FastQCFastQC Report
Thu 2 Feb 2017
SRR4064099_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064099_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2477050
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT63300.2555459114672695No Hit
TATCAACGCAGAGTACTTTTTTTTT41120.16600391594840636No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAA602.568367E-514.25065110
TAGATCG400.00528645914.2474855
GTATCAA37150.013.1677891
GGTATCA12500.012.4623031
GCGTAAC907.459974E-611.611876511
GTCCTAA3550.011.5054761
TCGCGTA752.0742696E-411.4002919
CGCGATT855.3220087E-511.17720713
AGTTGCG3100.011.03410118
GTATTAG2500.011.01851
TTAGAAC2500.011.0167213
ACCGTCG2400.010.6879888
AAGACGG3600.010.5536925
CAAGACG4000.010.4477344
ACTGGTA2550.010.43184958
GTTGCGC3300.010.36536719
TGGTACG1659.295036E-1010.36411110
CGATTTA2700.010.20540818
CGACCCG2450.010.0798535
CGGACCA3150.09.9526359
Produced by FastQC (version 0.11.5)