FastQCFastQC Report
Thu 2 Feb 2017
SRR4064094_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064094_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1331976
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT27840.20901277500495505No Hit
TATCAACGCAGAGTACTTTTTTTTT19870.14917686204556238No Hit
GTACTTTTTTTTTTTTTTTTTTTTT14450.10848543817606324No Hit
GATTAAGAGGGACGGCCGGGGGCAT14440.10841036174825973No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG14290.10728421533120716No Hit
GTCCTATTCCATTATTCCTAGCTGC13340.10015195468987428No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCCCG2850.013.0014410
CGAGCCG2850.012.66997415
AGAACCG1457.2759576E-1212.4449795
AATCGCT1251.4042598E-912.16317515
CAACGGA550.003064641312.09270214
GCGTTAT1103.8276994E-812.0872521
CGTTATT1103.835703E-812.0854372
CCGAGCC3050.011.83782114
CGCATCG2200.011.6608213
GTATTAG1800.011.6075991
GCCGCCT3300.011.51729318
GTATTAT1001.9357358E-611.3965521
CGGTCCA2600.011.32817810
AAATCGC1354.7130015E-911.26092914
CTAACAC1104.994672E-711.2221923
GTATCAA18150.011.1977141
GTCTAAC855.3377174E-511.173091
TTAGGAC3400.011.1714123
ATCGCCA2300.011.15508516
ACGAGGA2400.011.08622615
Produced by FastQC (version 0.11.5)