##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064099_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2477050
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.25567953816031	32.0	32.0	32.0	32.0	32.0
2	30.867351486647422	32.0	32.0	32.0	32.0	32.0
3	30.893851153589956	32.0	32.0	32.0	32.0	32.0
4	30.912007831896812	32.0	32.0	32.0	32.0	32.0
5	30.834470842332614	32.0	32.0	32.0	32.0	32.0
6	34.492819280999576	36.0	36.0	36.0	32.0	36.0
7	34.43882844512626	36.0	36.0	36.0	32.0	36.0
8	34.39995720716175	36.0	36.0	36.0	32.0	36.0
9	34.527729759189356	36.0	36.0	36.0	32.0	36.0
10	34.247735411073656	36.0	36.0	36.0	32.0	36.0
11	34.50763044750813	36.0	36.0	36.0	32.0	36.0
12	34.3530606972003	36.0	36.0	36.0	32.0	36.0
13	34.41352334430068	36.0	36.0	36.0	32.0	36.0
14	34.31451726852506	36.0	36.0	36.0	32.0	36.0
15	34.26548030923881	36.0	36.0	36.0	32.0	36.0
16	34.25875052986415	36.0	36.0	36.0	32.0	36.0
17	34.20423689469328	36.0	36.0	36.0	32.0	36.0
18	34.19130255747764	36.0	36.0	36.0	32.0	36.0
19	34.20310772895178	36.0	36.0	36.0	32.0	36.0
20	34.18361034294826	36.0	36.0	36.0	32.0	36.0
21	34.16901637027916	36.0	36.0	36.0	32.0	36.0
22	34.157801820714155	36.0	36.0	36.0	32.0	36.0
23	34.098537373084916	36.0	36.0	36.0	32.0	36.0
24	34.0761708483882	36.0	36.0	36.0	32.0	36.0
25	33.68713994469228	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	10.0
4	24.0
5	29.0
6	100.0
7	38.0
8	154.0
9	165.0
10	189.0
11	73.0
12	131.0
13	146.0
14	268.0
15	452.0
16	767.0
17	977.0
18	1308.0
19	1741.0
20	2520.0
21	3760.0
22	5502.0
23	8256.0
24	12735.0
25	18463.0
26	26317.0
27	35543.0
28	48338.0
29	65130.0
30	85714.0
31	115590.0
32	163608.0
33	230778.0
34	501675.0
35	1146549.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.50543614563321	18.651708263482533	11.840341567886092	25.002514022998163
2	16.376215886247994	20.2064922360518	38.63200609472255	24.785285782977653
3	18.060960839725475	25.392208316511912	29.233225676221235	27.313605167541382
4	12.333041039081841	15.894065134786405	37.55278656544565	34.2201072606861
5	14.157462327717669	37.033116240535755	34.083972963730524	14.725448468016058
6	32.104982862338694	37.400841196927054	17.308089081115302	13.186086859618953
7	28.859911098763718	31.17216699993862	22.02776871464706	17.9401531866506
8	27.110313101611727	33.79578306903307	19.91837364371703	19.17553018563817
9	27.227934919012277	14.167591257857342	19.39460448028551	39.209869342844875
10	15.783138272819738	27.054921511268592	32.00551614490302	25.156424071008654
11	35.969828160760294	22.360872905530023	22.95274925818541	18.71654967552428
12	23.688973057172312	24.319844683663344	30.235301341485883	21.755880917678464
13	28.493532735439413	21.180851936220517	25.877905057914326	24.447710270425745
14	22.920248590930246	20.103401804643468	26.363743601013496	30.61260600341279
15	25.027115044133396	27.63395639698929	22.61081620625288	24.728112352624436
16	24.864893688359892	27.26154109663747	24.069081357873976	23.804483857128663
17	22.819324677674953	26.732306509033023	26.184585193710113	24.263783619581915
18	23.3763542081203	26.660188957863244	26.83476388347397	23.128692950542494
19	25.00877693022775	25.3551273230585	26.26518085728287	23.37091488943088
20	25.49661449253346	25.211010909014515	25.561338914771024	23.731035683681
21	26.045011920195893	24.971123673900934	25.35117208189524	23.632692324007934
22	24.890996417183942	25.233198176432275	26.13916420351429	23.736641202869485
23	23.96487956086118	25.486439334374666	26.214310475930354	24.3343706288338
24	24.294024295842362	25.55341966324547	25.824796497675294	24.327759543236873
25	24.426751219220517	25.481032035673035	25.443700919581747	24.648515825524704
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	137.0
1	137.0
2	441.0
3	745.0
4	745.0
5	745.0
6	1897.5
7	3050.0
8	3050.0
9	3050.0
10	3761.0
11	4472.0
12	4472.0
13	4472.0
14	6352.5
15	8233.0
16	8233.0
17	8233.0
18	14310.0
19	20387.0
20	20387.0
21	20387.0
22	32088.5
23	43790.0
24	43790.0
25	43790.0
26	64531.5
27	85273.0
28	85273.0
29	85273.0
30	107722.5
31	130172.0
32	130172.0
33	130172.0
34	156766.0
35	183360.0
36	183360.0
37	183360.0
38	211073.5
39	238787.0
40	238787.0
41	238787.0
42	262202.0
43	285617.0
44	285617.0
45	285617.0
46	307185.5
47	328754.0
48	328754.0
49	328754.0
50	326598.5
51	324443.0
52	324443.0
53	324443.0
54	298397.0
55	272351.0
56	272351.0
57	272351.0
58	241012.0
59	209673.0
60	209673.0
61	209673.0
62	178693.0
63	147713.0
64	147713.0
65	147713.0
66	119238.0
67	90763.0
68	90763.0
69	90763.0
70	69236.0
71	47709.0
72	47709.0
73	47709.0
74	37097.0
75	26485.0
76	26485.0
77	26485.0
78	20699.0
79	14913.0
80	14913.0
81	14913.0
82	10053.5
83	5194.0
84	5194.0
85	5194.0
86	3785.0
87	2376.0
88	2376.0
89	2376.0
90	1691.0
91	1006.0
92	1006.0
93	1006.0
94	724.0
95	442.0
96	442.0
97	442.0
98	823.5
99	1205.0
100	1205.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03790799539775136
2	0.006176702125512202
3	0.0020185301063765366
4	0.0037948365999878886
5	0.009244867887204538
6	0.013766375325487981
7	0.023980137663753252
8	0.03588946529137482
9	0.04767768111261379
10	0.056841807795563276
11	0.055065501301951925
12	0.06402777497426373
13	0.06390666316788114
14	0.06693445832744595
15	0.0600310853636382
16	0.06426999858702892
17	0.06140368583597424
18	0.07650229103167074
19	0.07311116045295815
20	0.07835933872953715
21	0.07424153731252901
22	0.07678488524656346
23	0.0817100987061222
24	0.07646192042954321
25	0.07682525584869097
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2477050.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.07681680263076
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.84704058304662	46.715685411960365
2	11.004477458898176	12.562010878604216
3	2.9505093819300723	5.052170504005982
4	1.2715621800041292	2.9030688640499793
5	0.7051129723520148	2.0122801974047198
6	0.4470125698904381	1.5308432736065827
7	0.30489474226410507	1.2181694943805554
8	0.21322643664811194	0.973622900963363
9	0.16412426607574435	0.8430921600904338
>10	0.9286623626997323	9.745799850256349
>50	0.08125475669295443	3.220835766632895
>100	0.07292574886132903	8.660877802771006
>500	0.007200392281346822	2.780472860850041
>1k	0.0019248573425374267	1.5234982787427838
>5k	7.129101268657136E-5	0.25757175568071955
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	6330	0.2555459114672695	No Hit
TATCAACGCAGAGTACTTTTTTTTT	4112	0.16600391594840636	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	4.037060212753073E-5	0.0
3	0.0	0.0	0.0	4.037060212753073E-5	0.0
4	0.0	0.0	0.0	4.037060212753073E-5	0.0
5	0.0	0.0	0.0	4.037060212753073E-5	0.0
6	0.0	0.0	0.0	4.037060212753073E-5	0.0
7	0.0	0.0	0.0	1.211118063825922E-4	0.0
8	0.0	0.0	0.0	1.211118063825922E-4	0.0
9	0.0	0.0	0.0	1.211118063825922E-4	0.0
10	0.0	0.0	0.0	1.211118063825922E-4	4.037060212753073E-5
11	0.0	0.0	0.0	1.211118063825922E-4	8.074120425506146E-5
12	0.0	0.0	0.0	1.211118063825922E-4	1.211118063825922E-4
13	0.0	0.0	0.0	1.211118063825922E-4	1.211118063825922E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGTAA	60	2.568367E-5	14.250651	10
TAGATCG	40	0.005286459	14.247485	5
GTATCAA	3715	0.0	13.167789	1
GGTATCA	1250	0.0	12.462303	1
GCGTAAC	90	7.459974E-6	11.6118765	11
GTCCTAA	355	0.0	11.505476	1
TCGCGTA	75	2.0742696E-4	11.400291	9
CGCGATT	85	5.3220087E-5	11.177207	13
AGTTGCG	310	0.0	11.034101	18
GTATTAG	250	0.0	11.0185	1
TTAGAAC	250	0.0	11.016721	3
ACCGTCG	240	0.0	10.687988	8
AAGACGG	360	0.0	10.553692	5
CAAGACG	400	0.0	10.447734	4
ACTGGTA	255	0.0	10.4318495	8
GTTGCGC	330	0.0	10.365367	19
TGGTACG	165	9.295036E-10	10.364111	10
CGATTTA	270	0.0	10.205408	18
CGACCCG	245	0.0	10.079853	5
CGGACCA	315	0.0	9.952635	9
>>END_MODULE