FastQCFastQC Report
Thu 2 Feb 2017
SRR4064099_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064099_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2477050
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA39350.026.9478911
GGTATCA16150.026.2910081
ATCAACG60450.017.3223
TTTACGG1154.2798492E-617.2159943
AACGCAG60700.017.177836
TCAACGC61600.016.9625644
CAACGCA62000.016.817655
TACCGTC3850.016.5700847
TATCAAC64400.016.5669782
TAGGACG4650.016.084394
TCTATAC2600.016.0756193
CTAACGT1105.6289733E-515.9987013
GTATAGA2850.015.438591
ACGCAGA70650.014.7900237
CGCAGAG71000.014.7171148
CCCTTAT6300.014.666661
CGATTTA5550.014.665771518
GTCCTAA5300.014.52829551
CGCAATA3950.014.48217536
AGAGTAC72950.014.4446311
Produced by FastQC (version 0.11.5)