##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064097_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	899945
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.182908955547283	32.0	32.0	32.0	32.0	32.0
2	30.762058792481763	32.0	32.0	32.0	32.0	32.0
3	30.788980437693414	32.0	32.0	32.0	32.0	32.0
4	30.802871286578625	32.0	32.0	32.0	32.0	32.0
5	30.724025357105155	32.0	32.0	32.0	32.0	32.0
6	34.35897749306902	36.0	36.0	36.0	32.0	36.0
7	34.2625527115546	36.0	36.0	36.0	32.0	36.0
8	34.218197778753144	36.0	36.0	36.0	32.0	36.0
9	34.35685514114751	36.0	36.0	36.0	32.0	36.0
10	34.036636683352874	36.0	36.0	36.0	32.0	36.0
11	34.37894315763741	36.0	36.0	36.0	32.0	36.0
12	34.149754707232105	36.0	36.0	36.0	32.0	36.0
13	34.24895076921367	36.0	36.0	36.0	32.0	36.0
14	34.14787236997817	36.0	36.0	36.0	32.0	36.0
15	34.06868197500958	36.0	36.0	36.0	32.0	36.0
16	34.07241887004206	36.0	36.0	36.0	32.0	36.0
17	34.000875609065	36.0	36.0	36.0	32.0	36.0
18	34.00273350038058	36.0	36.0	36.0	32.0	36.0
19	34.01129513470268	36.0	36.0	36.0	32.0	36.0
20	33.985372439426854	36.0	36.0	36.0	32.0	36.0
21	33.96444449383018	36.0	36.0	36.0	32.0	36.0
22	33.94846796193101	36.0	36.0	36.0	32.0	36.0
23	33.89272566656851	36.0	36.0	36.0	32.0	36.0
24	33.89305568673641	36.0	36.0	36.0	32.0	36.0
25	33.47432009733928	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	9.0
5	10.0
6	29.0
7	16.0
8	57.0
9	52.0
10	82.0
11	25.0
12	60.0
13	38.0
14	84.0
15	181.0
16	276.0
17	389.0
18	503.0
19	643.0
20	1069.0
21	1518.0
22	2341.0
23	3643.0
24	5429.0
25	7969.0
26	11176.0
27	14761.0
28	19787.0
29	26170.0
30	34549.0
31	45802.0
32	64428.0
33	89226.0
34	185988.0
35	383634.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.38978709780695	17.65658627252192	11.670379559966563	25.28324706970457
2	16.35916919565418	20.174375124319507	38.12505625631321	25.341399423713106
3	18.36718592869898	24.817458227352986	29.064672736020874	27.750683107927166
4	12.130465510114277	15.782806395263558	36.64797603331866	35.4387520613035
5	14.08422531204438	37.44476992283347	33.608113153870775	14.862891611251378
6	33.06191078951843	36.45089556103562	17.35455815395626	13.132635495489689
7	29.54248656578735	31.110246678262417	21.18832348806064	18.158943267889594
8	28.319722197211966	32.535006019134514	19.676067187546895	19.469204596106625
9	27.88455338422098	13.907201572432623	18.848179762489746	39.36006528085665
10	15.853379228761137	26.816805197924033	31.776802351695956	25.55301322161888
11	36.53244303027675	21.340702125365077	23.09551524288219	19.031339601475985
12	24.796527400976668	24.05290732323457	29.217330973199434	21.933234302589327
13	29.430221762648923	19.79341438872118	25.622063965932274	25.15429988269762
14	23.661088563962863	20.079168288208148	25.97631622838717	30.28342691944182
15	24.90262985558198	27.663905946500066	22.935102506885123	24.49836169103283
16	25.217318789819544	26.061576436839122	24.413200351357062	24.30790442198428
17	23.413639972158784	26.282041303733884	25.697531426995752	24.606787297111584
18	24.19162209347582	25.12248824046171	27.150021684255005	23.535867981807463
19	25.369288865957373	25.005003914172864	26.128215848841762	23.497491371028005
20	25.781725216594513	24.273486785260342	25.51795307480253	24.42683492334261
21	27.037915508807515	24.13384351415396	24.87020301593815	23.95803796110038
22	25.728786380938296	24.486221586644263	25.496041785049854	24.288950247367584
23	24.159861747923493	24.54864010978318	26.02802184542946	25.263476296863864
24	24.446638799835867	25.260181234480587	25.83430911721305	24.458870848470493
25	24.55897267098427	24.827859227101275	25.90173742082414	24.711430681090313
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	112.0
1	112.0
2	336.5
3	561.0
4	561.0
5	561.0
6	1505.5
7	2450.0
8	2450.0
9	2450.0
10	2401.5
11	2353.0
12	2353.0
13	2353.0
14	2444.0
15	2535.0
16	2535.0
17	2535.0
18	4556.0
19	6577.0
20	6577.0
21	6577.0
22	10801.5
23	15026.0
24	15026.0
25	15026.0
26	22549.0
27	30072.0
28	30072.0
29	30072.0
30	37015.5
31	43959.0
32	43959.0
33	43959.0
34	52826.0
35	61693.0
36	61693.0
37	61693.0
38	69333.5
39	76974.0
40	76974.0
41	76974.0
42	86581.5
43	96189.0
44	96189.0
45	96189.0
46	107054.5
47	117920.0
48	117920.0
49	117920.0
50	119056.0
51	120192.0
52	120192.0
53	120192.0
54	108885.0
55	97578.0
56	97578.0
57	97578.0
58	89077.0
59	80576.0
60	80576.0
61	80576.0
62	70894.5
63	61213.0
64	61213.0
65	61213.0
66	50796.5
67	40380.0
68	40380.0
69	40380.0
70	30383.5
71	20387.0
72	20387.0
73	20387.0
74	15657.5
75	10928.0
76	10928.0
77	10928.0
78	8848.0
79	6768.0
80	6768.0
81	6768.0
82	4687.5
83	2607.0
84	2607.0
85	2607.0
86	2018.5
87	1430.0
88	1430.0
89	1430.0
90	1032.0
91	634.0
92	634.0
93	634.0
94	464.0
95	294.0
96	294.0
97	294.0
98	415.5
99	537.0
100	537.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04189144892187856
2	0.005111423475879081
3	0.0015556506230936335
4	0.003222419147836812
5	0.008111606820416804
6	0.01311191239464634
7	0.0211124013134136
8	0.03489102111795721
9	0.049669702037346726
10	0.056559011939618535
11	0.052892121185183535
12	0.06233714282539488
13	0.06222602492374534
14	0.06611515148147942
15	0.06022590269405353
16	0.06389279344848851
17	0.062114907022095794
18	0.07500458361344305
19	0.07211551817055487
20	0.07600464472828895
21	0.07289334348210168
22	0.07511570151509259
23	0.07978265338437349
24	0.07422675830189622
25	0.07567129102334032
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	899945.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.580753782963754
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.36561448350486	43.55619923658342
2	13.573951698591621	15.089009344425271
3	3.9880959026199108	6.649841292790916
4	1.5233597320238526	3.386779287539976
5	0.7230050044084259	2.0092581566937677
6	0.40767542616656544	1.3595344491077217
7	0.2544706305815034	0.9900568624342249
8	0.18099939093086695	0.8048066065755937
9	0.11602615513094301	0.580393904464521
>10	0.6787607468314077	7.131578844633073
>50	0.08598649491090296	3.3565720507511276
>100	0.091607827666612	10.161044152876414
>500	0.00883935176599431	3.37811924387845
>1k	0.00160715486654442	1.5468065672455928
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3343	0.3714671452144298	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2547	0.28301729550139176	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1886	0.20956836251104236	No Hit
GTACATGGGGTGGTATCAACGCAAA	1292	0.14356432893121246	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1268	0.14089749929162337	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1264	0.14045302768502518	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1246	0.1384529054553334	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1007	0.11189572696109207	No Hit
CTATTGGAGCTGGAATTACCGCGGC	957	0.1063398318786148	No Hit
GTATCTGATCGTCTTCGAACCTCCG	956	0.10622871397696525	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	943	0.10478418125552118	No Hit
GAATAGGACCGCGGTTCTATTTTGT	937	0.1041174738456239	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.222358032990905E-4
2	0.0	0.0	0.0	0.0	2.222358032990905E-4
3	0.0	0.0	0.0	0.0	2.222358032990905E-4
4	0.0	0.0	0.0	0.0	2.222358032990905E-4
5	0.0	0.0	0.0	1.1111790164954526E-4	3.3335370494863575E-4
6	0.0	0.0	0.0	1.1111790164954526E-4	3.3335370494863575E-4
7	0.0	0.0	0.0	1.1111790164954526E-4	3.3335370494863575E-4
8	0.0	0.0	0.0	1.1111790164954526E-4	3.3335370494863575E-4
9	0.0	0.0	0.0	1.1111790164954526E-4	3.3335370494863575E-4
10	0.0	0.0	0.0	2.222358032990905E-4	3.3335370494863575E-4
11	0.0	0.0	0.0	2.222358032990905E-4	3.3335370494863575E-4
12	0.0	0.0	0.0	2.222358032990905E-4	4.44471606598181E-4
13	0.0	0.0	0.0	3.3335370494863575E-4	4.44471606598181E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATTAT	45	3.5448327E-5	16.884094	1
CGCATCG	110	0.0	15.547093	13
GTTTAGA	50	8.728543E-5	15.195685	1
CCGCATC	120	0.0	15.043252	12
TTTGCGC	110	1.2732926E-11	14.684183	17
ATTTGCG	115	2.5465852E-11	14.046521	16
TTGCGCG	105	1.3624231E-9	13.574369	18
CATCGCC	135	1.8189894E-12	13.373266	15
GCTCGTA	50	0.0014975	13.301402	9
TCGCGTA	50	0.0014975	13.301402	9
GCGTTAT	100	1.0089934E-8	13.296225	1
TATAGGA	50	0.0015044191	13.293268	2
CGACCAT	140	3.6379788E-12	12.894217	10
CCGACCA	155	0.0	12.872325	9
GCGATCG	60	4.0854118E-4	12.668001	14
GCATCGC	135	2.7284841E-11	12.668001	14
AGAACCG	105	1.9939762E-8	12.664479	5
GTCTTAG	90	5.415095E-7	12.663071	1
CGTTATT	90	5.42972E-7	12.660255	2
CGTCACT	100	1.4357283E-7	12.352675	15
>>END_MODULE