##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064101_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	269221
Sequences flagged as poor quality	0
Sequence length	50
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.63864260217442	32.0	32.0	32.0	32.0	32.0
2	31.417181423440223	32.0	32.0	32.0	32.0	32.0
3	31.50252394872614	32.0	32.0	32.0	32.0	32.0
4	31.607720051556157	32.0	32.0	32.0	32.0	32.0
5	31.511962291203137	32.0	32.0	32.0	32.0	32.0
6	35.03670961774899	36.0	36.0	36.0	36.0	36.0
7	35.095215454960794	36.0	36.0	36.0	36.0	36.0
8	35.09359225320462	36.0	36.0	36.0	36.0	36.0
9	35.1774898689181	36.0	36.0	36.0	36.0	36.0
10	35.04443189795744	36.0	36.0	36.0	36.0	36.0
11	35.17411346068843	36.0	36.0	36.0	36.0	36.0
12	35.12781320922216	36.0	36.0	36.0	36.0	36.0
13	35.15358757303479	36.0	36.0	36.0	36.0	36.0
14	35.09956875578057	36.0	36.0	36.0	36.0	36.0
15	35.066150114589874	36.0	36.0	36.0	36.0	36.0
16	35.07660992270291	36.0	36.0	36.0	36.0	36.0
17	35.04495563124719	36.0	36.0	36.0	36.0	36.0
18	35.021513923505225	36.0	36.0	36.0	36.0	36.0
19	34.981238462081336	36.0	36.0	36.0	36.0	36.0
20	34.95648927832524	36.0	36.0	36.0	36.0	36.0
21	34.92658447892252	36.0	36.0	36.0	36.0	36.0
22	34.91251425408865	36.0	36.0	36.0	36.0	36.0
23	34.914118883742354	36.0	36.0	36.0	36.0	36.0
24	34.897589712541	36.0	36.0	36.0	36.0	36.0
25	34.864234216498716	36.0	36.0	36.0	36.0	36.0
26	34.84338517426204	36.0	36.0	36.0	32.0	36.0
27	34.779318849569684	36.0	36.0	36.0	32.0	36.0
28	34.715438245902064	36.0	36.0	36.0	32.0	36.0
29	34.67085034228385	36.0	36.0	36.0	32.0	36.0
30	34.59526188521698	36.0	36.0	36.0	32.0	36.0
31	34.51445095293458	36.0	36.0	36.0	32.0	36.0
32	34.4255128686098	36.0	36.0	36.0	32.0	36.0
33	34.3714123341047	36.0	36.0	36.0	32.0	36.0
34	34.26776885904145	36.0	36.0	36.0	32.0	36.0
35	34.16902470461071	36.0	36.0	36.0	32.0	36.0
36	34.06224997307045	36.0	36.0	36.0	32.0	36.0
37	33.933318723279385	36.0	36.0	36.0	32.0	36.0
38	33.839429316435194	36.0	36.0	36.0	32.0	36.0
39	33.60005720207562	36.0	36.0	36.0	21.0	36.0
40	33.59897259129117	36.0	36.0	36.0	21.0	36.0
41	33.53325706389918	36.0	36.0	36.0	21.0	36.0
42	33.42968416282534	36.0	36.0	36.0	21.0	36.0
43	33.34517366773023	36.0	36.0	36.0	14.0	36.0
44	33.1426746056214	36.0	36.0	36.0	14.0	36.0
45	32.92068597917696	36.0	36.0	36.0	14.0	36.0
46	32.62673788448895	36.0	36.0	36.0	14.0	36.0
47	32.47023820578632	36.0	36.0	36.0	14.0	36.0
48	32.233477329034514	36.0	36.0	36.0	14.0	36.0
49	32.06886535597149	36.0	36.0	36.0	14.0	36.0
50	31.635195619955354	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	4.0
21	11.0
22	47.0
23	138.0
24	383.0
25	841.0
26	1843.0
27	3452.0
28	5741.0
29	7961.0
30	10362.0
31	13479.0
32	18061.0
33	25759.0
34	45105.0
35	136033.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.948624412133995	19.696575703767543	13.392200420514575	21.962599463583885
2	11.200959816951318	22.292714454456984	47.33524504305061	19.171080685541085
3	19.244627030882036	30.896893966971007	29.941162923727237	19.91731607841972
4	9.69500893318129	17.850019129265547	38.521883508344445	33.933088429208716
5	8.965868190074326	43.24699781963517	34.1667254783245	13.620408511966007
6	27.066339796449	39.61629893767179	19.88856697125028	13.42879429462893
7	25.019222126060004	33.79974073344947	22.103030595681613	19.07800654480891
8	25.388435523231845	39.35465658325316	19.20503972572719	16.051868167787802
9	28.79732259598241	12.898193272316652	21.276224295732796	37.02825983596815
10	17.169601069757075	29.244855508506056	32.72453755293069	20.86100586880618
11	32.34703087797757	21.572239906990912	28.251139398486746	17.82958981654477
12	27.91508803209271	25.35101404056162	32.97526186761756	13.758636059728103
13	32.2799197682193	23.293217442983433	27.600475447589336	16.826387341207933
14	20.779954015474274	25.076795643727646	30.03591844618362	24.10733189461446
15	20.47479839084132	36.89475463301747	27.058530732627812	15.571916243513403
16	16.498402793254588	27.654334744818364	36.593863754550185	19.253398707376867
17	15.967922264607887	30.56708057692379	32.80019017832933	20.664806980138994
18	16.552695017810777	25.774183842893706	41.30689141553902	16.366229723756494
19	23.03432137285491	24.0405616224649	32.988633831067524	19.93648317361266
20	24.270023512281732	25.666645618283862	34.84720731295107	15.216123556483335
21	22.557304222181777	23.47625185256722	31.150244594589577	22.816199330661426
22	23.010092080484064	29.305663376928248	31.28730671084351	16.39693783174418
23	18.399821707154	30.17383552484956	34.4101478344848	17.016194933511628
24	22.42692806281828	26.75942812782064	35.23053550800272	15.583108301358365
25	20.043534140126443	28.966918510924394	35.217336394095405	15.77221095485376
26	15.52176933163453	30.928386495250905	37.29853013829404	16.25131403482053
27	16.71699893392172	30.70528321651047	35.70693619502918	16.870781654538632
28	15.711643041811534	31.39505512466197	33.36787911206205	19.525422721464444
29	18.047151554322348	27.720017680904252	32.66695639576995	21.565874369003442
30	16.100070576873073	29.053526986367515	34.826343746517594	20.020058690241818
31	20.463519823477625	27.683403850654344	33.06265578508253	18.79042054078551
32	16.743928621839874	31.877242639684418	33.57700565349499	17.80182308498072
33	16.011411250780068	32.14503283706279	35.477028914445334	16.366526997711805
34	17.663446216249344	31.10761950396892	35.390038519112835	15.838895760668903
35	16.703068934007888	32.868647247171396	33.947714457643364	16.480569361177352
36	15.617013372956908	31.86515601783061	35.20579494799406	17.312035661218424
37	16.50570186842985	32.183797035771335	30.92492849448386	20.38557260131496
38	17.85337513558491	35.421093297276414	30.329415610466416	16.396115956672265
39	19.859958024553777	30.551809958953214	31.467097565052654	18.12113445144035
40	21.54701057886604	31.191682515155115	32.104332580530134	15.15697432544871
41	20.336760619883215	29.377590894908103	33.883705035436755	16.401943449771927
42	18.188301630283377	35.80383111021963	31.482781547970596	14.525085711526389
43	15.424731721993782	35.496272523651925	32.59489556751617	16.484100186838127
44	15.040543200888495	35.05696106144068	31.35811365468262	18.5443820829882
45	16.124359260084688	35.02005794517495	30.49996285565708	18.35561993908328
46	17.6863445744914	30.80317511022625	30.493761584434974	21.016718730847376
47	21.903891656173272	31.521783542644037	28.83324604315478	17.74107875802791
48	17.225753011488788	33.66181435931342	27.94527875075682	21.167153878440974
49	20.054973627516528	32.051110615853204	28.66020355099918	19.233712205631083
50	16.891698641636424	36.921339717184026	26.885718424639983	19.30124321653957
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	32.0
1	33.0
2	34.0
3	80.5
4	127.0
5	180.0
6	233.0
7	234.0
8	235.0
9	349.0
10	463.0
11	715.0
12	967.0
13	1712.0
14	2457.0
15	3547.0
16	4637.0
17	5566.5
18	6496.0
19	6614.0
20	6732.0
21	6640.5
22	6549.0
23	6626.5
24	6704.0
25	5512.0
26	4320.0
27	5370.5
28	6421.0
29	8113.0
30	9805.0
31	12294.0
32	14783.0
33	19189.0
34	23595.0
35	27402.5
36	31210.0
37	29647.0
38	28084.0
39	24206.5
40	20329.0
41	17614.5
42	14900.0
43	12765.0
44	10630.0
45	10548.5
46	10467.0
47	10728.0
48	10989.0
49	10139.5
50	9290.0
51	8490.0
52	7690.0
53	7098.5
54	6507.0
55	6045.0
56	5583.0
57	5316.5
58	5050.0
59	4513.0
60	3976.0
61	3461.5
62	2947.0
63	2522.0
64	2097.0
65	1828.0
66	1559.0
67	1365.5
68	1172.0
69	1011.5
70	851.0
71	688.0
72	525.0
73	443.0
74	361.0
75	270.5
76	180.0
77	145.0
78	110.0
79	88.0
80	66.0
81	45.0
82	24.0
83	16.0
84	8.0
85	6.0
86	4.0
87	4.0
88	4.0
89	2.0
90	0.0
91	0.5
92	1.0
93	4.0
94	7.0
95	5.0
96	3.0
97	4.5
98	6.0
99	3.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.010028935335653608
2	0.0011143261484059565
3	0.0011143261484059565
4	0.0
5	0.0
6	3.7144204946865214E-4
7	0.0
8	0.0
9	0.0018572102473432607
10	3.7144204946865214E-4
11	0.0
12	3.7144204946865214E-4
13	3.7144204946865214E-4
14	0.0
15	0.004457304593623826
16	3.7144204946865214E-4
17	0.0
18	7.428840989373043E-4
19	3.7144204946865214E-4
20	0.0
21	0.0
22	0.0
23	3.7144204946865214E-4
24	0.0
25	0.0026000943462805653
26	0.004457304593623826
27	0.0037144204946865213
28	0.004828746643092478
29	0.0014857681978746086
30	0.004085862544155173
31	0.007428840989373043
32	0.0026000943462805653
33	0.004828746643092478
34	0.0014857681978746086
35	0.0026000943462805653
36	0.007800283038841695
37	0.004085862544155173
38	0.006314514840967087
39	0.005943072791498434
40	0.0018572102473432607
41	0.0033429784452178694
42	0.0014857681978746086
43	0.0014857681978746086
44	7.428840989373043E-4
45	3.7144204946865214E-4
46	7.428840989373043E-4
47	0.0014857681978746086
48	7.428840989373043E-4
49	3.7144204946865214E-4
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	269221.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.40254819701631
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.68876405461896	38.14906578235527
2	13.05536944224119	13.160477750848312
3	4.537314666424789	6.860766634785135
4	2.166521961679988	4.367929103738797
5	1.1952308918735255	3.0121341317109076
6	0.7197816166346571	2.1767296574252772
7	0.503806918336081	1.7775206738397207
8	0.36429682788055096	1.4689190740215698
9	0.26103227325207257	1.1841022560207888
>10	1.3198574824673635	12.056471191457169
>50	0.10322878840284544	3.7610213647053885
>100	0.07447219734776707	7.334734946030193
>500	0.007373484885917532	2.70890165289897
>1k	0.0029493939543670127	1.9812257801624926
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1926	0.715397387276624	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA	1252	0.46504544593475244	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1132	0.4204723999985142	No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	1021	0.37924233250749384	No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	979	0.36364176642981044	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	853	0.31684006819676025	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	781	0.2900962406350173	No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA	767	0.2848960519424562	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA	745	0.27672432685414583	No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA	699	0.25963799257858783	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA	678	0.25183770953974616	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC	632	0.23475137526418813	No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA	609	0.22620820812640918	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	546	0.2028073590098841	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	460	0.17086334275558	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	454	0.16863469045876808	No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAA	435	0.16157729151886369	No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA	411	0.15266268233161603	No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG	382	0.14189086289702513	No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA	375	0.13929076855074457	No Hit
TATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA	344	0.12777606501721633	No Hit
GAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA	340	0.12629029681934173	No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTCA	339	0.12591885476987308	No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	329	0.12220443427518655	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	317	0.11774712968156273	No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA	313	0.11626136148368811	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG	309	0.11477559328581352	No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCAGTGATTTCGTCA	307	0.11403270918687622	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	307	0.11403270918687622	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT	301	0.1118040568900643	No Hit
ATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	282	0.1047466579501599	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	281	0.10437521590069125	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	275	0.10214656360387935	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT	274	0.1017751215544107	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	3.7144204946865214E-4	0.0
13	0.0	0.0	0.0	3.7144204946865214E-4	0.0
14	0.0	0.0	0.0	7.428840989373043E-4	0.0
15	0.0	0.0	0.0	7.428840989373043E-4	0.0
16	0.0	0.0	0.0	0.0011143261484059565	0.0
17	3.7144204946865214E-4	0.0	0.0	0.0014857681978746086	0.0
18	3.7144204946865214E-4	0.0	0.0	0.0014857681978746086	0.0
19	3.7144204946865214E-4	0.0	0.0	0.002228652296811913	0.0
20	3.7144204946865214E-4	0.0	0.0	0.002228652296811913	0.0
21	3.7144204946865214E-4	0.0	0.0	0.002600094346280565	0.0
22	3.7144204946865214E-4	0.0	0.0	0.004457304593623826	0.0
23	3.7144204946865214E-4	0.0	0.0	0.013000471731402825	0.0
24	3.7144204946865214E-4	0.0	0.0	0.027858153710148912	0.0
25	3.7144204946865214E-4	0.0	0.0	0.03305834240271004	0.0
26	3.7144204946865214E-4	0.0	0.0	0.041230067491020386	0.0
27	3.7144204946865214E-4	0.0	0.0	0.049030350529862085	0.0
28	3.7144204946865214E-4	0.0	0.0	0.05905928586551569	0.0
29	3.7144204946865214E-4	0.0	0.0	0.07874571448735425	0.0
30	3.7144204946865214E-4	0.0	0.0	0.10660386819750317	0.0
31	3.7144204946865214E-4	0.0	0.0	0.1838638144869828	0.0
32	3.7144204946865214E-4	0.0	0.0	0.2607523187269938	0.0
33	3.7144204946865214E-4	0.0	0.0	0.314982857949417	0.0
34	3.7144204946865214E-4	0.0	0.0	0.36809907102343425	0.0
35	3.7144204946865214E-4	0.0	0.0	0.4449875752634453	0.0
36	3.7144204946865214E-4	0.0	0.0	0.5423053922242321	0.0
37	3.7144204946865214E-4	0.0	0.0	0.6719386674887917	0.0
38	3.7144204946865214E-4	0.0	0.0	0.8246013498204078	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCAATG	20	7.846056E-4	43.999626	2
TAGTACA	20	7.846056E-4	43.999626	26
CGGAAAT	25	0.0023496537	35.1997	39
GATTCCT	25	0.0023496537	35.1997	39
ATACAGC	25	0.0023496537	35.1997	15
CCTACAT	40	1.8149529E-5	32.99972	3
TGGGGGA	40	1.8149529E-5	32.99972	6
ATACATG	50	2.3458051E-6	30.811184	1
GTTCTAA	45	4.047896E-5	29.343988	1
CTATGTT	30	0.005737422	29.333086	9
CCAGGAT	30	0.005737422	29.333086	3
AGCGAGA	30	0.005737422	29.333086	19
ACTAAGG	30	0.005737422	29.333086	3
GACATGT	30	0.005737422	29.333086	7
GGGTTCG	30	0.005737422	29.333086	33
TAGGACC	375	0.0	29.333086	4
CCTATGG	30	0.005737422	29.333086	3
AATACTT	90	1.6370905E-11	29.333086	5
ATAAGAG	40	7.018192E-4	27.499767	9
TAGGGGG	40	7.018192E-4	27.499767	29
>>END_MODULE