##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064098_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1570886
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.352502345809945	32.0	32.0	32.0	32.0	32.0
2	31.438942736774024	32.0	32.0	32.0	32.0	32.0
3	31.508080153492998	32.0	32.0	32.0	32.0	32.0
4	31.591253598287846	32.0	32.0	32.0	32.0	32.0
5	31.54256960721529	32.0	32.0	32.0	32.0	32.0
6	35.14246609874937	36.0	36.0	36.0	36.0	36.0
7	35.15814896816192	36.0	36.0	36.0	36.0	36.0
8	35.11036319631087	36.0	36.0	36.0	36.0	36.0
9	35.20430890592952	36.0	36.0	36.0	36.0	36.0
10	35.07256541849631	36.0	36.0	36.0	36.0	36.0
11	35.211659534810295	36.0	36.0	36.0	36.0	36.0
12	35.123847943135274	36.0	36.0	36.0	36.0	36.0
13	35.15839023328236	36.0	36.0	36.0	36.0	36.0
14	35.10748456603471	36.0	36.0	36.0	36.0	36.0
15	35.07984665978308	36.0	36.0	36.0	36.0	36.0
16	35.098215911275545	36.0	36.0	36.0	36.0	36.0
17	35.06583736821131	36.0	36.0	36.0	36.0	36.0
18	35.05555718237988	36.0	36.0	36.0	36.0	36.0
19	35.05837979331409	36.0	36.0	36.0	36.0	36.0
20	35.04599569924234	36.0	36.0	36.0	36.0	36.0
21	35.04898573161897	36.0	36.0	36.0	36.0	36.0
22	35.03177187905424	36.0	36.0	36.0	36.0	36.0
23	34.98364489848404	36.0	36.0	36.0	36.0	36.0
24	34.95308634744978	36.0	36.0	36.0	36.0	36.0
25	34.92527401733798	36.0	36.0	36.0	36.0	36.0
26	34.873815795671995	36.0	36.0	36.0	32.0	36.0
27	34.86589669778711	36.0	36.0	36.0	32.0	36.0
28	34.849302877484426	36.0	36.0	36.0	32.0	36.0
29	34.82453596250778	36.0	36.0	36.0	32.0	36.0
30	34.810687089960695	36.0	36.0	36.0	32.0	36.0
31	34.79920949069506	36.0	36.0	36.0	32.0	36.0
32	34.760821600039726	36.0	36.0	36.0	32.0	36.0
33	34.73424042228398	36.0	36.0	36.0	32.0	36.0
34	34.72933872986327	36.0	36.0	36.0	32.0	36.0
35	34.69199356286834	36.0	36.0	36.0	32.0	36.0
36	34.6739222324217	36.0	36.0	36.0	32.0	36.0
37	34.65644037823241	36.0	36.0	36.0	32.0	36.0
38	34.62369388994491	36.0	36.0	36.0	32.0	36.0
39	34.624671682095325	36.0	36.0	36.0	32.0	36.0
40	34.60407311542658	36.0	36.0	36.0	32.0	36.0
41	34.587594516724955	36.0	36.0	36.0	32.0	36.0
42	34.52372037181565	36.0	36.0	36.0	32.0	36.0
43	34.522833611095905	36.0	36.0	36.0	32.0	36.0
44	34.45927011890105	36.0	36.0	36.0	32.0	36.0
45	34.433685194215236	36.0	36.0	36.0	32.0	36.0
46	34.40762728804	36.0	36.0	36.0	32.0	36.0
47	34.38061132380071	36.0	36.0	36.0	32.0	36.0
48	34.33658012102724	36.0	36.0	36.0	32.0	36.0
49	34.3255424009126	36.0	36.0	36.0	32.0	36.0
50	33.73039100227515	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	3.0
18	2.0
19	7.0
20	10.0
21	35.0
22	157.0
23	458.0
24	1142.0
25	2564.0
26	5472.0
27	9969.0
28	16633.0
29	25581.0
30	36754.0
31	52330.0
32	76610.0
33	123699.0
34	264177.0
35	955283.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.58204356243518	18.15595919674399	11.767128842971891	26.494868397848936
2	15.92940240311928	20.682485875706213	37.100533142356966	26.287578578817538
3	17.84438159224028	24.6893940406689	29.19762859185387	28.268595775236953
4	12.117938539142878	16.261141801505648	36.6029107140811	35.018008945270374
5	14.044876585570181	37.228162960265735	33.75903789326533	14.967922560898753
6	33.75746873412516	36.27115212982261	16.96416408107038	13.007215054981852
7	29.91483786856589	30.834955560110664	21.17556589084122	18.074640680482222
8	27.91889417818989	33.01659063738553	19.627204010984883	19.437311173439703
9	27.657840777504966	13.896176070473004	18.374455954690106	40.071527197331925
10	15.396671402889606	26.964259341308523	32.22598658579439	25.413082670007487
11	36.94717503370709	21.013364432555896	23.015610298901386	19.023850234835628
12	24.527017908387887	23.76356242727025	29.225200587694573	22.48421907664729
13	29.463669020553485	19.57702608160919	25.41274714697586	25.54655775086146
14	23.543558913325235	19.6183168203381	25.564268271877488	31.273855994459172
15	25.047665792608125	27.395783001757668	22.42109759598888	25.13545360964533
16	25.70379834346467	25.849068254577563	23.800456944531977	24.646676457425794
17	24.073993911716062	25.84503267582753	25.29273289086541	24.788240521591
18	24.576877022355	25.186599349790782	26.249889393135945	23.986634234718267
19	25.57117448369901	24.987045527173837	25.445640231054323	23.996139758072832
20	25.721171750119836	24.46009595889186	25.132011909240738	24.686720381747566
21	26.694680581531692	24.301827121764404	24.56492705390461	24.43856524279929
22	25.79821298071659	24.475581901655374	24.990132944253045	24.736072173374993
23	24.45972682688341	24.245259957526773	25.493611191745728	25.801402023844084
24	24.861049132364744	24.90834749207291	25.33581517099662	24.894788204565724
25	24.958971362074386	24.445747345074718	25.48874688545212	25.10653440739878
26	24.71530647843281	25.140946211258893	25.68856107541832	24.45518623488998
27	25.242760476118022	24.936353560349048	24.850154731354408	24.97073123217852
28	24.601531042605	24.797606353349355	25.678799357025767	24.922063247019878
29	24.76510960066563	24.863910305257253	25.42042819155878	24.950551902518335
30	24.704979867267202	25.035888784555887	25.66982318208585	24.58930816609107
31	25.192596011522973	24.70245579411437	24.875618723241715	25.22932947112094
32	24.910158903356123	25.02035549381887	24.773479481216608	25.2960061216084
33	24.510408085571793	24.69012565587682	25.315030958786554	25.484435299764836
34	25.199145170192487	24.60774146809444	25.461361730896677	24.731751630816397
35	25.6089913962956	24.548727269254886	25.420174620504383	24.42210671394513
36	24.723272838625107	25.199217454358404	25.066989395653472	25.010520311363017
37	25.61628286719942	24.895453292497148	24.856938597390297	24.63132524291314
38	24.770702944207937	24.843915025601945	25.370660035561972	25.014721994628143
39	25.291743086559414	24.763673935283357	24.811674434386692	25.132908543770533
40	25.581103697559342	24.879189379996905	25.22830040099386	24.311406521449893
41	24.62876502860257	25.221442312956523	25.491043634034828	24.65874902440608
42	25.645032943947545	25.258299646688098	25.033898844574594	24.062768564789764
43	24.99420696539203	24.512302877674028	25.291943480353336	25.201546676580605
44	24.734875033659844	25.11765825536664	24.889630731642416	25.257835979331105
45	24.85071871458946	25.247826031600525	25.006429599073122	24.895025654736898
46	24.703300788672752	24.96264801539532	25.040757747154913	25.293293448777014
47	24.826892091561145	24.90302965099587	25.42720746402723	24.842870793415756
48	25.50662158695277	25.260003042944344	24.603159951287427	24.630215418815453
49	24.7823828457716	25.284267778910902	24.852343721154664	25.08100565416283
50	24.81446539679387	25.48836119694487	24.848459384823403	24.848714021437857
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	80.0
1	63.5
2	47.0
3	134.0
4	221.0
5	244.5
6	268.0
7	261.5
8	255.0
9	337.0
10	419.0
11	614.5
12	810.0
13	1467.5
14	2125.0
15	2808.5
16	3492.0
17	3676.5
18	3861.0
19	3960.0
20	4059.0
21	4648.0
22	5237.0
23	6043.5
24	6850.0
25	8259.5
26	9669.0
27	12231.0
28	14793.0
29	17282.5
30	19772.0
31	22539.0
32	25306.0
33	29286.0
34	33266.0
35	38591.0
36	43916.0
37	51299.0
38	58682.0
39	64311.5
40	69941.0
41	76535.5
42	83130.0
43	85599.0
44	88068.0
45	96845.0
46	105622.0
47	111087.5
48	116553.0
49	120145.0
50	123737.0
51	121302.5
52	118868.0
53	116926.0
54	114984.0
55	114219.0
56	113454.0
57	109494.5
58	105535.0
59	96054.0
60	86573.0
61	75572.5
62	64572.0
63	55735.0
64	46898.0
65	39622.5
66	32347.0
67	27402.5
68	22458.0
69	20007.0
70	17556.0
71	13670.0
72	9784.0
73	8342.0
74	6900.0
75	5276.5
76	3653.0
77	3298.0
78	2943.0
79	2364.0
80	1785.0
81	1448.5
82	1112.0
83	889.0
84	666.0
85	478.5
86	291.0
87	207.5
88	124.0
89	97.5
90	71.0
91	57.5
92	44.0
93	36.0
94	28.0
95	28.0
96	28.0
97	21.5
98	15.0
99	16.5
100	18.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00821192626326799
2	7.002417743871929E-4
3	3.182917156305423E-4
4	0.0
5	0.0
6	2.546333725044338E-4
7	0.0
8	0.0
9	9.548751468916268E-4
10	8.912168037655183E-4
11	0.0
12	1.273166862522169E-4
13	5.72925088134976E-4
14	1.273166862522169E-4
15	0.0033738921856837478
16	0.0010821918331438438
17	0.0
18	4.456084018827592E-4
19	0.0
20	3.182917156305423E-4
21	0.0
22	1.273166862522169E-4
23	8.912168037655183E-4
24	4.456084018827592E-4
25	0.0020370669800354704
26	0.0047107173913320255
27	0.006302175969484737
28	0.0038831589306926157
29	0.0029919421269270973
30	0.0038831589306926157
31	0.007066076086998037
32	0.003628525558188182
33	0.005601934195097543
34	0.0029919421269270973
35	0.003246575499431531
36	0.0073207094595024716
37	0.003628525558188182
38	0.006811442714493604
39	0.004392425675701484
40	0.00248267538191823
41	0.0030556004700532055
42	0.002291700352539904
43	0.0021643836662876875
44	0.0014641418919004944
45	0.0016551169212788198
46	0.0012095085193960604
47	0.0028646254406748803
48	0.00248267538191823
49	5.092667450088676E-4
50	0.0012731668625221692
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1570886.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.66072239410973
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.91114848337784	45.50073826500423
2	12.690558717322691	14.634935664513863
3	3.6612961736019334	6.33338946806032
4	1.5475909509795907	3.5694084881628183
5	0.8314653077795704	2.397144514610541
6	0.5097544616291733	1.7635686300695175
7	0.35097557655503736	1.4166253700796831
8	0.2416918526181585	1.1148901454986977
9	0.19769164109821788	1.0259138553300284
>10	0.9308521253867243	9.489228814365614
>50	0.06315197272591284	2.5328725361276563
>100	0.05548370415580205	6.535141682766661
>500	0.006448852008328217	2.4219320942937097
>1k	0.0018901807610617186	1.264210471116755
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	1.273166862522169E-4
5	0.0	0.0	0.0	0.0	1.273166862522169E-4
6	0.0	0.0	0.0	0.0	1.273166862522169E-4
7	0.0	0.0	0.0	0.0	1.273166862522169E-4
8	0.0	0.0	0.0	6.365834312610845E-5	1.273166862522169E-4
9	0.0	0.0	0.0	1.9097502937832534E-4	1.273166862522169E-4
10	0.0	0.0	0.0	1.9097502937832534E-4	1.273166862522169E-4
11	0.0	0.0	0.0	1.9097502937832534E-4	1.273166862522169E-4
12	0.0	0.0	0.0	1.9097502937832534E-4	2.546333725044338E-4
13	0.0	0.0	0.0	1.9097502937832534E-4	2.546333725044338E-4
14	0.0	0.0	0.0	1.9097502937832534E-4	2.546333725044338E-4
15	0.0	0.0	0.0	1.9097502937832534E-4	2.546333725044338E-4
16	0.0	0.0	0.0	1.9097502937832534E-4	2.546333725044338E-4
17	0.0	0.0	0.0	2.546333725044338E-4	2.546333725044338E-4
18	0.0	0.0	0.0	3.1829171563054224E-4	2.546333725044338E-4
19	0.0	0.0	0.0	3.819500587566507E-4	2.546333725044338E-4
20	0.0	0.0	0.0	3.819500587566507E-4	3.1829171563054224E-4
21	0.0	0.0	0.0	5.092667450088676E-4	3.819500587566507E-4
22	0.0	0.0	0.0	7.639001175133014E-4	6.365834312610845E-4
23	0.0	0.0	0.0	0.001273166862522169	7.002417743871929E-4
24	0.0	0.0	0.0	0.0022280420094137957	7.002417743871929E-4
25	0.0	0.0	0.0	0.002546333725044338	7.002417743871929E-4
26	0.0	0.0	0.0	0.003119258813179314	7.002417743871929E-4
27	0.0	0.0	0.0	0.00369218390131429	7.639001175133014E-4
28	0.0	0.0	0.0	0.0052836424794670015	7.639001175133014E-4
29	0.0	0.0	0.0	0.010058018213925135	7.639001175133014E-4
30	0.0	0.0	0.0	0.019861403055345835	7.639001175133014E-4
31	0.0	0.0	0.0	0.04023207285570054	7.639001175133014E-4
32	0.0	0.0	0.0	0.06467687661612619	7.639001175133014E-4
33	0.0	0.0	0.0	0.08632071327900306	7.639001175133014E-4
34	0.0	0.0	0.0	0.11031990863754594	8.912168037655183E-4
35	0.0	0.0	0.0	0.13845689629928587	8.912168037655183E-4
36	0.0	0.0	0.0	0.17569702702805934	8.912168037655183E-4
37	0.0	0.0	0.0	0.23407172767470077	8.912168037655183E-4
38	0.0	0.0	0.0	0.3165092820230112	8.912168037655183E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2500	0.0	23.58388	1
TTAACGG	135	1.1277734E-10	21.187101	35
TACGCTA	385	0.0	21.142078	9
TAGGACG	250	0.0	21.11855	4
TTAGACG	95	6.8735426E-7	20.840672	4
CGTCGTA	415	0.0	20.673937	10
ACGCTAT	400	0.0	20.349249	10
ATACGCT	415	0.0	19.613733	8
ATATACG	415	0.0	19.61311	6
TACCGTC	415	0.0	19.61311	7
GTCGTAG	430	0.0	19.441143	11
ACCGTCG	410	0.0	19.31636	8
ATACCGT	415	0.0	19.083025	6
AACGCAG	3175	0.0	18.43023	6
TAATACG	60	0.0074160052	18.332073	4
CGATTAA	85	1.4315824E-4	18.11698	41
CGCTATT	455	0.0	17.88945	11
TAACGGC	150	9.591531E-9	17.601593	36
TATACGC	465	0.0	17.504175	7
TAAACGC	455	0.0	17.408167	28
>>END_MODULE