##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064095_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1664294
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.2294540507867	32.0	32.0	32.0	32.0	32.0
2	31.195475078321497	32.0	32.0	32.0	32.0	32.0
3	31.35395549103704	32.0	32.0	32.0	32.0	32.0
4	31.47807538812253	32.0	32.0	32.0	32.0	32.0
5	31.382467881275783	32.0	32.0	32.0	32.0	32.0
6	34.97456879613818	36.0	36.0	36.0	36.0	36.0
7	35.003488566323014	36.0	36.0	36.0	36.0	36.0
8	34.9320877200783	36.0	36.0	36.0	36.0	36.0
9	35.09006762026421	36.0	36.0	36.0	36.0	36.0
10	34.90247696620909	36.0	36.0	36.0	32.0	36.0
11	35.09934062130849	36.0	36.0	36.0	36.0	36.0
12	34.98295793892185	36.0	36.0	36.0	36.0	36.0
13	35.03531767824675	36.0	36.0	36.0	36.0	36.0
14	34.97751779433201	36.0	36.0	36.0	36.0	36.0
15	34.93430006957905	36.0	36.0	36.0	32.0	36.0
16	34.95802544502354	36.0	36.0	36.0	36.0	36.0
17	34.92282192929855	36.0	36.0	36.0	32.0	36.0
18	34.925726464194426	36.0	36.0	36.0	32.0	36.0
19	34.91965602231337	36.0	36.0	36.0	32.0	36.0
20	34.91087151669116	36.0	36.0	36.0	32.0	36.0
21	34.903997130314714	36.0	36.0	36.0	32.0	36.0
22	34.887202621652186	36.0	36.0	36.0	32.0	36.0
23	34.83950612091373	36.0	36.0	36.0	32.0	36.0
24	34.809829272952975	36.0	36.0	36.0	32.0	36.0
25	34.77830479470574	36.0	36.0	36.0	32.0	36.0
26	34.716745358692634	36.0	36.0	36.0	32.0	36.0
27	34.709534493304666	36.0	36.0	36.0	32.0	36.0
28	34.6801923217893	36.0	36.0	36.0	32.0	36.0
29	34.64719755043279	36.0	36.0	36.0	32.0	36.0
30	34.62452247018856	36.0	36.0	36.0	32.0	36.0
31	34.6209624020756	36.0	36.0	36.0	32.0	36.0
32	34.568083523704345	36.0	36.0	36.0	32.0	36.0
33	34.55665104843255	36.0	36.0	36.0	32.0	36.0
34	34.532378293738965	36.0	36.0	36.0	32.0	36.0
35	34.4989941680977	36.0	36.0	36.0	32.0	36.0
36	34.45730321685952	36.0	36.0	36.0	32.0	36.0
37	34.43894227822729	36.0	36.0	36.0	32.0	36.0
38	34.3839748265631	36.0	36.0	36.0	32.0	36.0
39	34.362933472090866	36.0	36.0	36.0	32.0	36.0
40	34.348753886032156	36.0	36.0	36.0	32.0	36.0
41	34.331413800686654	36.0	36.0	36.0	32.0	36.0
42	34.257887128115584	36.0	36.0	36.0	32.0	36.0
43	34.25981286960116	36.0	36.0	36.0	32.0	36.0
44	34.16901581090841	36.0	36.0	36.0	32.0	36.0
45	34.137920944256244	36.0	36.0	36.0	32.0	36.0
46	34.091378686698384	36.0	36.0	36.0	32.0	36.0
47	34.0736822941139	36.0	36.0	36.0	32.0	36.0
48	34.00591902632588	36.0	36.0	36.0	32.0	36.0
49	33.98108086672187	36.0	36.0	36.0	32.0	36.0
50	33.33342246021436	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	2.0
19	3.0
20	16.0
21	42.0
22	135.0
23	415.0
24	1128.0
25	2619.0
26	5588.0
27	10723.0
28	18632.0
29	30234.0
30	45303.0
31	67496.0
32	102270.0
33	169658.0
34	345532.0
35	864496.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.93445458036161	19.449043030143155	12.6564829442379	25.96001944525733
2	14.654966775602073	21.36732262245319	39.20881529590113	24.7688953060436
3	17.681183977061675	25.84184597747146	29.768688010315515	26.70828203515135
4	11.540569154247986	16.899297840405602	38.02813685562767	33.53199614971874
5	13.181565276327378	38.25441899087541	34.13982144981596	14.42419428298125
6	32.44180254534814	37.78221476893164	17.278709071911102	12.497273613809124
7	28.517411608059163	31.445503553462974	21.857222170148027	18.179862668329836
8	26.49766207172531	35.360939833947604	19.840605085399574	18.30079300892751
9	27.222926897561166	13.982314284667071	19.221273330645484	39.57348548712627
10	15.17239597447303	27.75033513010739	32.845694742287805	24.231574153131778
11	36.173536646770344	21.20520773372974	24.360900177492677	18.260355442007242
12	24.48554700737611	24.058338320438963	30.46508665546671	20.991028016718218
13	29.439789459137106	20.414111765713205	25.86209693652229	24.284001838627397
14	22.507823165221406	21.080666326981866	26.309328673883364	30.10218183391336
15	23.737529420742607	29.641747440387885	22.868411991945763	23.75231114692375
16	23.873704171706375	26.54476432193323	26.077716349923662	23.503815156436737
17	22.5203599844739	27.23022494823631	26.62528375395213	23.624131313337667
18	23.23547985298244	25.86008095939953	28.759754153570498	22.14468503404753
19	25.15288765085976	25.1259693299381	26.259362828923255	23.46178019027888
20	25.131528032475224	25.55699628188458	26.279529214965713	23.031946470674477
21	25.83551944548259	24.942347926508177	25.273719667318396	23.948412960690842
22	25.045514904253462	25.930456831441635	25.890139338696983	23.133888925607916
23	23.211963823482048	25.777030843293574	26.841392454653175	24.169612878571208
24	24.500058282971636	25.676412941463468	26.60773877692283	23.215789998642066
25	23.901628292230956	25.741245803688006	26.88451737379819	23.472608530282844
26	22.82354821594137	26.841968957061336	27.507569653126918	22.82691317387038
27	23.743617815855398	26.23850575921327	26.4301891055358	23.58768731939553
28	23.005400091694	26.249841518394984	26.78678620936637	23.957972180544648
29	23.542630940442205	25.575319558551634	26.454394745017122	24.427654755989035
30	23.061503592055896	25.939654903054148	26.95544120156613	24.04340030332382
31	24.288979772353347	25.541745435229107	26.188679199931737	23.98059559248581
32	23.591329892738276	26.473293928124853	25.977934765692833	23.957441413444037
33	23.097537893013264	26.037464886058075	26.96606528564347	23.89893193528519
34	23.937814930137034	26.09367057271168	27.05572092788498	22.912793569266306
35	24.20757002562118	26.07201570919719	26.807062408967898	22.913351856213737
36	23.080172554175558	26.403204474534796	26.715131179270422	23.80149179201922
37	23.94872371095738	26.3387049576413	25.73626744716354	23.976303884237783
38	23.574451106440105	26.69078685659964	26.075824166579537	23.65893787038072
39	24.578483236671897	25.570775158211497	25.851986626832378	23.998754978284225
40	25.218671417541184	25.807102877864267	26.350348954937797	22.623876749656755
41	23.9643007790953	25.71879743161735	27.199895687996552	23.1170061012908
42	24.597224600649536	27.00687690962677	26.19024013603603	22.205658353687664
43	23.379259374981224	26.322772146346445	26.780510373917693	23.51745810475464
44	23.031331416979917	26.696417412538338	26.086721401309404	24.185529769172348
45	23.281004115315355	26.94819533597593	26.02238457558895	23.748415973119762
46	23.54454642411861	25.94096528518424	25.89079328836881	24.62369500232834
47	24.416135498384865	25.954360386863563	26.14056972004307	23.488934394708508
48	24.273374240206504	26.52235096751244	25.2502000879666	23.954074704314447
49	23.963626509578564	26.391450042630815	25.739940599258905	23.904982848531716
50	23.754212810549255	27.300334260867686	25.112586967611605	23.832865960971453
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	37.0
1	37.0
2	37.0
3	106.0
4	175.0
5	220.0
6	265.0
7	290.0
8	315.0
9	427.5
10	540.0
11	826.0
12	1112.0
13	2101.0
14	3090.0
15	4184.0
16	5278.0
17	5648.5
18	6019.0
19	6081.0
20	6143.0
21	6851.0
22	7559.0
23	8810.5
24	10062.0
25	12411.0
26	14760.0
27	19345.5
28	23931.0
29	28715.5
30	33500.0
31	38701.0
32	43902.0
33	52391.5
34	60881.0
35	70182.5
36	79484.0
37	85744.5
38	92005.0
39	91625.5
40	91246.0
41	93597.5
42	95949.0
43	93555.5
44	91162.0
45	97280.5
46	103399.0
47	108057.0
48	112715.0
49	114537.0
50	116359.0
51	112498.0
52	108637.0
53	104824.0
54	101011.0
55	101284.5
56	101558.0
57	98248.0
58	94938.0
59	85367.0
60	75796.0
61	65550.5
62	55305.0
63	47390.0
64	39475.0
65	33448.0
66	27421.0
67	23477.0
68	19533.0
69	17698.0
70	15863.0
71	12156.5
72	8450.0
73	7226.5
74	6003.0
75	4674.0
76	3345.0
77	3027.0
78	2709.0
79	2191.5
80	1674.0
81	1385.5
82	1097.0
83	909.0
84	721.0
85	544.0
86	367.0
87	260.5
88	154.0
89	135.0
90	116.0
91	86.0
92	56.0
93	55.0
94	54.0
95	50.0
96	46.0
97	31.0
98	16.0
99	20.0
100	24.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008111547599162168
2	3.0042768885785805E-4
3	3.0042768885785805E-4
4	0.0
5	0.0
6	1.8025661331471483E-4
7	1.2017107554314322E-4
8	0.0
9	7.811119910304308E-4
10	9.012830665735741E-4
11	0.0
12	1.2017107554314322E-4
13	5.407698399441445E-4
14	3.6051322662942966E-4
15	0.004626586408411014
16	6.609409154872877E-4
17	0.0
18	1.8025661331471483E-4
19	0.0
20	6.008553777157161E-4
21	0.0
22	2.4034215108628644E-4
23	6.008553777157161E-4
24	0.0
25	0.002102993822005006
26	0.004987099635040444
27	0.005467783937213016
28	0.0037853888796090117
29	0.0027038491997207225
30	0.003965645492923726
31	0.00817163313693374
32	0.0028240202752638655
33	0.004145902106238442
34	0.002163079359776578
35	0.002763934737492294
36	0.007270350070360166
37	0.0033047045774364384
38	0.007690948834761166
39	0.003965645492923726
40	0.002343335973091293
41	0.00336479011520801
42	0.0017424805953755768
43	0.0014420529065177186
44	0.0013218818309745754
45	0.001381967368746147
46	0.0011416252176598605
47	0.002283250435319721
48	0.0015622239820608616
49	3.0042768885785805E-4
50	0.0015021384442892903
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1664294.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.2572757838934
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.55812477982143	40.772771648966724
2	13.764375620089917	14.661062967844549
3	4.365250032963543	6.974439746135674
4	1.801455268617469	3.8376240021243317
5	0.9476457456084103	2.5234515409650196
6	0.5587530412590451	1.7854598888053277
7	0.37301904086745674	1.3906184552485081
8	0.25979653105976336	1.1068844401878917
9	0.19189186002337158	0.9197673938954073
>10	0.9993064798514522	9.637209699114837
>50	0.09118167213338929	3.372096491582547
>100	0.0783056117899607	8.327744330671402
>500	0.008397701850999608	3.007922165725361
>1k	0.002496614063810694	1.6829472287325125
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	6.008553777157161E-5
6	0.0	0.0	0.0	0.0	6.008553777157161E-5
7	0.0	0.0	0.0	0.0	6.008553777157161E-5
8	0.0	0.0	0.0	0.0	6.008553777157161E-5
9	0.0	0.0	0.0	0.0	6.008553777157161E-5
10	0.0	0.0	0.0	0.0	6.008553777157161E-5
11	0.0	0.0	0.0	0.0	6.008553777157161E-5
12	0.0	0.0	0.0	0.0	1.2017107554314322E-4
13	0.0	0.0	0.0	0.0	1.2017107554314322E-4
14	0.0	0.0	0.0	0.0	1.8025661331471483E-4
15	0.0	0.0	0.0	6.008553777157161E-5	2.4034215108628644E-4
16	0.0	0.0	0.0	6.008553777157161E-5	3.6051322662942966E-4
17	0.0	0.0	0.0	6.008553777157161E-5	3.6051322662942966E-4
18	0.0	0.0	0.0	6.008553777157161E-5	4.2059876440100126E-4
19	0.0	0.0	0.0	6.008553777157161E-5	4.2059876440100126E-4
20	0.0	0.0	0.0	6.008553777157161E-5	4.2059876440100126E-4
21	0.0	0.0	0.0	6.008553777157161E-5	4.2059876440100126E-4
22	0.0	0.0	0.0	6.008553777157161E-5	4.806843021725729E-4
23	0.0	0.0	0.0	1.2017107554314322E-4	4.806843021725729E-4
24	0.0	0.0	0.0	1.8025661331471483E-4	4.806843021725729E-4
25	0.0	0.0	0.0	2.4034215108628644E-4	4.806843021725729E-4
26	0.0	0.0	0.0	3.0042768885785805E-4	5.407698399441445E-4
27	0.0	0.0	0.0	3.0042768885785805E-4	5.407698399441445E-4
28	0.0	0.0	0.0	5.407698399441445E-4	5.407698399441445E-4
29	0.0	0.0	0.0	0.001261796293203004	5.407698399441445E-4
30	0.0	0.0	0.0	0.002583678124177579	5.407698399441445E-4
31	0.0	0.0	0.0	0.006849751305959164	5.407698399441445E-4
32	0.0	0.0	0.0	0.011476337714370177	5.407698399441445E-4
33	0.0	0.0	0.0	0.017785319180385197	6.008553777157161E-4
34	0.0	0.0	0.0	0.024214471721943357	6.008553777157161E-4
35	0.0	0.0	0.0	0.03166507840561824	6.008553777157161E-4
36	0.0	0.0	0.0	0.04446329795096299	6.609409154872877E-4
37	0.0	0.0	0.0	0.062128446055805045	6.609409154872877E-4
38	0.0	0.0	0.0	0.09006822111958585	6.609409154872877E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3200	0.0	22.072065	1
GATATAC	795	0.0	19.92752	1
ATACACT	890	0.0	19.774092	4
TAATACT	405	0.0	19.55438	4
TAGGACC	1350	0.0	19.228474	4
TGTAGGA	1910	0.0	18.77487	2
TTAGGAC	1130	0.0	18.690266	3
GTATAAT	255	0.0	18.120369	1
AACGTAT	170	1.2732926E-10	18.11928	31
AACGCAG	3980	0.0	17.90847	6
GTAGGAC	1945	0.0	17.871464	3
GTATTAC	185	2.1827873E-11	17.840519	1
CTGTAGG	1925	0.0	17.83125	1
ACCGTCG	395	0.0	17.821178	8
GTCCTAC	1615	0.0	17.575396	1
GTATAGA	240	0.0	17.419285	1
TAGGACG	1770	0.0	17.400085	4
ATACCGT	420	0.0	17.284676	6
CGACGGT	460	0.0	17.21584	7
CCGTCGT	410	0.0	17.169184	9
>>END_MODULE