FastQCFastQC Report
Thu 2 Feb 2017
SRR4064100_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064100_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences896295
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG17460.1948019346308972No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTAT15660.1747192609576088No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGA12060.13455391361103208No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT11550.1288638227369337No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA11060.12339687268142743No Hit
GCCTAAAGGAAAGATCCAAAAAGATAAAAGGAACTCGGCAAACAAGAACC10660.11893405630958556No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT10240.11424809911915162No Hit
GATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGCAATC9940.11090098684027022No Hit
ACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATC9770.10900428988223744No Hit
CCTCTAGCATTACAAGTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTT9680.10800015619857302No Hit
CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTA9630.10744230415209279No Hit
ATCGTAAATAGATAGAAACCGACCTGGATTGCTCCGGTCTGAACTCAGAT9250.10320262859884301No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTGCG207.853541E-444.0022926
ATCCGGT300.005742771629.33485823
AGAACGT300.00574590429.3315855
GCGATAT300.00574590429.33158513
CTAGGTA609.745372E-625.66800123
GTATCAA13900.025.638761
GGTATCA5350.024.6714271
AATCGGT500.002578307622.0023730
CTGTAGG13500.021.3468761
TAGGACG13650.021.2734574
TAGGACC4350.021.2401124
TTAGGAC7900.020.884833
GGACGTG13400.020.8495036
TATGCAC654.932776E-420.3064823
ATATCGT654.932776E-420.30648211
CTCTAGG1950.020.3064821
TGTAGGA14400.020.0126972
ACCGGTA550.00448137420.0010434
GACCGTT550.00448426919.9988087
GACGTGA6650.019.8484427
Produced by FastQC (version 0.11.5)