##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064097_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	899945
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.277421397974322	32.0	32.0	32.0	32.0	32.0
2	31.416488785425777	32.0	32.0	32.0	32.0	32.0
3	31.486091927840036	32.0	32.0	32.0	32.0	32.0
4	31.57533515937085	32.0	32.0	32.0	32.0	32.0
5	31.517059375850746	32.0	32.0	32.0	32.0	32.0
6	35.09510025612676	36.0	36.0	36.0	36.0	36.0
7	35.11441810332854	36.0	36.0	36.0	36.0	36.0
8	35.07172438315675	36.0	36.0	36.0	36.0	36.0
9	35.178647584019025	36.0	36.0	36.0	36.0	36.0
10	35.03008072715555	36.0	36.0	36.0	36.0	36.0
11	35.18630027390563	36.0	36.0	36.0	36.0	36.0
12	35.080708265505116	36.0	36.0	36.0	36.0	36.0
13	35.12507653245476	36.0	36.0	36.0	36.0	36.0
14	35.06821639100167	36.0	36.0	36.0	36.0	36.0
15	35.04135141591986	36.0	36.0	36.0	36.0	36.0
16	35.0485551894838	36.0	36.0	36.0	36.0	36.0
17	35.01088622082461	36.0	36.0	36.0	36.0	36.0
18	35.0000133341482	36.0	36.0	36.0	36.0	36.0
19	35.00242459261399	36.0	36.0	36.0	36.0	36.0
20	34.99915994866353	36.0	36.0	36.0	36.0	36.0
21	34.99119835101034	36.0	36.0	36.0	36.0	36.0
22	34.97963097744862	36.0	36.0	36.0	36.0	36.0
23	34.9326236603348	36.0	36.0	36.0	36.0	36.0
24	34.90196734244871	36.0	36.0	36.0	32.0	36.0
25	34.87272888898766	36.0	36.0	36.0	32.0	36.0
26	34.81439199062165	36.0	36.0	36.0	32.0	36.0
27	34.81301412864119	36.0	36.0	36.0	32.0	36.0
28	34.796577568629196	36.0	36.0	36.0	32.0	36.0
29	34.75945196650906	36.0	36.0	36.0	32.0	36.0
30	34.74083305090867	36.0	36.0	36.0	32.0	36.0
31	34.7218930045725	36.0	36.0	36.0	32.0	36.0
32	34.685406330386854	36.0	36.0	36.0	32.0	36.0
33	34.64848185166871	36.0	36.0	36.0	32.0	36.0
34	34.6260893721283	36.0	36.0	36.0	32.0	36.0
35	34.58382456705687	36.0	36.0	36.0	32.0	36.0
36	34.55560173121691	36.0	36.0	36.0	32.0	36.0
37	34.54486107484346	36.0	36.0	36.0	32.0	36.0
38	34.49265010639539	36.0	36.0	36.0	32.0	36.0
39	34.49063109412242	36.0	36.0	36.0	32.0	36.0
40	34.456138986271384	36.0	36.0	36.0	32.0	36.0
41	34.43608220502364	36.0	36.0	36.0	32.0	36.0
42	34.352365977920876	36.0	36.0	36.0	32.0	36.0
43	34.34499441632544	36.0	36.0	36.0	32.0	36.0
44	34.26214490885554	36.0	36.0	36.0	32.0	36.0
45	34.20402357921873	36.0	36.0	36.0	32.0	36.0
46	34.19455966753524	36.0	36.0	36.0	32.0	36.0
47	34.140373022795835	36.0	36.0	36.0	32.0	36.0
48	34.08424292595659	36.0	36.0	36.0	32.0	36.0
49	34.03999911105679	36.0	36.0	36.0	32.0	36.0
50	33.46008922767503	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	4.0
20	11.0
21	43.0
22	101.0
23	278.0
24	719.0
25	1680.0
26	3506.0
27	6566.0
28	10825.0
29	16406.0
30	23576.0
31	33238.0
32	47662.0
33	75016.0
34	155424.0
35	524888.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.004929677637314	18.330710691488417	12.11427161679202	25.55008801408225
2	15.617391845447305	21.077428581270507	37.4108689588961	25.894310614386086
3	17.59506189301509	25.425695046336422	29.254616974464966	27.724626086183523
4	11.734606003700227	16.44211590708321	36.807360449805266	35.0159176394113
5	13.681613876403558	37.761085399663315	33.5128257837979	15.04447494013523
6	33.11050465362289	36.777510600659596	17.132399349292847	12.979585396424667
7	29.462199272620598	31.290759525881086	21.18200819385228	18.06503300764604
8	28.149164671174347	32.897010372856116	19.547083432876452	19.40674152309308
9	27.7104149628418	13.72097571382854	18.765445542794154	39.803163780535506
10	15.665856981258708	27.094977654016166	31.830192746611434	25.40897261811369
11	36.65768463628333	21.335414942024236	22.991627266110708	19.01527315558173
12	24.725177649745262	24.16803249087444	29.31245798354344	21.794331875836857
13	29.463232074955943	19.866613626211464	25.63087398965711	25.03928030917548
14	23.625443777119713	20.157342948735756	25.833800954502774	30.383412319641756
15	24.863208239064438	27.68605235201821	22.953679709481413	24.49705969943594
16	25.164900615154856	26.07007609430004	24.347064680155032	24.417958610390073
17	23.46276716910478	26.27549461355972	25.67123546438949	24.590502752946016
18	24.29131622780554	25.125591287448206	27.134940768470894	23.448151716275365
19	25.42224849546194	24.820655507453797	26.223631692638655	23.53346430444561
20	25.781385425695046	24.16894459630642	25.721048069871326	24.32862190812721
21	27.104323041963674	24.098694920245126	25.046419503414096	23.7505625343771
22	25.761685436332222	24.47549572473873	25.61556539566307	24.147253443265978
23	24.12888443426103	24.386568852520952	26.260475721660825	25.224070991557195
24	24.50530035335689	25.110451807898304	26.07640509367291	24.307842745071895
25	24.487990799335506	24.58144199303283	26.15266658147533	24.77790062615633
26	24.274922658416898	25.573061126520162	26.39225961880378	23.75975659625916
27	24.81556345517239	25.108817283640573	25.546531126132482	24.529088135054554
28	24.042852983729556	25.029613940887685	26.469751554037384	24.457781521345375
29	24.12921004967053	24.978609447401464	26.30904625915349	24.583134243774516
30	24.01188109570105	25.256884801598368	26.478775264829977	24.252458837870602
31	24.792134794001747	25.093736595166817	25.25965092425283	24.85447768657861
32	24.30633493716178	25.349860544709035	25.32774771371107	25.016056804418124
33	24.006680653219426	24.97588653112749	25.846971703899936	25.170461111753152
34	24.865127349784373	24.921798865014004	26.051228827355228	24.161844957846395
35	25.29877489790816	25.005083756979747	25.88282356863072	23.813317776481373
36	24.239013028345877	25.562961448364458	25.495397184526002	24.702628338763663
37	25.100259468949844	25.49551902124089	25.263274864848345	24.140946644960913
38	24.389989120541948	25.295351170903064	25.787427169307257	24.527232539247727
39	24.938188194584118	25.141875456296138	25.25333117015165	24.666605178968094
40	25.264410629318412	25.385976180263707	25.573547158321908	23.776066032095972
41	24.19820828916284	25.806236540500176	25.966806012076603	24.028749158260382
42	25.431281148082917	25.966875385444503	25.345386055659574	23.256457410813002
43	24.654669503908615	24.968914421597116	25.674076461077743	24.702339613416523
44	24.327922189763015	25.62924128796255	25.15876181893541	24.884074703339028
45	24.37692860722473	25.869255292314907	25.204208739056178	24.54960736140419
46	24.239275856398038	25.46170155078384	25.206015565620515	25.09300702719761
47	24.650055726618433	25.314219232850593	25.632577681128176	24.403147359402798
48	25.284909149095046	25.90017512356434	24.531966717633257	24.28294900970736
49	24.371597693626583	26.15293669251651	24.841628506757665	24.633837107099243
50	24.540189524588417	26.296869999644418	24.642307897450486	24.520632578316683
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	13.0
1	14.5
2	16.0
3	57.0
4	98.0
5	125.0
6	152.0
7	167.0
8	182.0
9	253.0
10	324.0
11	495.5
12	667.0
13	1212.5
14	1758.0
15	2516.5
16	3275.0
17	3676.0
18	4077.0
19	4236.0
20	4395.0
21	4647.0
22	4899.0
23	5427.5
24	5956.0
25	6099.0
26	6242.0
27	7725.0
28	9208.0
29	11065.0
30	12922.0
31	14816.0
32	16710.0
33	19405.5
34	22101.0
35	25327.0
36	28553.0
37	32772.0
38	36991.0
39	39386.0
40	41781.0
41	45290.0
42	48799.0
43	49236.5
44	49674.0
45	54139.5
46	58605.0
47	62053.0
48	65501.0
49	67286.5
50	69072.0
51	65895.0
52	62718.0
53	61203.0
54	59688.0
55	59920.0
56	60152.0
57	58791.5
58	57431.0
59	51896.0
60	46361.0
61	40551.0
62	34741.0
63	30452.0
64	26163.0
65	22118.0
66	18073.0
67	15925.0
68	13777.0
69	12482.5
70	11188.0
71	8710.0
72	6232.0
73	5289.5
74	4347.0
75	3372.5
76	2398.0
77	2137.5
78	1877.0
79	1543.0
80	1209.0
81	959.0
82	709.0
83	565.5
84	422.0
85	328.0
86	234.0
87	167.0
88	100.0
89	75.5
90	51.0
91	44.0
92	37.0
93	28.0
94	19.0
95	19.0
96	19.0
97	16.0
98	13.0
99	14.0
100	15.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009889493246809527
2	6.667074098972715E-4
3	5.555895082477262E-4
4	0.0
5	0.0
6	1.1111790164954526E-4
7	2.222358032990905E-4
8	0.0
9	0.0010000611148459073
10	7.778253115468168E-4
11	0.0
12	0.0
13	3.3335370494863575E-4
14	0.0
15	0.004111362361033174
16	0.0010000611148459073
17	0.0
18	2.222358032990905E-4
19	1.1111790164954526E-4
20	5.555895082477262E-4
21	0.0
22	0.0
23	7.778253115468168E-4
24	5.555895082477262E-4
25	0.0011111790164954525
26	0.004555833967631355
27	0.004666951869280901
28	0.0034446549511359024
29	0.0016667685247431787
30	0.003111301246187267
31	0.009222785836912255
32	0.0016667685247431787
33	0.0036668907544349936
34	0.0024445938362899957
35	0.002222358032990905
36	0.006778192000622261
37	0.0033335370494863574
38	0.009333903738561801
39	0.004000244459383629
40	0.00211124013134136
41	0.0025557117379395406
42	0.0011111790164954525
43	0.0011111790164954525
44	0.001333414819794543
45	8.88943213196362E-4
46	0.0014445327214440882
47	0.001777886426392724
48	0.0010000611148459073
49	4.44471606598181E-4
50	0.0010000611148459073
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	899945.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.19173091865992
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.1804885958278	46.8683017497226
2	13.26363769551808	15.701953469514013
3	3.8267793916683868	6.7954108811012475
4	1.4649775835356946	3.4685823570605394
5	0.673938533022948	1.9945794151205374
6	0.3768386192845721	1.3383438091471032
7	0.2302265832768103	0.9539256977350385
8	0.15278843366390368	0.7235049482333846
9	0.10984286329911103	0.5851610286962513
>10	0.564281526347612	6.411210281946237
>50	0.06837587212153536	2.8023132462754266
>100	0.07989178487143403	9.067477507765979
>500	0.007365909164912296	2.924000996496331
>1k	5.666083973009459E-4	0.3652346111853762
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1136	0.1262299362738834	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1089	0.12100739489635479	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1042	0.11578485351882616	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA	949	0.10545088866541844	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	934	0.10378412014067527	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	1.1111790164954526E-4	0.0	1.1111790164954526E-4	0.0
6	0.0	1.1111790164954526E-4	0.0	1.1111790164954526E-4	1.1111790164954526E-4
7	0.0	1.1111790164954526E-4	0.0	1.1111790164954526E-4	1.1111790164954526E-4
8	0.0	1.1111790164954526E-4	0.0	1.1111790164954526E-4	1.1111790164954526E-4
9	0.0	1.1111790164954526E-4	0.0	1.1111790164954526E-4	1.1111790164954526E-4
10	0.0	1.1111790164954526E-4	0.0	1.1111790164954526E-4	1.1111790164954526E-4
11	0.0	1.1111790164954526E-4	0.0	1.1111790164954526E-4	1.1111790164954526E-4
12	0.0	1.1111790164954526E-4	0.0	1.1111790164954526E-4	3.3335370494863575E-4
13	0.0	1.1111790164954526E-4	0.0	2.222358032990905E-4	3.3335370494863575E-4
14	0.0	1.1111790164954526E-4	0.0	2.222358032990905E-4	3.3335370494863575E-4
15	0.0	2.222358032990905E-4	0.0	2.222358032990905E-4	3.3335370494863575E-4
16	0.0	2.222358032990905E-4	0.0	2.222358032990905E-4	3.3335370494863575E-4
17	0.0	2.222358032990905E-4	0.0	2.222358032990905E-4	3.3335370494863575E-4
18	0.0	3.3335370494863575E-4	0.0	3.3335370494863575E-4	3.3335370494863575E-4
19	0.0	3.3335370494863575E-4	0.0	3.3335370494863575E-4	3.3335370494863575E-4
20	0.0	4.44471606598181E-4	0.0	4.44471606598181E-4	3.3335370494863575E-4
21	0.0	4.44471606598181E-4	0.0	5.555895082477262E-4	3.3335370494863575E-4
22	0.0	4.44471606598181E-4	0.0	0.0010000611148459073	3.3335370494863575E-4
23	0.0	4.44471606598181E-4	0.0	0.001333414819794543	3.3335370494863575E-4
24	0.0	4.44471606598181E-4	0.0	0.0021112401313413596	3.3335370494863575E-4
25	0.0	4.44471606598181E-4	0.0	0.0023334759346404503	3.3335370494863575E-4
26	0.0	4.44471606598181E-4	0.0	0.003111301246187267	3.3335370494863575E-4
27	0.0	4.44471606598181E-4	0.0	0.003666890754434993	3.3335370494863575E-4
28	0.0	4.44471606598181E-4	0.0	0.006111484590724988	3.3335370494863575E-4
29	0.0	4.44471606598181E-4	0.0	0.008778314230314074	3.3335370494863575E-4
30	0.0	4.44471606598181E-4	0.0	0.017223274755679512	3.3335370494863575E-4
31	0.0	4.44471606598181E-4	0.0	0.034446549511359024	3.3335370494863575E-4
32	0.0	4.44471606598181E-4	0.0	0.05255876748023491	3.3335370494863575E-4
33	0.0	4.44471606598181E-4	0.0	0.0665596230880776	3.3335370494863575E-4
34	0.0	4.44471606598181E-4	0.0	0.08411625154870575	4.44471606598181E-4
35	0.0	4.44471606598181E-4	0.0	0.10433970964892299	4.44471606598181E-4
36	0.0	4.44471606598181E-4	0.0	0.13323036407780475	4.44471606598181E-4
37	0.0	4.44471606598181E-4	0.0	0.1763441099178283	4.44471606598181E-4
38	0.0	4.44471606598181E-4	0.0	0.23834789903827455	4.44471606598181E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCTATCG	25	0.0023519788	35.20151	13
CTAGGAC	130	1.6243575E-9	20.306307	3
GAACGCT	135	2.590241E-9	19.55857	33
ACGCTCC	135	2.59206E-9	19.557482	35
ACCGAGT	80	8.990027E-5	19.248688	8
TAGGACG	300	0.0	19.065367	4
GTGTTAC	105	1.7890106E-6	18.860048	1
CGGACCG	70	8.1218063E-4	18.856907	21
CGAGTGG	95	1.5957796E-5	18.527113	10
TATACTG	120	3.1620038E-7	18.332083	5
ACGCTAT	260	0.0	17.769993	10
GGCGTTA	260	0.0	17.768019	42
GACGTGA	200	3.6379788E-12	17.598799	7
TACGCTA	265	0.0	17.433743	9
TTATACT	165	1.6589183E-9	17.332151	4
AGCGTTC	90	2.2157334E-4	17.109945	8
GCATTCG	350	0.0	16.971212	22
GTATCAA	2810	0.0	16.913637	1
TTAGGAC	300	0.0	16.865517	3
TTGCGCG	145	1.2491364E-7	16.688517	18
>>END_MODULE