##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064100_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	896295
Sequences flagged as poor quality	0
Sequence length	50
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.40613525680719	32.0	32.0	32.0	32.0	32.0
2	31.51844872502915	32.0	32.0	32.0	32.0	32.0
3	31.559553495221998	32.0	32.0	32.0	32.0	32.0
4	31.619197920327572	32.0	32.0	32.0	32.0	32.0
5	31.607416085105907	32.0	32.0	32.0	32.0	32.0
6	35.179183193033545	36.0	36.0	36.0	36.0	36.0
7	35.199260288186366	36.0	36.0	36.0	36.0	36.0
8	35.158138782432125	36.0	36.0	36.0	36.0	36.0
9	35.24563341310618	36.0	36.0	36.0	36.0	36.0
10	35.149519968314	36.0	36.0	36.0	36.0	36.0
11	35.26738406439844	36.0	36.0	36.0	36.0	36.0
12	35.18247228869959	36.0	36.0	36.0	36.0	36.0
13	35.229205786041426	36.0	36.0	36.0	36.0	36.0
14	35.18340836443358	36.0	36.0	36.0	36.0	36.0
15	35.16983136132635	36.0	36.0	36.0	36.0	36.0
16	35.1754020718625	36.0	36.0	36.0	36.0	36.0
17	35.160325562454325	36.0	36.0	36.0	36.0	36.0
18	35.16657238967081	36.0	36.0	36.0	36.0	36.0
19	35.152776708561355	36.0	36.0	36.0	36.0	36.0
20	35.14628554214851	36.0	36.0	36.0	36.0	36.0
21	35.13784970350164	36.0	36.0	36.0	36.0	36.0
22	35.12877568211359	36.0	36.0	36.0	36.0	36.0
23	35.101955271422916	36.0	36.0	36.0	36.0	36.0
24	35.07477002549384	36.0	36.0	36.0	36.0	36.0
25	35.05647247836929	36.0	36.0	36.0	36.0	36.0
26	35.026175533724945	36.0	36.0	36.0	36.0	36.0
27	34.9871470888491	36.0	36.0	36.0	36.0	36.0
28	34.98400303471513	36.0	36.0	36.0	36.0	36.0
29	34.945871615930024	36.0	36.0	36.0	36.0	36.0
30	34.92657551364227	36.0	36.0	36.0	36.0	36.0
31	34.92025058713928	36.0	36.0	36.0	36.0	36.0
32	34.90260126409274	36.0	36.0	36.0	36.0	36.0
33	34.910962350565384	36.0	36.0	36.0	36.0	36.0
34	34.902218577588854	36.0	36.0	36.0	36.0	36.0
35	34.87391316475045	36.0	36.0	36.0	36.0	36.0
36	34.83198277352881	36.0	36.0	36.0	36.0	36.0
37	34.812052951316254	36.0	36.0	36.0	36.0	36.0
38	34.80187884569254	36.0	36.0	36.0	36.0	36.0
39	34.748273726842164	36.0	36.0	36.0	32.0	36.0
40	34.75635477158748	36.0	36.0	36.0	32.0	36.0
41	34.7405976826826	36.0	36.0	36.0	32.0	36.0
42	34.731049487055046	36.0	36.0	36.0	32.0	36.0
43	34.715989713208266	36.0	36.0	36.0	32.0	36.0
44	34.665106912344704	36.0	36.0	36.0	32.0	36.0
45	34.62591111185491	36.0	36.0	36.0	32.0	36.0
46	34.56409552658444	36.0	36.0	36.0	32.0	36.0
47	34.55997969418551	36.0	36.0	36.0	32.0	36.0
48	34.53323403566906	36.0	36.0	36.0	32.0	36.0
49	34.51678074741017	36.0	36.0	36.0	32.0	36.0
50	33.953968280532635	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	5.0
21	15.0
22	49.0
23	171.0
24	448.0
25	1153.0
26	2481.0
27	4575.0
28	8123.0
29	12926.0
30	19074.0
31	27230.0
32	39843.0
33	63250.0
34	135063.0
35	581887.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.49430935675647	23.50715458911324	13.92065234856194	25.07788370556835
2	12.764326605205698	23.187327273518417	42.41108037938741	21.63726574188848
3	15.316402876734642	28.35799414031557	32.3960664692612	23.92953651368859
4	9.79030341572808	19.951913153593402	43.0506697013818	27.207113729296715
5	10.27061402774756	40.83421195030654	37.806302612421135	11.088871409524765
6	26.46006779025632	43.34080112106072	19.46258705290898	10.736544035773976
7	25.645797421607842	33.96102845603289	24.408481582514685	15.984692539844584
8	23.047434159512214	38.86108926190596	21.79628358966635	16.29519298891548
9	25.423583202608107	16.14949312827882	22.73480891058841	35.69211475852466
10	13.735697908589344	28.926959616639795	36.715888361402904	20.621454113367957
11	30.845090065212904	24.72299856632024	27.929308988670027	16.50260237979683
12	21.548247165262175	28.17946403567591	32.523923591779905	17.74836520728201
13	26.235364735231965	23.77311462533499	28.322291569507325	21.669229069925716
14	19.386607519407693	24.733736921144178	29.371389298600683	26.508266260847446
15	21.738553150847252	33.62763333039517	24.213529468865307	20.420284049892274
16	20.977274071198018	29.86402823196536	28.68907238625042	20.469625310586206
17	18.65747326494067	31.29605765958752	29.18670750143647	20.859761574035335
18	18.854834930658605	29.82371776991822	31.811355699606153	19.510091599817024
19	21.955606134141103	29.031624632514962	29.201211654644954	19.811557578698977
20	21.619604303503433	29.992759064279202	29.308942499829854	19.07869413238751
21	21.89134157838658	28.216491222198048	28.038313278552263	21.85385392086311
22	21.39264571669564	30.32252809903893	28.4447960155931	19.84003016867233
23	20.56720734229922	30.536235085675777	29.40121791481737	19.495339657207634
24	21.54329858259845	29.826350458781103	29.17466260844728	19.45568835017316
25	20.5280516269542	29.739388313421312	30.240461643511594	19.492098416112892
26	19.389920601978925	30.263856717590297	30.76171221229933	19.584510468131448
27	19.871597466761283	31.136833273081884	28.804097991172046	20.187471268984787
28	19.772945350679493	29.407426416441655	29.774842121705753	21.044786111173096
29	20.476281859605745	29.13912678683798	29.18018556783848	21.204405785717793
30	20.46345874694983	28.941457901214047	29.574420843928273	21.020662507907854
31	21.832313868662006	27.994961069913483	29.625806998712374	20.54691806271214
32	19.423459488528657	30.42798152803207	29.65043173036907	20.4981272530702
33	19.661703421459293	30.693608403858274	29.892162386820715	19.752525787861714
34	20.273128932922745	29.38010443165082	31.15644664613737	19.190319989289062
35	20.312914908477467	30.543511975025716	29.11483216219895	20.028740954297866
36	19.732544840860275	29.212976652068395	30.23269826215515	20.821780244916177
37	21.153994204813106	28.460124896653987	28.162109028508436	22.22377187002447
38	20.115149013088157	30.656304743202078	29.29236914631289	19.93617709739687
39	20.807736165255985	29.659874723572898	28.312048539594624	21.220340571576486
40	21.185502712328095	29.38786849615856	30.20379837505774	19.222830416455608
41	21.67191436470869	28.071242242816307	30.029031559827107	20.227811832647895
42	20.10130762708082	30.49772392555898	29.253581470076316	20.147386977283883
43	19.163537976625477	30.599871691166214	29.80234860952275	20.43424172268556
44	19.17213832653676	29.60096397909147	30.11263158481956	21.114266109552208
45	19.757822027194536	29.95058480412995	29.870141462015905	20.421451706659617
46	20.803622930499134	28.336555873036772	29.379966997325646	21.479854199138448
47	21.603142778404443	29.137978549042316	28.866299146357655	20.392579526195583
48	19.73376653080898	30.040244075239798	28.85769083579815	21.36829855815308
49	20.843830261052734	28.147125831479404	29.829832621885227	21.17921128558263
50	18.973030990038918	29.930558034917347	29.686216930272412	21.410194044771323
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3.0
1	7.0
2	11.0
3	27.5
4	44.0
5	60.5
6	77.0
7	74.0
8	71.0
9	108.5
10	146.0
11	203.0
12	260.0
13	549.0
14	838.0
15	1118.5
16	1399.0
17	2065.5
18	2732.0
19	3024.0
20	3316.0
21	4403.5
22	5491.0
23	6829.5
24	8168.0
25	12256.5
26	16345.0
27	22309.0
28	28273.0
29	33541.0
30	38809.0
31	41952.5
32	45096.0
33	53905.5
34	62715.0
35	71700.0
36	80685.0
37	85092.0
38	89499.0
39	88893.0
40	88287.0
41	86428.0
42	84569.0
43	74896.5
44	65224.0
45	62283.0
46	59342.0
47	54857.5
48	50373.0
49	44346.5
50	38320.0
51	36101.0
52	33882.0
53	30096.5
54	26311.0
55	22985.0
56	19659.0
57	17484.0
58	15309.0
59	12399.5
60	9490.0
61	8055.5
62	6621.0
63	5609.0
64	4597.0
65	3847.0
66	3097.0
67	2759.5
68	2422.0
69	1979.5
70	1537.0
71	1359.0
72	1181.0
73	950.5
74	720.0
75	640.5
76	561.0
77	470.0
78	379.0
79	275.5
80	172.0
81	143.5
82	115.0
83	89.5
84	64.0
85	48.0
86	32.0
87	25.5
88	19.0
89	16.5
90	14.0
91	9.5
92	5.0
93	6.0
94	7.0
95	5.0
96	3.0
97	3.5
98	4.0
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009706625608756046
2	8.925632743683721E-4
3	1.1157040929604651E-4
4	0.0
5	0.0
6	1.1157040929604651E-4
7	0.0
8	0.0
9	0.0014504153208486047
10	0.0011157040929604652
11	0.0
12	4.4628163718418603E-4
13	1.1157040929604651E-4
14	1.1157040929604651E-4
15	0.004909098009026046
16	6.694224557762789E-4
17	0.0
18	5.578520464802326E-4
19	0.0
20	2.2314081859209302E-4
21	0.0
22	1.1157040929604651E-4
23	0.0010041336836644184
24	7.809928650723256E-4
25	0.0021198377766248836
26	0.005690090874098372
27	0.005690090874098372
28	0.0039049643253616277
29	0.0021198377766248836
30	0.003793393916065581
31	0.008144639878611394
32	0.002008267367328837
33	0.004462816371841861
34	0.0016735561394406976
35	0.0014504153208486047
36	0.0078099286507232555
37	0.003793393916065581
38	0.007252076604243023
39	0.0041281051439537204
40	0.0018966969580327905
41	0.0032355418695853484
42	0.0016735561394406976
43	0.0022314081859209304
44	0.0011157040929604652
45	0.0013388449115525579
46	4.4628163718418603E-4
47	0.002008267367328837
48	0.0015619857301446512
49	1.1157040929604651E-4
50	7.809928650723256E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	896295.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.62390557505559
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.053601281221894	20.570547343657868
2	15.175785786250259	9.901868050357985
3	7.117369712780637	6.96589192357548
4	4.0428165594121195	5.275698627661283
5	2.5129872114833716	4.099172874937788
6	1.6639584718330016	3.257089443953618
7	1.1807191909028596	2.6963769976269645
8	0.8664293058163204	2.2613046288330074
9	0.6500461462129594	1.9086339684132332
>10	3.2218087146691525	18.81849520704271
>50	0.29070225862958615	6.6941140095232
>100	0.2035257499191099	12.48204932511023
>500	0.017847114664041624	4.075698085108187
>1k	0.002402496204774834	0.9930595141984756
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG	1746	0.1948019346308972	No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTAT	1566	0.1747192609576088	No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGA	1206	0.13455391361103208	No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT	1155	0.1288638227369337	No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	1106	0.12339687268142743	No Hit
GCCTAAAGGAAAGATCCAAAAAGATAAAAGGAACTCGGCAAACAAGAACC	1066	0.11893405630958556	No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT	1024	0.11424809911915162	No Hit
GATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGCAATC	994	0.11090098684027022	No Hit
ACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATC	977	0.10900428988223744	No Hit
CCTCTAGCATTACAAGTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTT	968	0.10800015619857302	No Hit
CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTA	963	0.10744230415209279	No Hit
ATCGTAAATAGATAGAAACCGACCTGGATTGCTCCGGTCTGAACTCAGAT	925	0.10320262859884301	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	2.2314081859209302E-4	0.0
16	0.0	0.0	0.0	2.2314081859209302E-4	0.0
17	0.0	0.0	0.0	2.2314081859209302E-4	0.0
18	0.0	0.0	0.0	3.347112278881395E-4	0.0
19	0.0	0.0	0.0	3.347112278881395E-4	0.0
20	0.0	0.0	0.0	3.347112278881395E-4	0.0
21	0.0	0.0	0.0	3.347112278881395E-4	0.0
22	0.0	0.0	0.0	3.347112278881395E-4	0.0
23	0.0	0.0	0.0	7.809928650723255E-4	0.0
24	0.0	0.0	0.0	0.001115704092960465	0.0
25	0.0	0.0	0.0	0.001115704092960465	0.0
26	0.0	0.0	0.0	0.001338844911552558	0.0
27	0.0	0.0	0.0	0.001561985730144651	0.0
28	0.0	0.0	0.0	0.0027892602324011626	0.0
29	0.0	0.0	0.0	0.006359513329874651	0.0
30	0.0	0.0	0.0	0.014838864436374185	0.0
31	0.0	0.0	0.0	0.04072319939305697	0.0
32	0.0	0.0	0.0	0.06961993540073302	0.0
33	0.0	0.0	0.0	0.09818196018052092	0.0
34	0.0	0.0	0.0	0.13500019524821627	0.0
35	0.0	0.0	0.0	0.1732688456367602	0.0
36	0.0	0.0	0.0	0.24110365448875648	0.0
37	0.0	0.0	0.0	0.34151702285519836	0.0
38	0.0	0.0	0.0	0.47651721810341463	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGTGCG	20	7.853541E-4	44.00229	26
ATCCGGT	30	0.0057427716	29.334858	23
AGAACGT	30	0.005745904	29.331585	5
GCGATAT	30	0.005745904	29.331585	13
CTAGGTA	60	9.745372E-6	25.668001	23
GTATCAA	1390	0.0	25.63876	1
GGTATCA	535	0.0	24.671427	1
AATCGGT	50	0.0025783076	22.00237	30
CTGTAGG	1350	0.0	21.346876	1
TAGGACG	1365	0.0	21.273457	4
TAGGACC	435	0.0	21.240112	4
TTAGGAC	790	0.0	20.88483	3
GGACGTG	1340	0.0	20.849503	6
TATGCAC	65	4.932776E-4	20.306482	3
ATATCGT	65	4.932776E-4	20.306482	11
CTCTAGG	195	0.0	20.306482	1
TGTAGGA	1440	0.0	20.012697	2
ACCGGTA	55	0.004481374	20.00104	34
GACCGTT	55	0.004484269	19.998808	7
GACGTGA	665	0.0	19.848442	7
>>END_MODULE