##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064096_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1795143
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.330681176931307	32.0	32.0	32.0	32.0	32.0
2	31.389160640684334	32.0	32.0	32.0	32.0	32.0
3	31.475510864594074	32.0	32.0	32.0	32.0	32.0
4	31.56375118862397	32.0	32.0	32.0	32.0	32.0
5	31.51221824668007	32.0	32.0	32.0	32.0	32.0
6	35.09639454906935	36.0	36.0	36.0	36.0	36.0
7	35.121039382377894	36.0	36.0	36.0	36.0	36.0
8	35.06371581539744	36.0	36.0	36.0	36.0	36.0
9	35.17528185776843	36.0	36.0	36.0	36.0	36.0
10	35.03437999089766	36.0	36.0	36.0	36.0	36.0
11	35.17921524914728	36.0	36.0	36.0	36.0	36.0
12	35.08915390027424	36.0	36.0	36.0	36.0	36.0
13	35.13429960732933	36.0	36.0	36.0	36.0	36.0
14	35.0714333064274	36.0	36.0	36.0	36.0	36.0
15	35.04655450847091	36.0	36.0	36.0	36.0	36.0
16	35.06185468232893	36.0	36.0	36.0	36.0	36.0
17	35.028842270504356	36.0	36.0	36.0	36.0	36.0
18	35.02907846338704	36.0	36.0	36.0	36.0	36.0
19	35.02193697103796	36.0	36.0	36.0	36.0	36.0
20	35.011107193131686	36.0	36.0	36.0	36.0	36.0
21	34.99926858194584	36.0	36.0	36.0	36.0	36.0
22	34.9812466193501	36.0	36.0	36.0	36.0	36.0
23	34.9371671226192	36.0	36.0	36.0	36.0	36.0
24	34.91169004363441	36.0	36.0	36.0	36.0	36.0
25	34.88005579499795	36.0	36.0	36.0	32.0	36.0
26	34.82731904923452	36.0	36.0	36.0	32.0	36.0
27	34.80569458811917	36.0	36.0	36.0	32.0	36.0
28	34.783027313144416	36.0	36.0	36.0	32.0	36.0
29	34.7464519539669	36.0	36.0	36.0	32.0	36.0
30	34.72157594130384	36.0	36.0	36.0	32.0	36.0
31	34.719772742338634	36.0	36.0	36.0	32.0	36.0
32	34.68275842091688	36.0	36.0	36.0	32.0	36.0
33	34.671508063703	36.0	36.0	36.0	32.0	36.0
34	34.66300010639821	36.0	36.0	36.0	32.0	36.0
35	34.628802830749414	36.0	36.0	36.0	32.0	36.0
36	34.59534532903507	36.0	36.0	36.0	32.0	36.0
37	34.56919810845153	36.0	36.0	36.0	32.0	36.0
38	34.52762704698177	36.0	36.0	36.0	32.0	36.0
39	34.49009076157164	36.0	36.0	36.0	32.0	36.0
40	34.493992400605414	36.0	36.0	36.0	32.0	36.0
41	34.49256465919428	36.0	36.0	36.0	32.0	36.0
42	34.430011982332324	36.0	36.0	36.0	32.0	36.0
43	34.42368435272287	36.0	36.0	36.0	32.0	36.0
44	34.34797060735551	36.0	36.0	36.0	32.0	36.0
45	34.32127468396668	36.0	36.0	36.0	32.0	36.0
46	34.27007374899938	36.0	36.0	36.0	32.0	36.0
47	34.260101841468895	36.0	36.0	36.0	32.0	36.0
48	34.208864697687034	36.0	36.0	36.0	32.0	36.0
49	34.19094411977208	36.0	36.0	36.0	32.0	36.0
50	33.609089080925585	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	3.0
19	5.0
20	15.0
21	50.0
22	164.0
23	522.0
24	1328.0
25	3036.0
26	6488.0
27	11894.0
28	19872.0
29	30776.0
30	44844.0
31	64453.0
32	93830.0
33	153323.0
34	317618.0
35	1046921.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.513632917111664	18.102717780983056	11.962849249313495	26.42080005259179
2	15.345612467495426	20.05378991628471	38.52223680487006	26.078360811349803
3	18.249845554619075	24.197928510032405	29.024980321792317	28.52724561355621
4	11.916376578356152	16.22879068687007	36.32830365046127	35.5265290843125
5	13.74726136023704	37.52358447210055	33.97734887972713	14.75180528793528
6	34.38454138142909	36.321603706895445	16.50488735982299	12.788967551852473
7	30.049210591696923	30.493910788115926	20.710172231500348	18.7467063886868
8	27.528057653345723	35.04567602692376	18.767975587460164	18.658290732270356
9	27.275426518872898	13.726259167941423	18.2235780011743	40.774736312011385
10	15.505339446168245	27.503022065254328	32.158228094901204	24.833410393676225
11	37.253466715465	20.53602414960814	23.3374165734986	18.873092561428255
12	24.95764956624577	23.11272485002571	29.899155553797723	22.030470029930797
13	30.10904472365588	19.908140613380052	25.211195815356508	24.771618847607563
14	22.918850807013165	20.146139182614373	25.371642737215744	31.563367273156718
15	24.55571345725429	29.07615250444412	21.90066231437343	24.46747172392816
16	24.880621858176763	25.917702057114333	25.049745534600167	24.151930550108737
17	23.49383865240819	26.65096875290715	25.73249039213032	24.122702202554336
18	24.044446722201858	25.371419913120885	27.507300274407875	23.076833090269382
19	25.864527708671513	24.56502048305928	25.226082393481963	24.34436941478724
20	26.045535344734905	24.990738847762255	25.085105114774226	23.878620692728617
21	26.258966555867698	24.3519875575372	24.497101345129607	24.8919445414655
22	25.83994701257783	25.209523698112076	24.954892172935526	23.995637116374574
23	24.19423662910207	25.15422281394662	25.78888437049127	24.862656186460033
24	25.282192946833582	24.928180968917694	25.673917923813054	24.115708160435666
25	24.794386546607573	25.02852183368651	25.833929395092014	24.343162224613895
26	23.906095670286415	26.073320071017086	26.25325824582006	23.767326012876442
27	24.576305178374657	25.558733444677063	25.495058054351933	24.36990332259635
28	23.98302695921412	25.668353869071165	25.540337433053196	24.80828173866152
29	24.389143346729046	24.925324577656312	25.286306850529943	25.399225225084702
30	24.122893755650864	25.23426545572646	25.867552347278313	24.775288441344365
31	25.173785783604245	24.847662373692636	25.106822965238386	24.871728877464726
32	24.506529745255538	25.582295736209765	25.0960813729428	24.8150931455919
33	23.998954917735983	25.411654338484098	25.963609299672324	24.6257814441076
34	24.710713139740093	25.29396625487994	26.06673485213113	23.92858575324884
35	24.768104711773557	25.441605170522628	25.869436727445116	23.9208533902587
36	23.939425677068417	25.66436659688092	25.804978000220615	24.591229725830047
37	24.796486413765557	25.40403289196246	25.028731165975614	24.770749528296367
38	24.60196800145291	25.72078624237404	25.193159515303183	24.484086240869875
39	25.421709164403065	24.633441703861006	25.047240339684784	24.897608792051145
40	25.860606172020407	25.016809639123373	25.456060689621363	23.666523499234863
41	24.756488396954136	24.922441171440525	26.314015628194483	24.007054803410863
42	25.316791348046273	25.982600499468276	25.462242125687073	23.238366026798374
43	24.26393541159621	25.37689659917075	25.889842523150254	24.469325466082786
44	23.83451467635806	25.78618174039569	25.38626685948443	24.99303672376182
45	24.12475746895352	25.829591266598374	25.33881928394084	24.70683198050727
46	24.300244105427694	25.041919063062984	25.31320921205531	25.344627619454013
47	25.110870581262105	25.01655886460503	25.494468030110674	24.37810252402219
48	24.796962868674154	25.66509666511616	24.708556276338843	24.829384189870844
49	24.821726698296565	25.40903563349204	24.98037754221544	24.788860125995953
50	24.331186773029103	26.413275992691297	24.580195195864345	24.67534203841526
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	10.0
1	15.0
2	20.0
3	75.0
4	130.0
5	153.5
6	177.0
7	181.0
8	185.0
9	233.0
10	281.0
11	437.5
12	594.0
13	1145.0
14	1696.0
15	2331.5
16	2967.0
17	3293.0
18	3619.0
19	3771.5
20	3924.0
21	4548.0
22	5172.0
23	6112.0
24	7052.0
25	9073.0
26	11094.0
27	15438.5
28	19783.0
29	23716.0
30	27649.0
31	32378.0
32	37107.0
33	45263.5
34	53420.0
35	62864.0
36	72308.0
37	78224.0
38	84140.0
39	84943.5
40	85747.0
41	89022.0
42	92297.0
43	92607.0
44	92917.0
45	100836.5
46	108756.0
47	114660.5
48	120565.0
49	126000.0
50	131435.0
51	130441.5
52	129448.0
53	128001.5
54	126555.0
55	125776.5
56	124998.0
57	121402.0
58	117806.0
59	108084.5
60	98363.0
61	85866.5
62	73370.0
63	62944.0
64	52518.0
65	44178.5
66	35839.0
67	30847.5
68	25856.0
69	22516.5
70	19177.0
71	14599.0
72	10021.0
73	8473.0
74	6925.0
75	5470.0
76	4015.0
77	3443.0
78	2871.0
79	2349.0
80	1827.0
81	1475.0
82	1123.0
83	927.0
84	731.0
85	516.0
86	301.0
87	223.0
88	145.0
89	109.0
90	73.0
91	59.0
92	45.0
93	38.5
94	32.0
95	31.0
96	30.0
97	23.5
98	17.0
99	14.5
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01036129155170368
2	6.127645541330134E-4
3	5.570586855754667E-4
4	0.0
5	0.0
6	1.1141173711509334E-4
7	5.570586855754667E-5
8	0.0
9	0.0016154701881688533
10	7.241762912481068E-4
11	0.0
12	1.1141173711509334E-4
13	4.4564694846037335E-4
14	2.7852934278773333E-4
15	0.004122234273258453
16	3.8994107990282664E-4
17	0.0
18	2.7852934278773333E-4
19	5.570586855754667E-5
20	5.570586855754667E-4
21	0.0
22	0.0
23	7.241762912481068E-4
24	3.3423521134528004E-4
25	0.00150405845105376
26	0.004567881221718827
27	0.005626292724312213
28	0.0033980579820103466
29	0.0022839406108594133
30	0.0035651755876829868
31	0.006517586621232961
32	0.0027852934278773333
33	0.00384370493047072
34	0.0021725288737443203
35	0.0024510582165320535
36	0.006963233569693333
37	0.00300811690210752
38	0.0069075277011357876
39	0.0037322931933556273
40	0.00233964647941696
41	0.00300811690210752
42	0.0018939995309565868
43	0.0012255291082660267
44	6.127645541330134E-4
45	6.684704226905601E-4
46	9.469997654782934E-4
47	0.0017825877938414934
48	0.0015597643196113065
49	3.3423521134528004E-4
50	0.0012812349768235734
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1795143.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.42878300111974
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.21634960023944	36.41782623123475
2	14.929636294935353	15.057667780058722
3	5.446431278250372	8.239707031841974
4	2.552382765201096	5.148542264084961
5	1.3413927820946125	3.382240276375876
6	0.8343094882173047	2.524392728225143
7	0.5508004364912669	1.9443336982118082
8	0.38220053437566487	1.5419126248753927
9	0.27124248060495587	1.2310585375601475
>10	1.3197245569250293	11.740163144136131
>50	0.08330902971181696	2.9341068689956598
>100	0.06455478296375235	6.647821703195636
>500	0.006110555788637524	2.2477988040515062
>1k	0.0015554142007440973	0.9424283071523027
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.2282347423018667E-4
2	0.0	0.0	0.0	0.0	2.2282347423018667E-4
3	0.0	0.0	0.0	0.0	2.2282347423018667E-4
4	0.0	0.0	0.0	5.570586855754667E-5	2.2282347423018667E-4
5	0.0	0.0	0.0	5.570586855754667E-5	2.7852934278773333E-4
6	0.0	0.0	0.0	5.570586855754667E-5	2.7852934278773333E-4
7	0.0	0.0	0.0	1.1141173711509334E-4	2.7852934278773333E-4
8	0.0	0.0	0.0	1.1141173711509334E-4	2.7852934278773333E-4
9	0.0	0.0	0.0	1.1141173711509334E-4	2.7852934278773333E-4
10	0.0	0.0	0.0	1.1141173711509334E-4	2.7852934278773333E-4
11	0.0	0.0	0.0	1.1141173711509334E-4	2.7852934278773333E-4
12	0.0	0.0	0.0	1.1141173711509334E-4	6.127645541330134E-4
13	0.0	0.0	0.0	1.6711760567264002E-4	7.241762912481067E-4
14	0.0	0.0	0.0	1.6711760567264002E-4	7.798821598056534E-4
15	0.0	0.0	0.0	1.6711760567264002E-4	9.469997654782934E-4
16	0.0	0.0	0.0	1.6711760567264002E-4	9.469997654782934E-4
17	0.0	0.0	0.0	2.2282347423018667E-4	9.469997654782934E-4
18	0.0	0.0	0.0	2.2282347423018667E-4	9.469997654782934E-4
19	0.0	0.0	0.0	2.2282347423018667E-4	9.469997654782934E-4
20	0.0	0.0	0.0	3.3423521134528004E-4	0.0011141173711509333
21	0.0	0.0	0.0	5.013528170179201E-4	0.0014483525824962133
22	0.0	0.0	0.0	5.570586855754667E-4	0.0015597643196113068
23	0.0	0.0	0.0	9.469997654782934E-4	0.0016154701881688535
24	0.0	0.0	0.0	0.0013926467139386666	0.0016154701881688535
25	0.0	0.0	0.0	0.0016711760567264	0.0016154701881688535
26	0.0	0.0	0.0	0.0018939995309565868	0.00183829366239904
27	0.0	0.0	0.0	0.0022839406108594133	0.0018939995309565868
28	0.0	0.0	0.0	0.00300811690210752	0.0018939995309565868
29	0.0	0.0	0.0	0.0051806457758518405	0.0018939995309565868
30	0.0	0.0	0.0	0.009971350471800854	0.0018939995309565868
31	0.0	0.0	0.0	0.021112524183310187	0.0020054112680716802
32	0.0	0.0	0.0	0.03303358005462517	0.0020054112680716802
33	0.0	0.0	0.0	0.043617695080559044	0.0020054112680716802
34	0.0	0.0	0.0	0.05821263264263627	0.002061117136629227
35	0.0	0.0	0.0	0.0737545699701918	0.002061117136629227
36	0.0	0.0	0.0	0.0970396230272463	0.002061117136629227
37	0.0	0.0	0.0	0.13586661341185632	0.002061117136629227
38	0.0	0.0	0.0	0.1841636014512493	0.0021168230051867736
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2425	0.0	26.218573	1
TTAGGAC	1155	0.0	21.520826	3
TAGGACG	1780	0.0	20.637589	4
TGTTACG	65	4.938059E-4	20.304876	2
GTAGGAC	1695	0.0	20.244982	3
GACGTGA	1025	0.0	20.172813	7
TACACCG	135	2.6047928E-9	19.552843	5
AGGACGT	1895	0.0	19.385176	5
TAGGACA	715	0.0	19.381927	4
GGTATCA	1140	0.0	19.298258	1
ATCGATT	80	8.9877736E-5	19.251085	29
TGTAGGA	1765	0.0	19.192808	2
GGACGTG	1915	0.0	19.182718	6
GTCCTAC	1635	0.0	19.107046	1
TTTAGGA	1410	0.0	19.032824	2
ATACCGT	395	0.0	18.934084	6
TAGACTA	130	3.59596E-8	18.612804	5
AAATTCG	155	7.221388E-10	18.451626	21
TCCTACA	1790	0.0	18.433199	2
CGTCGTA	370	0.0	18.431417	10
>>END_MODULE