FastQCFastQC Report
Thu 2 Feb 2017
SRR4064097_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064097_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences899945
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT11360.1262299362738834No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA10890.12100739489635479No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA10420.11578485351882616No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA9490.10545088866541844No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA9340.10378412014067527No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTATCG250.002351978835.2015113
CTAGGAC1301.6243575E-920.3063073
GAACGCT1352.590241E-919.5585733
ACGCTCC1352.59206E-919.55748235
ACCGAGT808.990027E-519.2486888
TAGGACG3000.019.0653674
GTGTTAC1051.7890106E-618.8600481
CGGACCG708.1218063E-418.85690721
CGAGTGG951.5957796E-518.52711310
TATACTG1203.1620038E-718.3320835
ACGCTAT2600.017.76999310
GGCGTTA2600.017.76801942
GACGTGA2003.6379788E-1217.5987997
TACGCTA2650.017.4337439
TTATACT1651.6589183E-917.3321514
AGCGTTC902.2157334E-417.1099458
GCATTCG3500.016.97121222
GTATCAA28100.016.9136371
TTAGGAC3000.016.8655173
TTGCGCG1451.2491364E-716.68851718
Produced by FastQC (version 0.11.5)