FastQCFastQC Report
Thu 2 Feb 2017
SRR4064098_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064098_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1570886
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT33850.21548349148187712No Hit
TATCAACGCAGAGTACTTTTTTTTT22540.14348590540624845No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAACGA1051.369699E-913.57199712
CGAACGT707.276254E-613.5689724
CGATAAC1155.326001E-912.39103410
ACTCTAA2250.012.24417810
AACCGCG1103.8164217E-812.09026057
ACTCCGT550.00307116812.089496
CGTAAAT550.003075566412.0871793
CGCGCTA802.8586426E-511.87814616
CGAACGA1302.6029738E-911.69540616
ACGAACG1302.6029738E-911.69540615
GATAACG1302.6138878E-911.69242611
GTATAAT1302.6266207E-911.68907451
GTATTAG2200.011.6558671
AACGAAC1157.0529495E-811.56754414
TAACGAA1157.068593E-811.565701513
CGCGGTC1157.0747774E-811.56496510
GTTCTAA1157.0998794E-811.5620181
GCGCCGA1751.8189894E-1211.40229419
ACGGTAT2000.011.4001149
CGTCGTA2250.011.39975210
Produced by FastQC (version 0.11.5)