FastQCFastQC Report
Thu 2 Feb 2017
SRR4064101_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064101_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences269221
Sequences flagged as poor quality0
Sequence length25
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT52501.9500707597104237No Hit
TATCAACGCAGAGTACTTTTTTTTT36801.36690674204464No Hit
GTACTTTTTTTTTTTTTTTTTTTTT35591.321962254058933No Hit
GTACATGGGGTGGTATCAACGCAAA27171.009208048406328No Hit
GAGTACTTTTTTTTTTTTTTTTTTT21950.8153152985836915No Hit
ACGCAGAGTACTTTTTTTTTTTTTT20000.7428840989373042No Hit
GGTATCAACGCAGAGTACTTTTTTT19750.733598047700588No Hit
GTCCTAAAGTGTGTATTTCTCATTT19650.7298836272059015No Hit
GTCCTACAGTGGACATTTCTAAATT18840.6997968211989406No Hit
CTGTAGGACGTGGAATATGGCAAGA17970.6674813628951679No Hit
CTTTAGGACGTGAAATATGGCGAGG16710.6206796646621178No Hit
GTATCAACGCAGAGTACATGGGGTG16330.6065648667823089No Hit
GTCCTACAGTGTGCATTTCTCATTT13560.5036754190794923No Hit
GTACATGGGTGGTATCAACGCAAAA12740.4732171710230628No Hit
TATCAACGCAGAGTACATGGGGTGG11020.40932913851445474No Hit
CTGTAGGACCTGGAATATGGCGAGA10110.37552791201280733No Hit
GTACATGGGAGTGGTATCAACGCAA9930.36884195512237156No Hit
GCAGAGTACTTTTTTTTTTTTTTTT9750.36215599823193584No Hit
GGTATCAACGCAGAGTACATGGGGT8560.3179543943451662No Hit
ATTTAGAAATGTCCACTGTAGGACG7850.2915820088328919No Hit
CTGAAGGACCTGGAATATGGCGAGA7680.28526749399192486No Hit
GATATACACTGTTCTACAAATCCCG7290.2707812540626474No Hit
GTACATGGAAGCAGTGGTATCAACG7230.2685526017658355No Hit
GTATCAACGCAGAGTACATGGGAGT6510.24180877420409255No Hit
GTCCTTCAGTGTGCATTTCTCATTT6470.24032300600621792No Hit
ACGCAGAGTACATGGGGTGGTATCA6440.23920867985781197No Hit
GTATCAACGCAGAGTACATGGGAAG6300.23400849116525083No Hit
TTTCTAAATTTTCCACCTTTTTCAG6240.23177983886843895No Hit
ATCAACGCAGAGTACTTTTTTTTTT6160.22880830247268974No Hit
TCCATGTACTCTGCGTTGATACCAC6070.22546532402747185No Hit
GAATATGGCAAGAAAACTGAAAATC6010.22323667173065995No Hit
TTGTAGAACAGTGTATATCAATGAG5660.2102361999992571No Hit
CTGTAGGACATGGAATATGGCAAGA5350.1987214964657289No Hit
CCCATGTACTCTGCGTTGATACCAC5180.1924069816247618No Hit
GTACATGGGAAGCAGTGGTATCAAC4920.18274948833857688No Hit
GGAATATGGCGAGAAAACTGAAAAT4840.17977795194282764No Hit
GTGTATATCAATGAGTTACAATGAG4770.17717785759654708No Hit
GTATCAACGCAGAGTACATGGGTGG4500.16714892226089348No Hit
GTGTATATCAATGAGTTACAATGAA4380.16269161766726964No Hit
GGTATCAACGCAGAGTACATGGGAA4300.15972008127152043No Hit
TATCAACGCAGAGTACATGGGAGTG4030.14969114593586683No Hit
GATATACACTGTTCTACAATGCCGG4000.14857681978746085No Hit
GTTCTACAGTGTGGTTTTTATCATT3920.14560528339171164No Hit
GCCATATTCCACGTCCTACAGTGGA3850.14300518904543108No Hit
GAGTACATGGGGTGGTATCAACGCA3840.14263374699596243No Hit
GAAATATGGCGAGGAAAACTGAAAA3810.14151942084755648No Hit
GTCCACTGTAGGACGTGGAATATGG3750.13929076855074457No Hit
CATGTACTCTGCGTTGATACCACTG3430.12740462296774768No Hit
CCATATTCCAGGTCCTTCAGTGTGC3420.12703318091827903No Hit
GGTATCAACGCAGAGTACATGGGAG3420.12703318091827903No Hit
CCATATTTCACGTCCTAAAGTGTGT3410.12666173886881038No Hit
ACAGTGGACATTTCTAAATTTTCCA3370.12517597067093578No Hit
TATCAACGCAGAGTACATGGGAAGC3350.12443308657199847No Hit
ACCTGGAATATGGCGAGAAAACTGA3320.12331876042359251No Hit
GTTCTACAAATCCCGTTTCCAACGA3280.1218329922257179No Hit
GTATCAACGCAGAGTACATGGGCAG3240.12034722402784329No Hit
TATCAACGCAGAGTACATGGGTGGT3180.11811857173103138No Hit
ATTCCAGGTCCTTCAGTGTGCATTT3040.11291838303847027No Hit
GACCTGGAATATGGCGAGAAAACTG3030.1125469409890016No Hit
ATACACACTTTAGGACGTGAAATAT3010.1118040568900643No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT3000.11143261484059565No Hit
TTCCAGGTCCTTCAGTGTGCATTTC2980.11068973074165835No Hit
CTGTAGAACATATTAGATGAGTGAG2940.10920396254378373No Hit
GTACATGGGGTATCAACGCAAAAAA2930.10883252049431508No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT2920.10846107844484643No Hit
CAACGCAGAGTACTTTTTTTTTTTT2880.10697531024697182No Hit
CCTAAAGTGTGTATTTCTCATTTTC2840.1054895420490972No Hit
CACTTTAGGACGTGAAATATGGCGA2800.10400377385122259No Hit
GGTGGTATCAACGCAAAAAAAAAAA2790.10363233180175394No Hit
GGTATCAACGCAGAGTACATGGGTG2760.10251800565334798No Hit
GAAATACACACTTTAGGACGTGAAA2720.10103223745547339No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG307.6879107E-419.0044611
TTTAAAC307.716808E-418.9938683
AAAGGTC402.7455593E-416.62890419
GAGGCAC350.002165914316.2834857
CCTAGAG350.002168605816.2804583
CCAACGT601.4522739E-615.83705119
TCCAACG1300.015.34975818
TAGGACC3350.015.0250014
TATGGTG400.005255905414.25334616
GTTCATG400.005255905414.2533461
GGCACTG400.00526889114.2480499
ACACCAT400.00526889114.2480496
AGGCACT400.00526889114.2480498
CACCATT400.00526889114.2480497
TCTACAC602.5605113E-514.2454013
CTACACC602.5605113E-514.2454014
GCCGGTT759.582145E-713.93401511
CCGGTTT759.582145E-713.93401512
TGCCGGT759.582145E-713.93401510
ATAGGAC551.9560393E-413.8137223
Produced by FastQC (version 0.11.5)