##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4064101_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 269221 Sequences flagged as poor quality 0 Sequence length 25 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.95680500406729 32.0 32.0 32.0 32.0 32.0 2 30.82444534415963 32.0 32.0 32.0 32.0 32.0 3 30.900527819152295 32.0 32.0 32.0 32.0 32.0 4 30.817855962202056 32.0 32.0 32.0 32.0 32.0 5 30.892341236382006 32.0 32.0 32.0 32.0 32.0 6 34.44661077701962 36.0 36.0 36.0 32.0 36.0 7 34.240928456546854 36.0 36.0 36.0 32.0 36.0 8 34.16447453950472 36.0 36.0 36.0 32.0 36.0 9 34.130431875670915 36.0 36.0 36.0 32.0 36.0 10 34.041464075982184 36.0 36.0 36.0 32.0 36.0 11 34.36276144877257 36.0 36.0 36.0 32.0 36.0 12 34.21046649406993 36.0 36.0 36.0 32.0 36.0 13 34.25193428447261 36.0 36.0 36.0 32.0 36.0 14 34.189784600755516 36.0 36.0 36.0 32.0 36.0 15 34.22444014397094 36.0 36.0 36.0 32.0 36.0 16 34.1763755427697 36.0 36.0 36.0 32.0 36.0 17 34.11945947752962 36.0 36.0 36.0 32.0 36.0 18 34.15261810928568 36.0 36.0 36.0 32.0 36.0 19 34.02419573510239 36.0 36.0 36.0 32.0 36.0 20 33.90420509544204 36.0 36.0 36.0 32.0 36.0 21 33.847634471307956 36.0 36.0 36.0 32.0 36.0 22 33.8521512066295 36.0 36.0 36.0 32.0 36.0 23 33.94995561267509 36.0 36.0 36.0 32.0 36.0 24 33.93161751869282 36.0 36.0 36.0 32.0 36.0 25 33.65818045397647 36.0 36.0 36.0 27.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 2.0 4 0.0 5 1.0 6 19.0 7 7.0 8 12.0 9 25.0 10 21.0 11 12.0 12 16.0 13 14.0 14 73.0 15 108.0 16 133.0 17 200.0 18 215.0 19 268.0 20 416.0 21 640.0 22 937.0 23 1315.0 24 1835.0 25 2448.0 26 3248.0 27 4161.0 28 5696.0 29 7137.0 30 9324.0 31 12623.0 32 17749.0 33 25016.0 34 53889.0 35 121661.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.83479863552249 18.741128295071977 12.844742376836582 21.57933069256895 2 11.480056165167195 21.33013380088111 48.5197209571852 18.67008907676649 3 19.4608087126418 30.96821163518041 29.029633976925762 20.541345675252025 4 9.707663162586828 17.16243824523606 38.43059321719104 34.699305374986075 5 9.285937174953194 43.1528425807602 34.21201224404886 13.349208000237747 6 26.894327790409108 39.53457268958138 20.2658523174773 13.305247202532211 7 25.328993810235033 33.7992376111817 22.059490106035952 18.812278472547312 8 25.54474650336658 39.14594449976961 19.325123738462224 15.984185258401581 9 29.47911788971392 13.155840970410507 21.057893132948323 36.30714800692726 10 17.35513845010221 28.84668277271882 32.9317970637428 20.866381713436162 11 32.42955953571177 21.867113659930794 28.218927587834546 17.484399216522892 12 28.34432310677302 25.012823467316885 32.83328005709231 13.809573368817787 13 32.3267466583404 23.616129176145233 27.261102933523645 16.79602123199072 14 20.98280828919245 25.108818883003437 29.669733296162065 24.23863953164204 15 20.051218007329602 37.32075555852902 27.068605368598682 15.559421065542695 16 16.30120844686142 28.035892841875977 36.52252781360701 19.14037089765559 17 15.898598669293387 30.717392112403818 32.42946883247221 20.954540385830576 18 16.367218721537576 26.58822654696184 40.90559304076284 16.138961690737748 19 22.859628188114065 24.562929589192848 32.096562542982156 20.480879679710938 20 24.37100371747212 26.073234200743496 33.85724907063197 15.698513011152414 21 22.899023770046917 23.927671249172842 29.77241128054901 23.400893700231233 22 23.086702924546188 30.232082647147386 30.196766530730596 16.484447897575826 23 18.454091552934965 31.26094408155223 33.36889966205791 16.916064703454893 24 22.386367438281358 27.620509808440797 34.389579298372134 15.603543454905708 25 20.039778430424924 29.581768838990296 34.67117736718837 15.707275363396409 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 170.0 1 170.0 2 493.0 3 816.0 4 816.0 5 816.0 6 2425.5 7 4035.0 8 4035.0 9 4035.0 10 3458.0 11 2881.0 12 2881.0 13 2881.0 14 1947.0 15 1013.0 16 1013.0 17 1013.0 18 2043.0 19 3073.0 20 3073.0 21 3073.0 22 5618.0 23 8163.0 24 8163.0 25 8163.0 26 14097.0 27 20031.0 28 20031.0 29 20031.0 30 26786.5 31 33542.0 32 33542.0 33 33542.0 34 32608.0 35 31674.0 36 31674.0 37 31674.0 38 30196.5 39 28719.0 40 28719.0 41 28719.0 42 29423.0 43 30127.0 44 30127.0 45 30127.0 46 35447.0 47 40767.0 48 40767.0 49 40767.0 50 36150.0 51 31533.0 52 31533.0 53 31533.0 54 22221.5 55 12910.0 56 12910.0 57 12910.0 58 10348.5 59 7787.0 60 7787.0 61 7787.0 62 6519.5 63 5252.0 64 5252.0 65 5252.0 66 4304.5 67 3357.0 68 3357.0 69 3357.0 70 2535.0 71 1713.0 72 1713.0 73 1713.0 74 1309.5 75 906.0 76 906.0 77 906.0 78 625.5 79 345.0 80 345.0 81 345.0 82 243.0 83 141.0 84 141.0 85 141.0 86 103.5 87 66.0 88 66.0 89 66.0 90 48.5 91 31.0 92 31.0 93 31.0 94 22.5 95 14.0 96 14.0 97 14.0 98 84.5 99 155.0 100 155.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.039744299293145775 2 0.005571630742029782 3 0.0011143261484059565 4 0.004085862544155173 5 0.010771819434590912 6 0.018200660423963955 7 0.024886617314399697 8 0.03900141519420848 9 0.05163044487614264 10 0.06351659045913952 11 0.06091649611285895 12 0.06797389505276334 13 0.07168831554744987 14 0.07280264169585582 15 0.06500235865701412 16 0.07354552579479312 17 0.07094543144851256 18 0.08394590317991539 19 0.07948859858629155 20 0.08208869293257212 21 0.08431734522938404 22 0.08246013498204077 23 0.08914609187247652 24 0.08097436678416617 25 0.08580311342725865 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 269221.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.3918193603025 #Duplication Level Percentage of deduplicated Percentage of total 1 70.56701587644454 24.269280628182795 2 14.875256507182202 10.231742694663492 3 5.497353925909926 5.671920095386318 4 2.7735176584944377 3.8154527321419947 5 1.508802246462901 2.594522715538535 6 0.9353061885732801 1.9300128890391166 7 0.589696511502322 1.4196515130691887 8 0.4633329733232531 1.274789113776414 9 0.3380494653850308 1.0463522533531933 >10 2.0369370342369586 13.284624899246344 >50 0.2095258667242683 5.125528840617931 >100 0.16848471757209202 11.678880919393361 >500 0.019440544335241387 4.631139472775155 >1k 0.016200453612701157 11.076030473105739 >5k 0.0010800302408467436 1.9500707597104237 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 5250 1.9500707597104237 No Hit TATCAACGCAGAGTACTTTTTTTTT 3680 1.36690674204464 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 3559 1.321962254058933 No Hit GTACATGGGGTGGTATCAACGCAAA 2717 1.009208048406328 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 2195 0.8153152985836915 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2000 0.7428840989373042 No Hit GGTATCAACGCAGAGTACTTTTTTT 1975 0.733598047700588 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1965 0.7298836272059015 No Hit GTCCTACAGTGGACATTTCTAAATT 1884 0.6997968211989406 No Hit CTGTAGGACGTGGAATATGGCAAGA 1797 0.6674813628951679 No Hit CTTTAGGACGTGAAATATGGCGAGG 1671 0.6206796646621178 No Hit GTATCAACGCAGAGTACATGGGGTG 1633 0.6065648667823089 No Hit GTCCTACAGTGTGCATTTCTCATTT 1356 0.5036754190794923 No Hit GTACATGGGTGGTATCAACGCAAAA 1274 0.4732171710230628 No Hit TATCAACGCAGAGTACATGGGGTGG 1102 0.40932913851445474 No Hit CTGTAGGACCTGGAATATGGCGAGA 1011 0.37552791201280733 No Hit GTACATGGGAGTGGTATCAACGCAA 993 0.36884195512237156 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 975 0.36215599823193584 No Hit GGTATCAACGCAGAGTACATGGGGT 856 0.3179543943451662 No Hit ATTTAGAAATGTCCACTGTAGGACG 785 0.2915820088328919 No Hit CTGAAGGACCTGGAATATGGCGAGA 768 0.28526749399192486 No Hit GATATACACTGTTCTACAAATCCCG 729 0.2707812540626474 No Hit GTACATGGAAGCAGTGGTATCAACG 723 0.2685526017658355 No Hit GTATCAACGCAGAGTACATGGGAGT 651 0.24180877420409255 No Hit GTCCTTCAGTGTGCATTTCTCATTT 647 0.24032300600621792 No Hit ACGCAGAGTACATGGGGTGGTATCA 644 0.23920867985781197 No Hit GTATCAACGCAGAGTACATGGGAAG 630 0.23400849116525083 No Hit TTTCTAAATTTTCCACCTTTTTCAG 624 0.23177983886843895 No Hit ATCAACGCAGAGTACTTTTTTTTTT 616 0.22880830247268974 No Hit TCCATGTACTCTGCGTTGATACCAC 607 0.22546532402747185 No Hit GAATATGGCAAGAAAACTGAAAATC 601 0.22323667173065995 No Hit TTGTAGAACAGTGTATATCAATGAG 566 0.2102361999992571 No Hit CTGTAGGACATGGAATATGGCAAGA 535 0.1987214964657289 No Hit CCCATGTACTCTGCGTTGATACCAC 518 0.1924069816247618 No Hit GTACATGGGAAGCAGTGGTATCAAC 492 0.18274948833857688 No Hit GGAATATGGCGAGAAAACTGAAAAT 484 0.17977795194282764 No Hit GTGTATATCAATGAGTTACAATGAG 477 0.17717785759654708 No Hit GTATCAACGCAGAGTACATGGGTGG 450 0.16714892226089348 No Hit GTGTATATCAATGAGTTACAATGAA 438 0.16269161766726964 No Hit GGTATCAACGCAGAGTACATGGGAA 430 0.15972008127152043 No Hit TATCAACGCAGAGTACATGGGAGTG 403 0.14969114593586683 No Hit GATATACACTGTTCTACAATGCCGG 400 0.14857681978746085 No Hit GTTCTACAGTGTGGTTTTTATCATT 392 0.14560528339171164 No Hit GCCATATTCCACGTCCTACAGTGGA 385 0.14300518904543108 No Hit GAGTACATGGGGTGGTATCAACGCA 384 0.14263374699596243 No Hit GAAATATGGCGAGGAAAACTGAAAA 381 0.14151942084755648 No Hit GTCCACTGTAGGACGTGGAATATGG 375 0.13929076855074457 No Hit CATGTACTCTGCGTTGATACCACTG 343 0.12740462296774768 No Hit CCATATTCCAGGTCCTTCAGTGTGC 342 0.12703318091827903 No Hit GGTATCAACGCAGAGTACATGGGAG 342 0.12703318091827903 No Hit CCATATTTCACGTCCTAAAGTGTGT 341 0.12666173886881038 No Hit ACAGTGGACATTTCTAAATTTTCCA 337 0.12517597067093578 No Hit TATCAACGCAGAGTACATGGGAAGC 335 0.12443308657199847 No Hit ACCTGGAATATGGCGAGAAAACTGA 332 0.12331876042359251 No Hit GTTCTACAAATCCCGTTTCCAACGA 328 0.1218329922257179 No Hit GTATCAACGCAGAGTACATGGGCAG 324 0.12034722402784329 No Hit TATCAACGCAGAGTACATGGGTGGT 318 0.11811857173103138 No Hit ATTCCAGGTCCTTCAGTGTGCATTT 304 0.11291838303847027 No Hit GACCTGGAATATGGCGAGAAAACTG 303 0.1125469409890016 No Hit ATACACACTTTAGGACGTGAAATAT 301 0.1118040568900643 No Hit CTTTTTTTTTTTTTTTTTTTTTTTT 300 0.11143261484059565 No Hit TTCCAGGTCCTTCAGTGTGCATTTC 298 0.11068973074165835 No Hit CTGTAGAACATATTAGATGAGTGAG 294 0.10920396254378373 No Hit GTACATGGGGTATCAACGCAAAAAA 293 0.10883252049431508 No Hit ACTTTTTTTTTTTTTTTTTTTTTTT 292 0.10846107844484643 No Hit CAACGCAGAGTACTTTTTTTTTTTT 288 0.10697531024697182 No Hit CCTAAAGTGTGTATTTCTCATTTTC 284 0.1054895420490972 No Hit CACTTTAGGACGTGAAATATGGCGA 280 0.10400377385122259 No Hit GGTGGTATCAACGCAAAAAAAAAAA 279 0.10363233180175394 No Hit GGTATCAACGCAGAGTACATGGGTG 276 0.10251800565334798 No Hit GAAATACACACTTTAGGACGTGAAA 272 0.10103223745547339 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 3.7144204946865214E-4 0.0 13 0.0 0.0 0.0 3.7144204946865214E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCCTAG 30 7.6879107E-4 19.004461 1 TTTAAAC 30 7.716808E-4 18.993868 3 AAAGGTC 40 2.7455593E-4 16.628904 19 GAGGCAC 35 0.0021659143 16.283485 7 CCTAGAG 35 0.0021686058 16.280458 3 CCAACGT 60 1.4522739E-6 15.837051 19 TCCAACG 130 0.0 15.349758 18 TAGGACC 335 0.0 15.025001 4 TATGGTG 40 0.0052559054 14.253346 16 GTTCATG 40 0.0052559054 14.253346 1 GGCACTG 40 0.005268891 14.248049 9 ACACCAT 40 0.005268891 14.248049 6 AGGCACT 40 0.005268891 14.248049 8 CACCATT 40 0.005268891 14.248049 7 TCTACAC 60 2.5605113E-5 14.245401 3 CTACACC 60 2.5605113E-5 14.245401 4 GCCGGTT 75 9.582145E-7 13.934015 11 CCGGTTT 75 9.582145E-7 13.934015 12 TGCCGGT 75 9.582145E-7 13.934015 10 ATAGGAC 55 1.9560393E-4 13.813722 3 >>END_MODULE