FastQCFastQC Report
Thu 2 Feb 2017
SRR4064100_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064100_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences896295
Sequences flagged as poor quality0
Sequence length25
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA20690.23083917683352023No Hit
GTCCTAAAGTGTGTATTTCTCATTT20310.22659950128027045No Hit
GTCCTACAGTGGACATTTCTAAATT18370.2049548418768374No Hit
CCATAGGGTCTTCTCGTCTTATTAT18130.20227715205373228No Hit
GTATTAGAGGCACTGCCTGCCCAGT17690.19736805404470628No Hit
CTTTAGGACGTGAAATATGGCGAGG17470.19491350504019325No Hit
GTCCTACAGTGTGCATTTCTCATTT14230.15876469242827418No Hit
GTATCAACGCAGAGTACTTTTTTTT13640.15218203827980745No Hit
CCATTGGGATGTCCTGATCCAACAT12660.1412481381687949No Hit
CTCTAATACTTGTAATGCTAGAGGT12380.13812416670850558No Hit
CTTCTACACCATTGGGATGTCCTGA10590.11815306344451323No Hit
GTATTGGAATTAGTGAAATTGGAGT10570.11792992262592114No Hit
GCCTAAAGGAAAGATCCAAAAAGAT10480.11692578894225675No Hit
GATATATTTTGATCAACGGACCAAG10380.11581008484929628No Hit
CCTCTAGCATTACAAGTATTAGAGG10360.11558694403070419No Hit
GTATCAACGCAGAGTACATGGGAGA10140.11313239502619116No Hit
ATTTAGAAATGTCCACTGTAGGACG10050.11212826134252674No Hit
GTCAGGATACCGCGGCCGTTAAACT9610.10721916333350069No Hit
TATCAACGCAGAGTACTTTTTTTTT9420.1050993255568758No Hit
ATCCTGACCGTGCAAAGGTAGCATA9390.10476461432898766No Hit
ATCGTAAATAGATAGAAACCGACCT9110.10164064286869835No Hit
CCTATAACTTCTCTGTTAACCCAAC9100.1015290724594023No Hit
TGCTAGAGGTGATGTTTTTGGTAAA9060.10108279082221812No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTTAC250.006026442619.0020081
CCGCCCA250.006026442619.00200811
CTGGTCG401.2842818E-518.9998879
ATTCCGC350.00216931916.285628
GGTATCA4600.015.6973121
GTCTAAT456.752953E-414.7793411
TGGTCGG456.755813E-414.77851610
GTCGGTT456.755813E-414.77851612
ACTAGGC400.00527699914.2499168
AAGTTAG400.00527699914.2499167
TATTCCG400.00527699914.2499167
CGACATG551.9569354E-413.81964515
ATAGGAC551.9615755E-413.8157863
TCCGGTG1051.366061E-913.57210712
CATTCCG1051.36788E-913.571359
ATCGCAG500.001497487913.30140716
CGTCTGC500.001498118213.30066410
AGAACAG2050.012.9740855
CGTCTTA3050.012.77184315
TCCAACG1207.385097E-1012.66729918
Produced by FastQC (version 0.11.5)