FastQCFastQC Report
Thu 2 Feb 2017
SRR4064097_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064097_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences899945
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT33430.3714671452144298No Hit
TATCAACGCAGAGTACTTTTTTTTT25470.28301729550139176No Hit
GTACTTTTTTTTTTTTTTTTTTTTT18860.20956836251104236No Hit
GTACATGGGGTGGTATCAACGCAAA12920.14356432893121246No Hit
ACGCAGAGTACTTTTTTTTTTTTTT12680.14089749929162337No Hit
GGTATCAACGCAGAGTACTTTTTTT12640.14045302768502518No Hit
GAGTACTTTTTTTTTTTTTTTTTTT12460.1384529054553334No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG10070.11189572696109207No Hit
CTATTGGAGCTGGAATTACCGCGGC9570.1063398318786148No Hit
GTATCTGATCGTCTTCGAACCTCCG9560.10622871397696525No Hit
GATTAAGAGGGACGGCCGGGGGCAT9430.10478418125552118No Hit
GAATAGGACCGCGGTTCTATTTTGT9370.1041174738456239No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAT453.5448327E-516.8840941
CGCATCG1100.015.54709313
GTTTAGA508.728543E-515.1956851
CCGCATC1200.015.04325212
TTTGCGC1101.2732926E-1114.68418317
ATTTGCG1152.5465852E-1114.04652116
TTGCGCG1051.3624231E-913.57436918
CATCGCC1351.8189894E-1213.37326615
GCTCGTA500.001497513.3014029
TCGCGTA500.001497513.3014029
GCGTTAT1001.0089934E-813.2962251
TATAGGA500.001504419113.2932682
CGACCAT1403.6379788E-1212.89421710
CCGACCA1550.012.8723259
GCGATCG604.0854118E-412.66800114
GCATCGC1352.7284841E-1112.66800114
AGAACCG1051.9939762E-812.6644795
GTCTTAG905.415095E-712.6630711
CGTTATT905.42972E-712.6602552
CGTCACT1001.4357283E-712.35267515
Produced by FastQC (version 0.11.5)