##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064096_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1795143
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.215079801442002	32.0	32.0	32.0	32.0	32.0
2	30.702747914790073	32.0	32.0	32.0	32.0	32.0
3	30.73382510474096	32.0	32.0	32.0	32.0	32.0
4	30.775079756877307	32.0	32.0	32.0	32.0	32.0
5	30.634797339264892	32.0	32.0	32.0	32.0	32.0
6	34.310441563708295	36.0	36.0	36.0	32.0	36.0
7	34.24895064070105	36.0	36.0	36.0	32.0	36.0
8	34.19046393518511	36.0	36.0	36.0	32.0	36.0
9	34.38883866076407	36.0	36.0	36.0	32.0	36.0
10	33.98655705979969	36.0	36.0	36.0	32.0	36.0
11	34.367047639101735	36.0	36.0	36.0	32.0	36.0
12	34.13504773714406	36.0	36.0	36.0	32.0	36.0
13	34.23736883356925	36.0	36.0	36.0	32.0	36.0
14	34.11484656097035	36.0	36.0	36.0	32.0	36.0
15	34.026884209224555	36.0	36.0	36.0	32.0	36.0
16	34.040286484140815	36.0	36.0	36.0	32.0	36.0
17	33.962638631017136	36.0	36.0	36.0	32.0	36.0
18	33.95949124944364	36.0	36.0	36.0	32.0	36.0
19	33.966251156593096	36.0	36.0	36.0	32.0	36.0
20	33.943678024536204	36.0	36.0	36.0	32.0	36.0
21	33.93322593241876	36.0	36.0	36.0	32.0	36.0
22	33.88904337983102	36.0	36.0	36.0	32.0	36.0
23	33.842853187740474	36.0	36.0	36.0	32.0	36.0
24	33.81762789928156	36.0	36.0	36.0	32.0	36.0
25	33.38998285930425	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	9.0
5	26.0
6	65.0
7	38.0
8	101.0
9	102.0
10	143.0
11	48.0
12	116.0
13	109.0
14	167.0
15	292.0
16	490.0
17	677.0
18	871.0
19	1309.0
20	1879.0
21	3084.0
22	4745.0
23	7583.0
24	10940.0
25	16096.0
26	22669.0
27	29875.0
28	40365.0
29	53850.0
30	70078.0
31	94512.0
32	133055.0
33	183959.0
34	377888.0
35	740000.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.34994009306473	17.340132073894505	11.335228064309398	25.97469976873137
2	16.03742734886084	19.325913302741814	39.03855656468813	25.59810278370921
3	18.969441665311145	23.639759704622076	28.64504067685575	28.745757953211033
4	12.28521992187587	15.48360752540817	36.23998101490063	35.99119153781533
5	14.036036748726044	37.068001633425276	34.25992520338429	14.636036414464392
6	34.25783393642596	35.96454428204121	16.788650158921175	12.988971622611656
7	30.145557714937777	30.380130513853725	20.721748743001818	18.75256302820668
8	27.583549405779877	34.870220006608946	18.82151217835018	18.724718409260994
9	27.295542312170234	13.920528169936425	18.328335017397038	40.455594500496304
10	15.668526665652225	27.23184550136086	32.18840557899366	24.911222253993255
11	37.07037907744853	20.642816456154783	23.39150974418654	18.895294722210146
12	24.95061348530556	23.012555099965887	29.93207403294961	22.10475738177894
13	30.00165548978808	19.9701119991795	25.13078090623852	24.897451604793897
14	22.94514210031082	20.180841765406278	25.393936488541446	31.480079645741448
15	24.582684907317812	29.016135217447527	21.885548534965952	24.515631340268705
16	24.86398926184074	25.873311571615226	25.124026185411623	24.138672981132412
17	23.48628844269395	26.517055975527775	25.841427754434974	24.155227827343296
18	23.99290219105549	25.326445136088722	27.53779282224872	23.142859850607064
19	25.865562736330528	24.56404804683602	25.13634212326343	24.434047093570026
20	25.892873071090893	25.08038979573633	25.16339978592454	23.86333734724824
21	26.23904007563797	24.401273714912634	24.35639711719426	25.003289092255137
22	25.856118276362842	25.278562130490894	24.901032186490426	23.96428740665583
23	24.155577273773876	25.20743655086941	25.723860597285693	24.913125578071018
24	25.102006570566694	24.962411296513405	25.809070013385316	24.126512119534585
25	24.79849387481589	25.056278996645005	25.818874732263613	24.326352396275496
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	84.0
1	84.0
2	209.0
3	334.0
4	334.0
5	334.0
6	1110.0
7	1886.0
8	1886.0
9	1886.0
10	2267.0
11	2648.0
12	2648.0
13	2648.0
14	3451.0
15	4254.0
16	4254.0
17	4254.0
18	7693.5
19	11133.0
20	11133.0
21	11133.0
22	18996.5
23	26860.0
24	26860.0
25	26860.0
26	42156.5
27	57453.0
28	57453.0
29	57453.0
30	75962.0
31	94471.0
32	94471.0
33	94471.0
34	109350.5
35	124230.0
36	124230.0
37	124230.0
38	139037.5
39	153845.0
40	153845.0
41	153845.0
42	173054.5
43	192264.0
44	192264.0
45	192264.0
46	211721.5
47	231179.0
48	231179.0
49	231179.0
50	233579.0
51	235979.0
52	235979.0
53	235979.0
54	219726.5
55	203474.0
56	203474.0
57	203474.0
58	187128.0
59	170782.0
60	170782.0
61	170782.0
62	148521.5
63	126261.0
64	126261.0
65	126261.0
66	102944.0
67	79627.0
68	79627.0
69	79627.0
70	59515.0
71	39403.0
72	39403.0
73	39403.0
74	29724.5
75	20046.0
76	20046.0
77	20046.0
78	15405.5
79	10765.0
80	10765.0
81	10765.0
82	7325.0
83	3885.0
84	3885.0
85	3885.0
86	2946.5
87	2008.0
88	2008.0
89	2008.0
90	1443.0
91	878.0
92	878.0
93	878.0
94	632.0
95	386.0
96	386.0
97	386.0
98	697.0
99	1008.0
100	1008.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03860416691037984
2	0.005124939907294294
3	0.0010584115025933868
4	0.00284099929643488
5	0.007910233335171627
6	0.012700938031120641
7	0.021780994606000748
8	0.03375775634587328
9	0.048575517382180695
10	0.05882539719676928
11	0.0526420457868816
12	0.06300333733858528
13	0.0619449258359919
14	0.06679133640049846
15	0.058769691328211734
16	0.06500874860665697
17	0.06177780823031925
18	0.075202922552688
19	0.07297468781038614
20	0.07704121621508704
21	0.0743673345243248
22	0.0767626868722993
23	0.08144197983113323
24	0.07720833382075969
25	0.07804392184912289
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1795143.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.72060172156289
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.36554305426569	33.342411136813965
2	15.190093992441367	14.193806630679164
3	5.525167281129514	7.744174199599878
4	2.6095441789106513	4.876778970308296
5	1.4862709955100815	3.4719737615768653
6	0.8381250360321055	2.349462360079593
7	0.5725358990795943	1.8724455198536254
8	0.3993938484646219	1.4927936739326273
9	0.30717930443857805	1.2916441745803369
>10	1.5051178726650718	12.540071549078831
>50	0.10347384202648795	3.3374814082294146
>100	0.08548044914481416	8.18504784452406
>500	0.00824874224261633	2.66290694291064
>1k	0.003825503648749602	2.6390018278328085
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3428	0.19095971741526996	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	2908	0.1619926657653457	No Hit
CTGTAGGACGTGGAATATGGCAAGA	2625	0.14622790496356	No Hit
CTTTAGGACGTGAAATATGGCGAGG	2527	0.14076872984492042	No Hit
GTCCTACAGTGGACATTTCTAAATT	2434	0.1355880840690686	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2262	0.12600667467717055	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1141173711509334E-4
2	0.0	0.0	0.0	0.0	1.1141173711509334E-4
3	0.0	0.0	0.0	0.0	1.6711760567264002E-4
4	0.0	0.0	0.0	0.0	1.6711760567264002E-4
5	0.0	0.0	0.0	0.0	1.6711760567264002E-4
6	0.0	0.0	0.0	0.0	2.2282347423018667E-4
7	5.570586855754667E-5	0.0	0.0	0.0	2.2282347423018667E-4
8	5.570586855754667E-5	0.0	0.0	0.0	2.2282347423018667E-4
9	5.570586855754667E-5	0.0	0.0	0.0	2.2282347423018667E-4
10	5.570586855754667E-5	0.0	0.0	0.0	2.2282347423018667E-4
11	5.570586855754667E-5	0.0	0.0	0.0	2.2282347423018667E-4
12	5.570586855754667E-5	0.0	0.0	0.0	4.4564694846037335E-4
13	5.570586855754667E-5	0.0	0.0	0.0	6.127645541330134E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGACCGT	90	3.6437996E-8	13.721134	6
AACCGCG	135	1.8189894E-12	13.370802	7
CGCGGTC	130	1.4551915E-11	13.155372	10
CGCATCG	195	0.0	13.1550045	13
GTCTTAG	125	1.03682396E-10	12.918976	1
CCAACGA	155	0.0	12.873537	19
ATCGTAA	105	1.9901563E-8	12.6684885	14
TCCAACG	220	0.0	12.525228	18
CGGTCCA	320	0.0	12.470196	10
AATCGTA	100	1.4412399E-7	12.351088	13
CGCGCTA	70	1.0893332E-4	12.216384	16
GTATCAA	2390	0.0	12.201962	1
GTCCTAA	695	0.0	12.164504	1
TAGACGC	55	0.0030750758	12.087591	4
TGGACCG	205	0.0	12.046483	5
CGACCAT	190	0.0	12.001391	10
CCGACCA	190	0.0	12.001057	9
CCGTCGT	255	0.0	11.922618	9
CGAGCCG	210	0.0	11.763924	15
TAATACT	210	0.0	11.7586775	4
>>END_MODULE