FastQCFastQC Report
Thu 2 Feb 2017
SRR4064095_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064095_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1664294
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA32000.022.0720651
GATATAC7950.019.927521
ATACACT8900.019.7740924
TAATACT4050.019.554384
TAGGACC13500.019.2284744
TGTAGGA19100.018.774872
TTAGGAC11300.018.6902663
GTATAAT2550.018.1203691
AACGTAT1701.2732926E-1018.1192831
AACGCAG39800.017.908476
GTAGGAC19450.017.8714643
GTATTAC1852.1827873E-1117.8405191
CTGTAGG19250.017.831251
ACCGTCG3950.017.8211788
GTCCTAC16150.017.5753961
GTATAGA2400.017.4192851
TAGGACG17700.017.4000854
ATACCGT4200.017.2846766
CGACGGT4600.017.215847
CCGTCGT4100.017.1691849
Produced by FastQC (version 0.11.5)