##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064099_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2477050
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.362708059990716	32.0	32.0	32.0	32.0	32.0
2	31.428703498112675	32.0	32.0	32.0	32.0	32.0
3	31.502787186370885	32.0	32.0	32.0	32.0	32.0
4	31.576902767404775	32.0	32.0	32.0	32.0	32.0
5	31.530292081306392	32.0	32.0	32.0	32.0	32.0
6	35.12578712581498	36.0	36.0	36.0	36.0	36.0
7	35.145470620294304	36.0	36.0	36.0	36.0	36.0
8	35.08719283018106	36.0	36.0	36.0	36.0	36.0
9	35.201396822833615	36.0	36.0	36.0	36.0	36.0
10	35.06245412890333	36.0	36.0	36.0	36.0	36.0
11	35.199567227145195	36.0	36.0	36.0	36.0	36.0
12	35.11987888819362	36.0	36.0	36.0	36.0	36.0
13	35.148260228901314	36.0	36.0	36.0	36.0	36.0
14	35.09928140328213	36.0	36.0	36.0	36.0	36.0
15	35.073109545628874	36.0	36.0	36.0	36.0	36.0
16	35.08233180597889	36.0	36.0	36.0	36.0	36.0
17	35.05591005429846	36.0	36.0	36.0	36.0	36.0
18	35.048662723804526	36.0	36.0	36.0	36.0	36.0
19	35.05351930724047	36.0	36.0	36.0	36.0	36.0
20	35.042235320239804	36.0	36.0	36.0	36.0	36.0
21	35.037723098040004	36.0	36.0	36.0	36.0	36.0
22	35.02735350517753	36.0	36.0	36.0	36.0	36.0
23	34.97999031105549	36.0	36.0	36.0	36.0	36.0
24	34.954979915625444	36.0	36.0	36.0	36.0	36.0
25	34.92154296441331	36.0	36.0	36.0	36.0	36.0
26	34.86863729032518	36.0	36.0	36.0	32.0	36.0
27	34.86858036777618	36.0	36.0	36.0	32.0	36.0
28	34.841289033325936	36.0	36.0	36.0	32.0	36.0
29	34.81703114591954	36.0	36.0	36.0	32.0	36.0
30	34.80202256716659	36.0	36.0	36.0	32.0	36.0
31	34.781663268807655	36.0	36.0	36.0	32.0	36.0
32	34.75605458105407	36.0	36.0	36.0	32.0	36.0
33	34.74565148059183	36.0	36.0	36.0	32.0	36.0
34	34.727802426273186	36.0	36.0	36.0	32.0	36.0
35	34.69855432873781	36.0	36.0	36.0	32.0	36.0
36	34.667772552027614	36.0	36.0	36.0	32.0	36.0
37	34.65315637552734	36.0	36.0	36.0	32.0	36.0
38	34.630751498758606	36.0	36.0	36.0	32.0	36.0
39	34.61154478109041	36.0	36.0	36.0	32.0	36.0
40	34.59654992834218	36.0	36.0	36.0	32.0	36.0
41	34.57484386669627	36.0	36.0	36.0	32.0	36.0
42	34.5312807573525	36.0	36.0	36.0	32.0	36.0
43	34.518188167376515	36.0	36.0	36.0	32.0	36.0
44	34.45628348236814	36.0	36.0	36.0	32.0	36.0
45	34.437165983730644	36.0	36.0	36.0	32.0	36.0
46	34.4012543146081	36.0	36.0	36.0	32.0	36.0
47	34.373065137966535	36.0	36.0	36.0	32.0	36.0
48	34.32888597323429	36.0	36.0	36.0	32.0	36.0
49	34.300208716012996	36.0	36.0	36.0	32.0	36.0
50	33.74073837831291	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	7.0
18	0.0
19	9.0
20	26.0
21	81.0
22	219.0
23	620.0
24	1727.0
25	3832.0
26	8120.0
27	15291.0
28	25436.0
29	39681.0
30	58421.0
31	84658.0
32	123848.0
33	200646.0
34	420945.0
35	1493483.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.77325723643252	19.403902227110166	12.369662659144185	25.453177877313127
2	15.757166422154201	21.03136891723098	38.150605683077934	25.06085897753688
3	17.360431256487075	25.956453688089944	29.539398015616214	27.14371703980677
4	12.028663127510548	16.49013948043035	37.660765830322354	33.82043156173674
5	13.806786298217638	37.5027552935952	33.83403645465372	14.856421953533436
6	32.14858977298785	37.69010531891187	17.0773033061935	13.084001601906786
7	28.827390033374417	31.230130070200524	21.992275479633612	17.950204416791447
8	27.09207609538608	34.01022749247189	19.77292334548085	19.124773066661177
9	27.214042363017803	13.953062128308261	19.396419647972017	39.43647586070191
10	15.683159603071434	27.2904538518058	32.004949495765075	25.021437049357697
11	36.17629841949093	22.276538624573586	22.9260208716013	18.62114208433419
12	23.611542292092157	24.444469563960634	30.208994823271418	21.734993320675787
13	28.62854764433711	21.133849354327822	25.932981864175296	24.304621137159767
14	22.84878843590309	20.054088620074072	26.405524968046617	30.691597975976226
15	25.071126038125254	27.674619621541247	22.51066941037352	24.743584929959976
16	24.912132816909256	27.277582786509996	24.045007052789746	23.765277343791002
17	22.950646938899094	26.762842897801818	26.11428917462304	24.172220988676045
18	23.522230529615136	26.685718945646503	26.794195016317836	22.99785550842052
19	25.034759116496737	25.315698363535947	26.26251085970074	23.387031660266576
20	25.620288466080186	25.157153739978433	25.60442271055735	23.618135083384033
21	26.184856987141963	24.90393815223754	25.288548878706525	23.62265598191397
22	24.861205757821406	25.270806217723678	26.133203716682115	23.7347843077728
23	24.020295338515364	25.43328238505754	26.191652718465964	24.35476955796113
24	24.313514770463758	25.49020241110552	25.87324053970912	24.3230422787216
25	24.559518806365908	25.3538645631603	25.490117710608	24.596498919865788
26	24.19290372174295	25.916991534593187	25.923531957637437	23.966572786026425
27	24.223494154177587	25.847946038935003	25.859533117444933	24.069026689442477
28	23.84111227383421	25.874141684269002	26.276048227170257	24.008697814726535
29	24.1145640725854	25.204079820038338	26.062345476654556	24.619010630721704
30	24.183622674508033	25.68458212098836	26.054068937228823	24.07772626727478
31	24.89454321709401	25.356784682442658	25.325938977182254	24.42273312328108
32	23.979736434772008	25.746096623358167	25.700799125378794	24.573367816491036
33	23.927446902742403	25.439730116108052	26.103700689203553	24.529122291945995
34	24.304012718873096	24.941732752672742	26.649842205067603	24.104412323386555
35	24.65439462437393	25.138577238693593	25.969798438245622	24.237229698686857
36	24.694247508922658	25.642068119054922	25.357188998885675	24.306495373136737
37	24.65538068000142	25.559441180555332	25.49872100046186	24.286457138981387
38	24.19848049423907	25.751173462507744	25.937457859917686	24.1128881833355
39	24.685799552832105	24.69835545891231	25.894154115470734	24.72169087278485
40	24.839694354471785	25.279018420363126	25.863155519185206	24.018131705979886
41	24.52399119885343	25.556389915016453	26.12894890894043	23.790669977189687
42	24.444256044117974	26.110347416930598	25.903120514978834	23.542276023972597
43	24.139946822726543	25.453714536709356	25.910598232214966	24.49574040834914
44	24.097742114680294	25.024464999709327	26.17989122360525	24.69790166200513
45	24.695539695907076	25.311969369472664	25.494286247187127	24.498204687433134
46	24.739828293536824	25.422302139104357	25.313138620712483	24.52473094664633
47	23.94545140380398	25.633875713367548	26.032990120243007	24.387682762585467
48	24.382175520529287	25.950969681848758	25.50018207523785	24.16667272238411
49	24.214817945795723	25.507249185216047	25.842488424678358	24.435444444309876
50	24.26054111926699	25.91523503702442	25.562996414231005	24.26122742947759
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	28.0
1	37.5
2	47.0
3	121.0
4	195.0
5	269.0
6	343.0
7	340.5
8	338.0
9	486.5
10	635.0
11	918.5
12	1202.0
13	2430.0
14	3658.0
15	4868.0
16	6078.0
17	6295.0
18	6512.0
19	6508.5
20	6505.0
21	7681.5
22	8858.0
23	10736.5
24	12615.0
25	15760.5
26	18906.0
27	23528.5
28	28151.0
29	34212.5
30	40274.0
31	45873.5
32	51473.0
33	59162.0
34	66851.0
35	76677.0
36	86503.0
37	99700.5
38	112898.0
39	121946.5
40	130995.0
41	140682.0
42	150369.0
43	151150.5
44	151932.0
45	160301.5
46	168671.0
47	173895.5
48	179120.0
49	183954.0
50	188788.0
51	186631.0
52	184474.0
53	177364.5
54	170255.0
55	164951.5
56	159648.0
57	150460.0
58	141272.0
59	129190.5
60	117109.0
61	102113.0
62	87117.0
63	75464.0
64	63811.0
65	52464.0
66	41117.0
67	34412.0
68	27707.0
69	24403.5
70	21100.0
71	17508.0
72	13916.0
73	12461.5
74	11007.0
75	8796.0
76	6585.0
77	5527.5
78	4470.0
79	3381.0
80	2292.0
81	1836.5
82	1381.0
83	1138.0
84	895.0
85	654.0
86	413.0
87	301.0
88	189.0
89	154.0
90	119.0
91	97.0
92	75.0
93	66.0
94	57.0
95	55.5
96	54.0
97	41.0
98	28.0
99	21.0
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008397085242526392
2	4.8444722553036884E-4
3	3.6333541914777655E-4
4	0.0
5	0.0
6	8.074120425506146E-5
7	1.2111180638259221E-4
8	4.037060212753073E-5
9	0.0012514886659534526
10	0.001211118063825922
11	0.0
12	1.2111180638259221E-4
13	7.67041440423084E-4
14	1.6148240851012293E-4
15	0.00440039563190085
16	6.459296340404917E-4
17	0.0
18	2.4222361276518442E-4
19	8.074120425506146E-5
20	6.863002361680225E-4
21	0.0
22	8.074120425506146E-5
23	0.0011303768595708604
24	4.8444722553036884E-4
25	0.0017359358914838215
26	0.005894107910619487
27	0.006176702125512202
28	0.004279283825518258
29	0.003148906965947397
30	0.004077430814880604
31	0.00831634403827133
32	0.0037140953957328273
33	0.005369290082961587
34	0.004077430814880604
35	0.0037140953957328273
36	0.008477826446781453
37	0.004602248642538503
38	0.0077511556084859014
39	0.005167437072323934
40	0.0025433479340344362
41	0.004037060212753073
42	0.0022203831170141903
43	0.002099271310631598
44	0.0016955652893562907
45	0.0017763064936113524
46	0.0012918592680809834
47	0.002664459740417028
48	0.002099271310631598
49	5.248178276578995E-4
50	0.0014533416765911062
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2477050.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.387077786388666
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.03008024369582	50.96973994529848
2	10.556754925980108	12.960966716259653
3	2.7214514754772394	5.011858602510106
4	1.124584381256987	2.761397955583217
5	0.6324567562599163	1.9412336046527268
6	0.3895750372627747	1.4348923869651131
7	0.2653003021208945	1.1400207198133439
8	0.20050157629099224	0.9846564688054946
9	0.14624441013275621	0.807976528257963
>10	0.803403175219229	8.97000643149509
>50	0.0652783246575336	2.7674486235258935
>100	0.05781369575244834	7.18097081199653
>500	0.00549618948633257	2.2915625282843486
>1k	0.0010595064070020317	0.7772686765520288
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.6148240851012293E-4
2	0.0	0.0	0.0	0.0	1.6148240851012293E-4
3	0.0	0.0	0.0	0.0	1.6148240851012293E-4
4	0.0	0.0	0.0	4.037060212753073E-5	1.6148240851012293E-4
5	0.0	0.0	0.0	4.037060212753073E-5	1.6148240851012293E-4
6	0.0	0.0	0.0	4.037060212753073E-5	1.6148240851012293E-4
7	0.0	0.0	0.0	4.037060212753073E-5	1.6148240851012293E-4
8	0.0	0.0	0.0	4.037060212753073E-5	1.6148240851012293E-4
9	0.0	0.0	0.0	4.037060212753073E-5	1.6148240851012293E-4
10	0.0	0.0	0.0	8.074120425506146E-5	1.6148240851012293E-4
11	0.0	0.0	0.0	8.074120425506146E-5	1.6148240851012293E-4
12	0.0	0.0	0.0	8.074120425506146E-5	1.6148240851012293E-4
13	0.0	0.0	0.0	8.074120425506146E-5	2.825942148927151E-4
14	0.0	0.0	0.0	8.074120425506146E-5	2.825942148927151E-4
15	0.0	0.0	0.0	8.074120425506146E-5	3.2296481702024586E-4
16	0.0	0.0	0.0	8.074120425506146E-5	3.2296481702024586E-4
17	0.0	0.0	0.0	8.074120425506146E-5	3.2296481702024586E-4
18	0.0	0.0	0.0	8.074120425506146E-5	3.633354191477766E-4
19	0.0	0.0	0.0	8.074120425506146E-5	3.633354191477766E-4
20	0.0	0.0	0.0	8.074120425506146E-5	3.633354191477766E-4
21	0.0	0.0	0.0	1.211118063825922E-4	3.633354191477766E-4
22	0.0	0.0	0.0	2.0185301063765365E-4	3.633354191477766E-4
23	0.0	0.0	0.0	2.422236127651844E-4	3.633354191477766E-4
24	0.0	4.037060212753073E-5	0.0	5.248178276578995E-4	3.633354191477766E-4
25	0.0	4.037060212753073E-5	0.0	6.05559031912961E-4	3.633354191477766E-4
26	0.0	4.037060212753073E-5	0.0	7.266708382955532E-4	4.4407662340283804E-4
27	0.0	4.037060212753073E-5	0.0	8.881532468056761E-4	4.844472255303688E-4
28	0.0	4.037060212753073E-5	0.0	0.0017359358914838215	4.844472255303688E-4
29	0.0	4.037060212753073E-5	0.0	0.004158172019135665	4.844472255303688E-4
30	0.0	4.037060212753073E-5	0.0	0.009648573908479846	4.844472255303688E-4
31	0.0	4.037060212753073E-5	0.0	0.023051613814820047	4.844472255303688E-4
32	0.0	4.037060212753073E-5	0.0	0.03871540744030197	4.844472255303688E-4
33	0.0	4.037060212753073E-5	0.0	0.055832542742375	4.844472255303688E-4
34	0.0	4.037060212753073E-5	0.0	0.07508931995720716	5.248178276578995E-4
35	0.0	4.037060212753073E-5	0.0	0.09935205183585313	5.651884297854302E-4
36	0.0	4.037060212753073E-5	0.0	0.13140630992511254	5.651884297854302E-4
37	0.0	4.037060212753073E-5	0.0	0.17795361417815547	5.651884297854302E-4
38	0.0	4.037060212753073E-5	0.0	0.24581659635453462	5.651884297854302E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3935	0.0	26.947891	1
GGTATCA	1615	0.0	26.291008	1
ATCAACG	6045	0.0	17.322	3
TTTACGG	115	4.2798492E-6	17.215994	3
AACGCAG	6070	0.0	17.17783	6
TCAACGC	6160	0.0	16.962564	4
CAACGCA	6200	0.0	16.81765	5
TACCGTC	385	0.0	16.570084	7
TATCAAC	6440	0.0	16.566978	2
TAGGACG	465	0.0	16.08439	4
TCTATAC	260	0.0	16.075619	3
CTAACGT	110	5.6289733E-5	15.998701	3
GTATAGA	285	0.0	15.43859	1
ACGCAGA	7065	0.0	14.790023	7
CGCAGAG	7100	0.0	14.717114	8
CCCTTAT	630	0.0	14.66666	1
CGATTTA	555	0.0	14.6657715	18
GTCCTAA	530	0.0	14.5282955	1
CGCAATA	395	0.0	14.482175	36
AGAGTAC	7295	0.0	14.44463	11
>>END_MODULE