##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064100_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	896295
Sequences flagged as poor quality	0
Sequence length	25
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.338477844905974	32.0	32.0	32.0	32.0	32.0
2	30.994566521067284	32.0	32.0	32.0	32.0	32.0
3	31.011716008680178	32.0	32.0	32.0	32.0	32.0
4	31.04283857435331	32.0	32.0	32.0	32.0	32.0
5	31.006254637145137	32.0	32.0	32.0	32.0	32.0
6	34.66602290540503	36.0	36.0	36.0	32.0	36.0
7	34.59328346136038	36.0	36.0	36.0	32.0	36.0
8	34.56126833241288	36.0	36.0	36.0	32.0	36.0
9	34.66676038581048	36.0	36.0	36.0	32.0	36.0
10	34.435863192364124	36.0	36.0	36.0	32.0	36.0
11	34.67141175617403	36.0	36.0	36.0	32.0	36.0
12	34.50285787603412	36.0	36.0	36.0	32.0	36.0
13	34.5779693069804	36.0	36.0	36.0	32.0	36.0
14	34.48104809242493	36.0	36.0	36.0	32.0	36.0
15	34.45080023876068	36.0	36.0	36.0	32.0	36.0
16	34.45354375512527	36.0	36.0	36.0	32.0	36.0
17	34.409476790565606	36.0	36.0	36.0	32.0	36.0
18	34.40736810982991	36.0	36.0	36.0	32.0	36.0
19	34.391624409374145	36.0	36.0	36.0	32.0	36.0
20	34.38135435319845	36.0	36.0	36.0	32.0	36.0
21	34.3720426868386	36.0	36.0	36.0	32.0	36.0
22	34.35133187176097	36.0	36.0	36.0	32.0	36.0
23	34.31950083398881	36.0	36.0	36.0	32.0	36.0
24	34.29853117556162	36.0	36.0	36.0	32.0	36.0
25	33.95564629948845	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	6.0
5	11.0
6	34.0
7	17.0
8	58.0
9	74.0
10	65.0
11	27.0
12	61.0
13	58.0
14	72.0
15	113.0
16	229.0
17	276.0
18	325.0
19	413.0
20	671.0
21	965.0
22	1508.0
23	2382.0
24	3648.0
25	5623.0
26	8114.0
27	10918.0
28	15459.0
29	20663.0
30	27786.0
31	38175.0
32	55102.0
33	78673.0
34	180683.0
35	444085.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.21527046091755	22.6561244352376	13.446032823186957	24.68257228065789
2	13.20594299792022	22.459743285340664	43.11407110531906	21.220242611420055
3	15.937006644092003	27.866024757750047	32.09035072549468	24.10661787266327
4	9.962745559647828	19.332477191557444	43.146533016315416	27.55824423247931
5	10.61307833499774	40.405371478941994	37.97046467644483	11.01108550961544
6	26.444894010419862	43.13821103737783	19.629357278114828	10.787537674087481
7	25.723203903214216	33.86911019304037	24.329916235344534	16.077769668400883
8	23.236699770749205	38.51915594634594	21.92202347867432	16.322120804230536
9	25.522447872422365	16.40264737433735	22.708747520458658	35.36615723278163
10	13.912301815007243	28.677404733888988	36.80095204710222	20.609341404001544
11	30.850812051496167	24.58348032276647	27.995346947159717	16.570360678577646
12	21.70656877587385	28.0840958722418	32.63320427111105	17.5761310807733
13	26.229822998376683	23.810927343814544	28.322939204955244	21.63631045285353
14	19.442363368194925	24.760812695321878	29.421182912273615	26.375641024209585
15	21.80918346043793	33.585421320749035	24.334640662975776	20.270754555837268
16	20.808412148847157	29.877620562335807	28.951931375515404	20.362035913301632
17	18.790003405138968	31.22601749459923	29.305407472326266	20.67857162793554
18	18.877900244648384	29.78010676987084	31.869276696096684	19.47271628938409
19	21.955756639183914	29.04481058339065	29.18326648741838	19.816166290007057
20	21.656343274832253	29.941409586852163	29.38051832471841	19.021728813597175
21	21.90024463745979	28.3816299710477	27.999656100695503	21.71846929079701
22	21.380101992391324	30.30739528583887	28.52492917815393	19.78757354361587
23	20.632370019653386	30.626786671431123	29.42257012685367	19.31827318206182
24	21.448239668590954	29.63855421686747	29.443147939301227	19.47005817524035
25	20.444280177453013	29.81840432979998	30.319096074974233	19.418219417772768
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	46.0
1	46.0
2	141.0
3	236.0
4	236.0
5	236.0
6	871.0
7	1506.0
8	1506.0
9	1506.0
10	1936.5
11	2367.0
12	2367.0
13	2367.0
14	4250.0
15	6133.0
16	6133.0
17	6133.0
18	11960.0
19	17787.0
20	17787.0
21	17787.0
22	25445.5
23	33104.0
24	33104.0
25	33104.0
26	50450.5
27	67797.0
28	67797.0
29	67797.0
30	83389.0
31	98981.0
32	98981.0
33	98981.0
34	109890.5
35	120800.0
36	120800.0
37	120800.0
38	122282.5
39	123765.0
40	123765.0
41	123765.0
42	117494.5
43	111224.0
44	111224.0
45	111224.0
46	112741.0
47	114258.0
48	114258.0
49	114258.0
50	99495.0
51	84732.0
52	84732.0
53	84732.0
54	69070.0
55	53408.0
56	53408.0
57	53408.0
58	41082.0
59	28756.0
60	28756.0
61	28756.0
62	22254.5
63	15753.0
64	15753.0
65	15753.0
66	11820.5
67	7888.0
68	7888.0
69	7888.0
70	5692.0
71	3496.0
72	3496.0
73	3496.0
74	2762.0
75	2028.0
76	2028.0
77	2028.0
78	1481.0
79	934.0
80	934.0
81	934.0
82	710.5
83	487.0
84	487.0
85	487.0
86	355.0
87	223.0
88	223.0
89	223.0
90	152.0
91	81.0
92	81.0
93	81.0
94	65.5
95	50.0
96	50.0
97	50.0
98	252.5
99	455.0
100	455.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03826865038854395
2	0.005243809236914186
3	0.0011157040929604652
4	0.0029008306416972093
5	0.008925632743683722
6	0.013611589934117674
7	0.023764497180057905
8	0.03715294629558349
9	0.051768669913365574
10	0.060359591429161155
11	0.06013645061056906
12	0.06727695680551604
13	0.06660753434973976
14	0.0734133293167986
15	0.06426455575452278
16	0.07118192113087768
17	0.06582654148466743
18	0.08077697633033766
19	0.07843399773512069
20	0.08434722942781116
21	0.07631415995849582
22	0.08233896206048232
23	0.08646706720443603
24	0.08088854673963371
25	0.08189268042329813
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	896295.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.716376606525174
#Duplication Level	Percentage of deduplicated	Percentage of total
1	59.30375933626881	16.4368532794676
2	16.08073743440192	8.913995496850621
3	7.794482504447404	6.481044376387074
4	4.514009962188177	5.0044800047045594
5	2.876562390424537	3.986394327258638
6	1.930641011577005	3.21062240213226
7	1.3110736705542978	2.5436748128387565
8	0.9773489618110429	2.1670857521241005
9	0.7000506738893512	1.7462581309052099
>10	3.797293063216939	19.179333639708464
>50	0.38731070570863374	7.526147957488008
>100	0.29603621666281343	15.597656945318466
>500	0.02382829029073099	4.54076268452326
>1k	0.006865778558346218	2.665690190293034
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTAGGACGTGGAATATGGCAAGA	2069	0.23083917683352023	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	2031	0.22659950128027045	No Hit
GTCCTACAGTGGACATTTCTAAATT	1837	0.2049548418768374	No Hit
CCATAGGGTCTTCTCGTCTTATTAT	1813	0.20227715205373228	No Hit
GTATTAGAGGCACTGCCTGCCCAGT	1769	0.19736805404470628	No Hit
CTTTAGGACGTGAAATATGGCGAGG	1747	0.19491350504019325	No Hit
GTCCTACAGTGTGCATTTCTCATTT	1423	0.15876469242827418	No Hit
GTATCAACGCAGAGTACTTTTTTTT	1364	0.15218203827980745	No Hit
CCATTGGGATGTCCTGATCCAACAT	1266	0.1412481381687949	No Hit
CTCTAATACTTGTAATGCTAGAGGT	1238	0.13812416670850558	No Hit
CTTCTACACCATTGGGATGTCCTGA	1059	0.11815306344451323	No Hit
GTATTGGAATTAGTGAAATTGGAGT	1057	0.11792992262592114	No Hit
GCCTAAAGGAAAGATCCAAAAAGAT	1048	0.11692578894225675	No Hit
GATATATTTTGATCAACGGACCAAG	1038	0.11581008484929628	No Hit
CCTCTAGCATTACAAGTATTAGAGG	1036	0.11558694403070419	No Hit
GTATCAACGCAGAGTACATGGGAGA	1014	0.11313239502619116	No Hit
ATTTAGAAATGTCCACTGTAGGACG	1005	0.11212826134252674	No Hit
GTCAGGATACCGCGGCCGTTAAACT	961	0.10721916333350069	No Hit
TATCAACGCAGAGTACTTTTTTTTT	942	0.1050993255568758	No Hit
ATCCTGACCGTGCAAAGGTAGCATA	939	0.10476461432898766	No Hit
ATCGTAAATAGATAGAAACCGACCT	911	0.10164064286869835	No Hit
CCTATAACTTCTCTGTTAACCCAAC	910	0.1015290724594023	No Hit
TGCTAGAGGTGATGTTTTTGGTAAA	906	0.10108279082221812	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.1157040929604651E-4	0.0
2	0.0	0.0	0.0	1.1157040929604651E-4	0.0
3	0.0	0.0	0.0	2.2314081859209302E-4	0.0
4	0.0	0.0	0.0	3.347112278881395E-4	0.0
5	0.0	0.0	0.0	3.347112278881395E-4	0.0
6	0.0	0.0	0.0	3.347112278881395E-4	0.0
7	0.0	0.0	0.0	4.4628163718418603E-4	0.0
8	0.0	0.0	0.0	4.4628163718418603E-4	0.0
9	0.0	0.0	0.0	4.4628163718418603E-4	0.0
10	0.0	0.0	0.0	4.4628163718418603E-4	0.0
11	0.0	0.0	0.0	5.578520464802325E-4	0.0
12	0.0	0.0	0.0	5.578520464802325E-4	0.0
13	0.0	0.0	0.0	5.578520464802325E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCTTAC	25	0.0060264426	19.002008	1
CCGCCCA	25	0.0060264426	19.002008	11
CTGGTCG	40	1.2842818E-5	18.999887	9
ATTCCGC	35	0.002169319	16.28562	8
GGTATCA	460	0.0	15.697312	1
GTCTAAT	45	6.752953E-4	14.779341	1
TGGTCGG	45	6.755813E-4	14.778516	10
GTCGGTT	45	6.755813E-4	14.778516	12
ACTAGGC	40	0.005276999	14.249916	8
AAGTTAG	40	0.005276999	14.249916	7
TATTCCG	40	0.005276999	14.249916	7
CGACATG	55	1.9569354E-4	13.819645	15
ATAGGAC	55	1.9615755E-4	13.815786	3
TCCGGTG	105	1.366061E-9	13.572107	12
CATTCCG	105	1.36788E-9	13.57135	9
ATCGCAG	50	0.0014974879	13.301407	16
CGTCTGC	50	0.0014981182	13.300664	10
AGAACAG	205	0.0	12.974085	5
CGTCTTA	305	0.0	12.771843	15
TCCAACG	120	7.385097E-10	12.667299	18
>>END_MODULE