881984175	32.0	32.0	32.0	32.0	32.0
2	31.418827393137573	32.0	32.0	32.0	32.0	32.0
3	31.490915681210637	32.0	32.0	32.0	32.0	32.0
4	31.574114805798917	32.0	32.0	32.0	32.0	32.0
5	31.52061116808976	32.0	32.0	32.0	32.0	32.0
6	35.110690979387456	36.0	36.0	36.0	36.0	36.0
7	35.13235865519405	36.0	36.0	36.0	36.0	36.0
8	35.07619624270433	36.0	36.0	36.0	36.0	36.0
9	35.18090240449898	36.0	36.0	36.0	36.0	36.0
10	35.03347049398429	36.0	36.0	36.0	36.0	36.0
11	35.185013105089226	36.0	36.0	36.0	36.0	36.0
12	35.08431329744365	36.0	36.0	36.0	36.0	36.0
13	35.12838937149118	36.0	36.0	36.0	36.0	36.0
14	35.070188084460206	36.0	36.0	36.0	36.0	36.0
15	35.049287904188	36.0	36.0	36.0	36.0	36.0
16	35.052137523117544	36.0	36.0	36.0	36.0	36.0
17	35.01781784131927	36.0	36.0	36.0	36.0	36.0
18	35.013630940367385	36.0	36.0	36.0	36.0	36.0
19	35.017982598857955	36.0	36.0	36.0	36.0	36.0
20	35.007441548830705	36.0	36.0	36.0	36.0	36.0
21	35.00039198564413	36.0	36.0	36.0	36.0	36.0
22	34.988946142133585	36.0	36.0	36.0	36.0	36.0
23	34.936503817569466	36.0	36.0	36.0	36.0	36.0
24	34.90165485217816	36.0	36.0	36.0	32.0	36.0
25	34.877587208224696	36.0	36.0	36.0	32.0	36.0
26	34.819372877888576	36.0	36.0	36.0	32.0	36.0
27	34.81367501300898	36.0	36.0	36.0	32.0	36.0
28	34.79290595227798	36.0	36.0	36.0	32.0	36.0
29	34.76006290992019	36.0	36.0	36.0	32.0	36.0
30	34.746721668225	36.0	36.0	36.0	32.0	36.0
31	34.73520305680154	36.0	36.0	36.0	32.0	36.0
32	34.68935103378491	36.0	36.0	36.0	32.0	36.0
33	34.671578500788776	36.0	36.0	36.0	32.0	36.0
34	34.654836388899184	36.0	36.0	36.0	32.0	36.0
35	34.6142497422231	36.0	36.0	36.0	32.0	36.0
36	34.58890454085506	36.0	36.0	36.0	32.0	36.0
37	34.576900581182215	36.0	36.0	36.0	32.0	36.0
38	34.53011218615405	36.0	36.0	36.0	32.0	36.0
39	34.5253060714526	36.0	36.0	36.0	32.0	36.0
40	34.506097401910914	36.0	36.0	36.0	32.0	36.0
41	34.48216362345763	36.0	36.0	36.0	32.0	36.0
42	34.41013505999234	36.0	36.0	36.0	32.0	36.0
43	34.40060795531775	36.0	36.0	36.0	32.0	36.0
44	34.33399442295732	36.0	36.0	36.0	32.0	36.0
45	34.29377916723302	36.0	36.0	36.0	32.0	36.0
46	34.25545244479593	36.0	36.0	36.0	32.0	36.0
47	34.23029328214866	36.0	36.0	36.0	32.0	36.0
48	34.17801777459246	36.0	36.0	36.0	32.0	36.0
49	34.1548844431813	36.0	36.0	36.0	32.0	36.0
50	33.56860229315034	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	2.0
19	2.0
20	14.0
21	59.0
22	161.0
23	503.0
24	1272.0
25	2958.0
26	5763.0
27	10359.0
28	17120.0
29	25704.0
30	36673.0
31	51504.0
32	74329.0
33	119387.0
34	250079.0
35	860795.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.84749735338581	18.02903792313216	11.76957224398556	26.353892479496466
2	15.933810950410388	20.603003001353766	37.274589544964016	26.18859650327183
3	17.769928879369527	24.86414311997144	29.098429304994923	28.267498695664113
4	11.990573122828119	16.053974569673905	36.61647053654665	35.33898177095132
5	13.896337302617038	37.416986227642745	33.44028843553106	15.246388034209158
6	33.455185513938176	36.41080454704284	16.99930595743892	13.13470398158007
7	29.999395889024814	30.79483155601139	21.034938209058097	18.170834345905707
8	28.075096486133592	32.81345464980099	19.50269309926779	19.608755764797632
9	27.81572203431253	13.502312467691699	18.4337690992257	40.24819639877007
10	15.612278382122561	26.48272708924577	31.82784892158438	26.077145607047285
11	37.126875661604494	20.896473227586455	23.034133643077507	18.942517467731548
12	24.93407977702798	23.716177485180392	28.98457799730209	22.365164740489536
13	29.54017946325781	19.46162395644333	25.464190070962882	25.534006509335978
14	23.639084633946254	19.701308107106204	25.47819192206963	31.18141533687791
15	25.17751749430358	27.52974872598631	22.30936478154518	24.983368998164927
16	25.627575639623608	25.58782763783598	24.020081322077576	24.764515400462834
17	23.846388308805054	25.88842070288312	25.330510487503826	24.934680500808
18	24.734019548481964	24.73175413232502	26.56392661150035	23.970299707692668
19	25.61567832738147	24.554845725159712	25.727232910867542	24.102243036591275
20	25.986749293082816	23.96989861170321	25.200439081553426	24.842913013660546
21	27.140509347930852	23.789546957957995	24.537546183597563	24.53239751051359
22	26.074495223397797	24.113259979515117	25.06377498482856	24.748469812258527
23	24.28570055578744	24.079339755277786	25.767502910744494	25.867456778190284
24	24.78219291964805	24.62519196061727	25.638112446187527	24.954502673547157
25	24.817887557451833	24.27376420634948	25.76409650878211	25.144251727416584
26	24.527690494219833	25.163409237418982	26.050806696878183	24.258093571483002
27	25.27914167710187	24.67836233221292	25.10442068147367	24.938075309211538
28	24.365986490763287	24.584778732872316	25.930484892213755	25.118749884150642
29	24.437493950163766	24.636100205196716	25.725860209480512	25.200545635159006
30	24.282714224614644	24.896731306427064	26.103003013104843	24.71755145585345
31	25.21952016564762	24.607747156707536	24.936186397507594	25.23654628013725
32	24.765351039787348	24.877732651217457	24.86036395989686	25.49655234909834
33	24.30433364798764	24.532120483995538	25.56766497897537	25.59588088904145
34	25.304996303179127	24.522176462955933	25.698500058696045	24.4743271751689
35	25.865393790817144	24.395993818689334	25.435708945669134	24.302903444824388
36	24.51576538693114	25.006916901749786	25.27370677966218	25.20361093165689
37	25.508291662204353	24.850409571590586	24.997665867569562	24.64363289863549
38	24.61580921728337	24.754834652415816	25.472137243163377	25.157218887137432
39	25.34948844214965	24.59507534176513	24.95213262951651	25.103303586568714
40	25.532522524254247	24.764665822722243	25.41135425619846	24.29145739682505
41	24.511446157817343	25.166718612483745	25.734941777205993	24.58689345249292
42	25.794180926476905	25.227472749265782	25.16918864963499	23.80915767462232
43	24.842912150941636	24.49520103743541	25.49227037851427	25.169616433108686
44	24.533492051069768	25.12943870763122	25.04348948357697	25.293579757722046
45	24.78823231390565	25.3414816152216	24.888529773791817	24.98175629708094
46	24.490155978941026	24.95978844914191	25.140749009213476	25.409306562703588
47	24.849725125354922	24.82137267070512	25.502998632491774	24.825903571448187
48	25.452218822490767	25.35645120210675	24.522346121342725	24.66898385405976
49	24.758063021347844	25.368354499028957	24.782708087769386	25.090874391853813
50	24.781229426435626	25.79402373781719	24.495027333020747	24.92971950272644
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	113.0
1	78.0
2	43.0
3	152.0
4	261.0
5	274.0
6	287.0
7	284.5
8	282.0
9	415.5
10	549.0
11	774.5
12	1000.0
13	1822.0
14	2644.0
15	3466.5
16	4289.0
17	4639.5
18	4990.0
19	5068.0
20	5146.0
21	5485.5
22	5825.0
23	6552.0
24	7279.0
25	8019.5
26	8760.0
27	10763.0
28	12766.0
29	14991.5
30	17217.0
31	20457.5
32	23698.0
33	28071.5
34	32445.0
35	37396.0
36	42347.0
37	48897.0
38	55447.0
39	60587.5
40	65728.0
41	71082.0
42	76436.0
43	78265.5
44	80095.0
45	86934.5
46	93774.0
47	99906.0
48	106038.0
49	109163.5
50	112289.0
51	108416.0
52	104543.0
53	102829.0
54	101115.0
55	101879.0
56	102643.0
57	99494.5
58	96346.0
59	87635.5
60	78925.0
61	69390.0
62	59855.0
63	52977.5
64	46100.0
65	39333.0
66	32566.0
67	28639.5
68	24713.0
69	22246.0
70	19779.0
71	15334.0
72	10889.0
73	9233.0
74	7577.0
75	5855.0
76	4133.0
77	3632.5
78	3132.0
79	2575.0
80	2018.0
81	1581.0
82	1144.0
83	962.5
84	781.0
85	538.5
86	296.0
87	219.5
88	143.0
89	110.0
90	77.0
91	62.0
92	47.0
93	44.0
94	41.0
95	41.0
96	41.0
97	30.5
98	20.0
99	17.0
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007414089240920831
2	6.864897445297064E-4
3	4.118938467178239E-4
4	0.0
5	0.0
6	2.0594692335891194E-4
7	0.0
8	0.0
9	0.001167032565700501
10	8.237876934356477E-4
11	0.0
12	6.864897445297064E-5
13	6.178407700767358E-4
14	6.864897445297064E-5
15	0.004736779237254974
16	6.178407700767358E-4
17	0.0
18	0.0
19	0.0
20	6.178407700767358E-4
21	0.0
22	1.3729794890594127E-4
23	9.610856423415891E-4
24	4.8054282117079454E-4
25	0.00212811820804209
26	0.004599481288349033
27	0.005354620007331711
28	0.0036383956460074446
29	0.0024027141058539725
30	0.0033637997481955617
31	0.006864897445297065
32	0.002883256927024767
33	0.0041875874416312095
34	0.001990820259136149
35	0.002540012054759914
36	0.007482738215373801
37	0.002883256927024767
38	0.008100578985450538
39	0.0039129915438193275
40	0.002334065131401002
41	0.0027459589781188257
42	0.0019221712846831782
43	0.001167032565700501
44	8.237876934356477E-4
45	0.0013043305146064423
46	0.0010297346167945597
47	0.0015102774379653543
48	0.0024027141058539725
49	3.432448722648532E-4
50	0.001578926412418325
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1456686.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.518334954535796
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.77528634944507	39.03816583426575
2	13.894384854688266	14.316311458621184
3	4.540721909572939	7.017912968183341
4	1.9292008082422578	3.9755685373434333
5	1.0048732907991702	2.5884699391129153
6	0.601294285454175	1.8586608242565867
7	0.404612945650577	1.459148968267747
8	0.2770709886132153	1.1419388798047998
9	0.2055770075187373	0.9531886619071289
>10	1.191509614661692	11.193906823714814
>50	0.0883076135602036	3.139824391643061
>100	0.07495794346649792	8.12117203801474
>500	0.009386452559380684	3.3343146182475305
>1k	0.0028159357678142053	1.8614160566170739
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1729	0.11869407682918624	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1645	0.1129275629751367	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1523	0.1045523880918743	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA	1463	0.10043344962469605	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.864897445297065E-5
2	0.0	0.0	0.0	0.0	6.864897445297065E-5
3	0.0	0.0	0.0	1.372979489059413E-4	6.864897445297065E-5
4	0.0	0.0	0.0	1.372979489059413E-4	6.864897445297065E-5
5	0.0	0.0	0.0	1.372979489059413E-4	6.864897445297065E-5
6	0.0	0.0	0.0	1.372979489059413E-4	6.864897445297065E-5
7	0.0	0.0	0.0	1.372979489059413E-4	6.864897445297065E-5
8	0.0	0.0	0.0	1.372979489059413E-4	6.864897445297065E-5
9	0.0	0.0	0.0	1.372979489059413E-4	6.864897445297065E-5
10	0.0	0.0	0.0	2.0594692335891194E-4	6.864897445297065E-5
11	0.0	0.0	0.0	2.0594692335891194E-4	6.864897445297065E-5
12	0.0	0.0	0.0	2.745958978118826E-4	2.0594692335891194E-4
13	0.0	0.0	0.0	2.745958978118826E-4	2.0594692335891194E-4
14	0.0	0.0	0.0	3.432448722648532E-4	2.0594692335891194E-4
15	0.0	0.0	0.0	3.432448722648532E-4	2.745958978118826E-4
16	0.0	0.0	0.0	4.118938467178239E-4	2.745958978118826E-4
17	0.0	0.0	0.0	4.118938467178239E-4	2.745958978118826E-4
18	0.0	0.0	0.0	4.118938467178239E-4	3.432448722648532E-4
19	0.0	0.0	0.0	4.118938467178239E-4	3.432448722648532E-4
20	0.0	0.0	0.0	4.118938467178239E-4	6.178407700767358E-4
21	0.0	0.0	0.0	5.491917956237652E-4	6.178407700767358E-4
22	0.0	0.0	0.0	8.237876934356477E-4	6.178407700767358E-4
23	0.0	0.0	0.0	0.0015789264124183248	6.178407700767358E-4
24	0.0	0.0	0.0	0.0024713630803069432	6.178407700767358E-4
25	0.0	0.0	0.0	0.0032265017992896203	6.178407700767358E-4
26	0.0	0.0	0.0	0.003981640518272297	6.178407700767358E-4
27	0.0	0.0	0.0	0.005217322058425769	6.178407700767358E-4
28	0.0	0.0	0.0	0.008100578985450536	6.178407700767358E-4
29	0.0	0.0	0.0	0.013935741813953042	6.178407700767358E-4
30	0.0	0.0	0.0	0.024027141058539727	6.178407700767358E-4
31	0.0	0.0	0.0	0.05313430622659928	6.178407700767358E-4
32	0.0	0.0	0.0	0.08272201421582963	6.178407700767358E-4
33	0.0	0.0	0.0	0.11031890194592384	6.864897445297064E-4
34	0.0	0.0	0.0	0.1407990466030428	7.551387189826771E-4
35	0.0	0.0	0.0	0.17649651331858754	7.551387189826771E-4
36	0.0	0.0	0.0	0.23182758672768186	7.551387189826771E-4
37	0.0	0.0	0.0	0.3059684791368902	7.551387189826771E-4
38	0.0	0.0	0.0	0.4040678636301852	7.551387189826771E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTACGG	35	3.2189643E-4	31.42727	42
TAGATCG	50	2.3626544E-6	30.798725	5
GTATCGA	45	4.07126E-5	29.332119	9
TCGTACG	45	0.0013986882	24.443434	41
CCCTATA	65	4.933064E-4	20.30755	2
TATCGAG	65	4.934206E-4	20.306854	10
TATGCGA	80	8.9930385E-5	19.249205	17
TTAGATC	140	4.08545E-9	18.856363	4
TACCGTA	85	1.4315412E-4	18.116898	12
GTATCAA	3280	0.0	18.043795	1
GTGTATA	220	0.0	18.001108	1
TAGGACG	360	0.0	17.721489	4
GGCGTTA	370	0.0	17.24253	42
CGCAATA	445	0.0	16.809448	36
ACCGTCG	460	0.0	16.738438	8
TAATACT	265	0.0	16.603088	4
GACAGTA	120	6.4108663E-6	16.499317	7
TAATCCC	325	0.0	16.245481	5
AGTATCG	95	3.3479568E-4	16.209856	8
TTAGGAC	340	0.0	16.1758	3
>>END_MODULE