##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064095_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1664294
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.2518010639947	32.0	32.0	32.0	32.0	32.0
2	30.982443606718526	32.0	32.0	32.0	32.0	32.0
3	31.004299721082933	32.0	32.0	32.0	32.0	32.0
4	31.000558795501277	32.0	32.0	32.0	32.0	32.0
5	30.98722341124825	32.0	32.0	32.0	32.0	32.0
6	34.512735730586066	36.0	36.0	36.0	32.0	36.0
7	34.475602267387856	36.0	36.0	36.0	32.0	36.0
8	34.43571748741508	36.0	36.0	36.0	32.0	36.0
9	34.54168674525054	36.0	36.0	36.0	32.0	36.0
10	34.328953297914914	36.0	36.0	36.0	32.0	36.0
11	34.52755883275431	36.0	36.0	36.0	32.0	36.0
12	34.38567404557128	36.0	36.0	36.0	32.0	36.0
13	34.455098678478684	36.0	36.0	36.0	32.0	36.0
14	34.372979774006275	36.0	36.0	36.0	32.0	36.0
15	34.31879583775463	36.0	36.0	36.0	32.0	36.0
16	34.33309439317813	36.0	36.0	36.0	32.0	36.0
17	34.26260384283066	36.0	36.0	36.0	32.0	36.0
18	34.26597283893351	36.0	36.0	36.0	32.0	36.0
19	34.25549752627841	36.0	36.0	36.0	32.0	36.0
20	34.195659540922456	36.0	36.0	36.0	32.0	36.0
21	34.18240707471156	36.0	36.0	36.0	32.0	36.0
22	34.16400587876902	36.0	36.0	36.0	32.0	36.0
23	34.13598859336151	36.0	36.0	36.0	32.0	36.0
24	34.105332351135075	36.0	36.0	36.0	32.0	36.0
25	33.56417315690617	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	3.0
4	8.0
5	18.0
6	57.0
7	18.0
8	101.0
9	102.0
10	176.0
11	52.0
12	93.0
13	74.0
14	217.0
15	446.0
16	694.0
17	840.0
18	1192.0
19	1387.0
20	1792.0
21	2443.0
22	3322.0
23	4736.0
24	6745.0
25	9647.0
26	13952.0
27	19207.0
28	27674.0
29	38808.0
30	54391.0
31	78409.0
32	119819.0
33	180466.0
34	405382.0
35	692023.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.20220992416196	18.25666881251589	11.974559965666412	25.56656129765573
2	15.481566273385791	20.365459784101116	39.94345648522869	24.20951745728441
3	18.35637443879366	25.120292836747836	29.53567686051319	26.987655863945314
4	11.843159169227903	16.158595390674996	37.71144099240792	34.28680444768918
5	13.362917739527031	37.77858565707126	34.73075085703641	14.127745746365294
6	32.28581197978231	37.26553960219628	17.729722063810367	12.71892635421105
7	28.600456025510507	31.164367508002083	21.958758177869182	18.27641828861823
8	26.51817141428324	35.06514348637264	20.091037391086918	18.325647708257197
9	27.269437399421825	14.139165029062095	19.364691582301727	39.22670598921435
10	15.237314261941462	27.463261198390732	33.025783893363084	24.273640646304717
11	35.974798908273314	21.178535787714466	24.484074978057254	18.36259032595496
12	24.48988426013603	23.9546828321306	30.570811381477412	20.984621526255953
13	29.275961976084446	20.46806619975482	25.893225930381675	24.36274589377906
14	22.472378945950755	21.139425597514226	26.339086016074802	30.049109440460214
15	23.757313633117143	29.572268194202962	22.973224828236336	23.697193344443562
16	23.884790437882913	26.36207860608575	26.303698225741478	23.449432730289857
17	22.36575198009742	27.07716337742635	26.92337290522106	23.63371173725517
18	22.970954536489625	25.814348112622127	28.8482352884583	22.36646206242995
19	25.053153109817693	25.16000048102362	26.218432825051707	23.568413584106978
20	24.957878697865493	25.56567615964364	26.297823161484835	23.178621981006028
21	25.74809901135362	24.87117291871357	25.300376048805774	24.08035202112704
22	24.940245368301994	26.013453586209923	25.883992733836784	23.162308311651298
23	23.163857435792586	25.81459677564839	26.7434770320571	24.278068756501924
24	24.263241981813078	25.745927845186863	26.704941424347723	23.285888748652336
25	23.620315289502436	25.916407453084496	26.96797376856762	23.495303488845448
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	156.0
1	156.0
2	486.0
3	816.0
4	816.0
5	816.0
6	2272.5
7	3729.0
8	3729.0
9	3729.0
10	4056.5
11	4384.0
12	4384.0
13	4384.0
14	5066.0
15	5748.0
16	5748.0
17	5748.0
18	9921.0
19	14094.0
20	14094.0
21	14094.0
22	23593.5
23	33093.0
24	33093.0
25	33093.0
26	50813.0
27	68533.0
28	68533.0
29	68533.0
30	87562.0
31	106591.0
32	106591.0
33	106591.0
34	120416.5
35	134242.0
36	134242.0
37	134242.0
38	146676.0
39	159110.0
40	159110.0
41	159110.0
42	172767.5
43	186425.0
44	186425.0
45	186425.0
46	200835.5
47	215246.0
48	215246.0
49	215246.0
50	210702.5
51	206159.0
52	206159.0
53	206159.0
54	186547.5
55	166936.0
56	166936.0
57	166936.0
58	150221.0
59	133506.0
60	133506.0
61	133506.0
62	116346.5
63	99187.0
64	99187.0
65	99187.0
66	80686.5
67	62186.0
68	62186.0
69	62186.0
70	46493.0
71	30800.0
72	30800.0
73	30800.0
74	23495.0
75	16190.0
76	16190.0
77	16190.0
78	13111.5
79	10033.0
80	10033.0
81	10033.0
82	6738.5
83	3444.0
84	3444.0
85	3444.0
86	2693.0
87	1942.0
88	1942.0
89	1942.0
90	1359.0
91	776.0
92	776.0
93	776.0
94	520.0
95	264.0
96	264.0
97	264.0
98	484.0
99	704.0
100	704.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03707277680505968
2	0.005347612861669873
3	0.0013218818309745754
4	0.0035450467285227253
5	0.007751034372532737
6	0.012437706318715325
7	0.021150109295593206
8	0.03496978298305468
9	0.04728731822622685
10	0.0558795501275616
11	0.052514760012353584
12	0.05978511008271375
13	0.0615876762158609
14	0.06429152541558163
15	0.05780228733625189
16	0.0639310121889522
17	0.05960485346939903
18	0.07486658006337824
19	0.07054042134382507
20	0.07450606683674879
21	0.07282367177914478
22	0.07468632345006351
23	0.07955325200956082
24	0.07396529699680465
25	0.07516700775223607
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1664294.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.09622278640238
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.18150303546227	36.1594631941018
2	14.223811681961484	13.682232310549047
3	4.6443922561565225	6.701331739786383
4	1.9851873146861791	3.819200454395455
5	1.039889804336197	2.5007385851331057
6	0.6472618165997263	1.867850911938717
7	0.3928546390788413	1.3226376970665352
8	0.2770104496624924	1.0658525040902989
9	0.21052863149387938	0.9113068766921427
>10	1.1591504275204612	10.305843945979309
>50	0.11860739802048573	3.9779879906478035
>100	0.10399632852273377	10.34032688207133
>500	0.01078836999122029	3.561144789053962
>1k	0.005017846507544322	3.7840821184942546
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4291	0.25782704257781375	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3395	0.2039904007344856	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	2559	0.15375889115745175	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2545	0.15291769362864974	No Hit
CTTTAGGACGTGAAATATGGCGAGG	2160	0.1297847615865947	No Hit
GTCCTACAGTGGACATTTCTAAATT	2009	0.12071184538308735	No Hit
CTGTAGGACGTGGAATATGGCAAGA	1899	0.11410243622821449	No Hit
GTCCTACAGTGTGCATTTCTCATTT	1869	0.11229987009506734	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1835	0.11025696181083391	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1750	0.10514969110025031	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.008553777157161E-5
2	0.0	0.0	0.0	0.0	6.008553777157161E-5
3	0.0	0.0	0.0	0.0	6.008553777157161E-5
4	0.0	0.0	0.0	0.0	6.008553777157161E-5
5	0.0	0.0	0.0	0.0	6.008553777157161E-5
6	0.0	0.0	0.0	0.0	6.008553777157161E-5
7	0.0	0.0	0.0	0.0	6.008553777157161E-5
8	0.0	0.0	0.0	0.0	6.008553777157161E-5
9	0.0	0.0	0.0	1.2017107554314322E-4	6.008553777157161E-5
10	0.0	0.0	0.0	1.2017107554314322E-4	6.008553777157161E-5
11	0.0	0.0	0.0	1.2017107554314322E-4	6.008553777157161E-5
12	0.0	0.0	0.0	1.2017107554314322E-4	1.8025661331471483E-4
13	0.0	0.0	0.0	1.2017107554314322E-4	2.4034215108628644E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATTTCG	45	6.7579746E-4	14.779134	14
CGCATCG	215	0.0	12.814359	13
CGTCAAT	245	0.0	12.799788	19
AGGACCT	995	0.0	12.697368	5
CGTCGCC	60	4.089125E-4	12.6678295	14
CGTTATT	105	2.0045263E-8	12.661736	2
CGGAATT	130	1.9826984E-10	12.424217	15
AAACGCT	115	5.300535E-9	12.395051	19
CGCCAGT	230	0.0	12.394679	18
ATCGCCA	225	0.0	12.246305	16
CCGTCGT	235	0.0	12.128408	9
CCGTCAA	275	0.0	12.094203	18
GCATCGC	230	0.0	11.97936	14
CGGTCCA	200	0.0	11.875734	10
ACGGTAT	265	0.0	11.830918	9
GATATAC	595	0.0	11.809107	1
ACGATCA	105	2.717934E-7	11.76263	9
TCCGTCA	275	0.0	11.747593	17
GGACCTG	995	0.0	11.743736	6
TGTAGGA	1460	0.0	11.707769	2
>>END_MODULE