FastQCFastQC Report
Thu 2 Feb 2017
SRR4064098_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064098_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1570886
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA25000.023.583881
TTAACGG1351.1277734E-1021.18710135
TACGCTA3850.021.1420789
TAGGACG2500.021.118554
TTAGACG956.8735426E-720.8406724
CGTCGTA4150.020.67393710
ACGCTAT4000.020.34924910
ATACGCT4150.019.6137338
ATATACG4150.019.613116
TACCGTC4150.019.613117
GTCGTAG4300.019.44114311
ACCGTCG4100.019.316368
ATACCGT4150.019.0830256
AACGCAG31750.018.430236
TAATACG600.007416005218.3320734
CGATTAA851.4315824E-418.1169841
CGCTATT4550.017.8894511
TAACGGC1509.591531E-917.60159336
TATACGC4650.017.5041757
TAAACGC4550.017.40816728