FastQCFastQC Report
Thu 2 Feb 2017
SRR4064095_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064095_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1664294
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT42910.25782704257781375No Hit
TATCAACGCAGAGTACTTTTTTTTT33950.2039904007344856No Hit
GTCCTAAAGTGTGTATTTCTCATTT25590.15375889115745175No Hit
GTACTTTTTTTTTTTTTTTTTTTTT25450.15291769362864974No Hit
CTTTAGGACGTGAAATATGGCGAGG21600.1297847615865947No Hit
GTCCTACAGTGGACATTTCTAAATT20090.12071184538308735No Hit
CTGTAGGACGTGGAATATGGCAAGA18990.11410243622821449No Hit
GTCCTACAGTGTGCATTTCTCATTT18690.11229987009506734No Hit
GAGTACTTTTTTTTTTTTTTTTTTT18350.11025696181083391No Hit
ACGCAGAGTACTTTTTTTTTTTTTT17500.10514969110025031No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTTCG456.7579746E-414.77913414
CGCATCG2150.012.81435913
CGTCAAT2450.012.79978819
AGGACCT9950.012.6973685
CGTCGCC604.089125E-412.667829514
CGTTATT1052.0045263E-812.6617362
CGGAATT1301.9826984E-1012.42421715
AAACGCT1155.300535E-912.39505119
CGCCAGT2300.012.39467918
ATCGCCA2250.012.24630516
CCGTCGT2350.012.1284089
CCGTCAA2750.012.09420318
GCATCGC2300.011.9793614
CGGTCCA2000.011.87573410
ACGGTAT2650.011.8309189
GATATAC5950.011.8091071
ACGATCA1052.717934E-711.762639
TCCGTCA2750.011.74759317
GGACCTG9950.011.7437366
TGTAGGA14600.011.7077692
Produced by FastQC (version 0.11.5)