FastQCFastQC Report
Thu 2 Feb 2017
SRR4064096_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064096_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1795143
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT34280.19095971741526996No Hit
GTCCTAAAGTGTGTATTTCTCATTT29080.1619926657653457No Hit
CTGTAGGACGTGGAATATGGCAAGA26250.14622790496356No Hit
CTTTAGGACGTGAAATATGGCGAGG25270.14076872984492042No Hit
GTCCTACAGTGGACATTTCTAAATT24340.1355880840690686No Hit
TATCAACGCAGAGTACTTTTTTTTT22620.12600667467717055No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGT903.6437996E-813.7211346
AACCGCG1351.8189894E-1213.3708027
CGCGGTC1301.4551915E-1113.15537210
CGCATCG1950.013.155004513
GTCTTAG1251.03682396E-1012.9189761
CCAACGA1550.012.87353719
ATCGTAA1051.9901563E-812.668488514
TCCAACG2200.012.52522818
CGGTCCA3200.012.47019610
AATCGTA1001.4412399E-712.35108813
CGCGCTA701.0893332E-412.21638416
GTATCAA23900.012.2019621
GTCCTAA6950.012.1645041
TAGACGC550.003075075812.0875914
TGGACCG2050.012.0464835
CGACCAT1900.012.00139110
CCGACCA1900.012.0010579
CCGTCGT2550.011.9226189
CGAGCCG2100.011.76392415
TAATACT2100.011.75867754
Produced by FastQC (version 0.11.5)