##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064094_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1331976
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.260413851300623	32.0	32.0	32.0	32.0	32.0
2	30.821297080427875	32.0	32.0	32.0	32.0	32.0
3	30.8507698336907	32.0	32.0	32.0	32.0	32.0
4	30.88107443377358	32.0	32.0	32.0	32.0	32.0
5	30.780752055592593	32.0	32.0	32.0	32.0	32.0
6	34.45357048475348	36.0	36.0	36.0	32.0	36.0
7	34.38403995267182	36.0	36.0	36.0	32.0	36.0
8	34.34154669453503	36.0	36.0	36.0	32.0	36.0
9	34.49246532970564	36.0	36.0	36.0	32.0	36.0
10	34.17436650510219	36.0	36.0	36.0	32.0	36.0
11	34.47292143401983	36.0	36.0	36.0	32.0	36.0
12	34.266901956191404	36.0	36.0	36.0	32.0	36.0
13	34.36434515336612	36.0	36.0	36.0	32.0	36.0
14	34.2544159954834	36.0	36.0	36.0	32.0	36.0
15	34.17840561691802	36.0	36.0	36.0	32.0	36.0
16	34.18143269848706	36.0	36.0	36.0	32.0	36.0
17	34.10746740181505	36.0	36.0	36.0	32.0	36.0
18	34.107547733592796	36.0	36.0	36.0	32.0	36.0
19	34.125938455347544	36.0	36.0	36.0	32.0	36.0
20	34.10364826393268	36.0	36.0	36.0	32.0	36.0
21	34.09089653267026	36.0	36.0	36.0	32.0	36.0
22	34.05816170861937	36.0	36.0	36.0	32.0	36.0
23	34.00323729556689	36.0	36.0	36.0	32.0	36.0
24	33.984068782020096	36.0	36.0	36.0	32.0	36.0
25	33.58072667976	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	8.0
5	22.0
6	65.0
7	20.0
8	70.0
9	88.0
10	117.0
11	39.0
12	77.0
13	64.0
14	139.0
15	247.0
16	403.0
17	509.0
18	640.0
19	872.0
20	1362.0
21	2059.0
22	3215.0
23	4956.0
24	7286.0
25	10543.0
26	15048.0
27	20141.0
28	27207.0
29	36163.0
30	48137.0
31	64626.0
32	90820.0
33	128817.0
34	274387.0
35	593827.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.31317244782463	17.492337146045998	11.259081448945977	25.935408957183405
2	16.37645599614387	20.024911667940533	38.25983215031691	25.338800185598682
3	18.66560357008254	24.251815747944004	29.018407487323174	28.064173194650284
4	12.238444247237476	15.699576705752689	36.91151363355842	35.150465413451414
5	14.198370687389723	37.095018207756134	34.09498066599092	14.611630438863235
6	33.382715678581754	36.254650713140634	17.352876027286687	13.009757580990925
7	29.58005077868917	30.9161265517275	21.23017795069466	18.273644718888665
8	27.683818642136735	33.50016410199409	19.734672986408448	19.081344269460732
9	27.528113096861084	13.934151018651427	18.68492825536179	39.8528076291257
10	15.525038348915787	26.80164783901418	32.35686952654677	25.316444285523264
11	36.95985793770803	20.945023725875064	23.276221467541337	18.818896868875573
12	24.738789823846528	23.70904927609397	29.542592959871143	22.009567940188354
13	29.438699082428204	19.624271854871303	25.49653600496423	25.44049305773626
14	23.26621470148962	20.106440203264125	25.621902881578514	31.005442213667738
15	24.703307897215847	28.004519305457272	22.401737408520876	24.890435388806004
16	25.23270822032867	25.78008904141493	24.610725538778695	24.376477199477712
17	23.67498014846022	26.08285735964379	25.674032082492253	24.568130409403732
18	24.31230882857892	25.188006362425323	26.971033232928033	23.528651576067723
19	25.583381981368742	24.933414175474027	25.546717516072636	23.936486327084598
20	25.64696901506232	24.738962099633564	25.180380438743533	24.43368844656058
21	26.610983720364125	24.41411943608808	24.569417605568194	24.405479237979606
22	25.61680137106209	24.789490727974762	25.151641059432478	24.44206684153068
23	24.056004796804533	24.589483997878105	25.725569282417876	25.628941922899486
24	24.64632059969781	25.133796716148098	25.652453872722752	24.567428811431345
25	24.559277322251432	24.901592033894868	25.672250944633827	24.866879699219872
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	230.0
1	230.0
2	362.0
3	494.0
4	494.0
5	494.0
6	1344.0
7	2194.0
8	2194.0
9	2194.0
10	2342.5
11	2491.0
12	2491.0
13	2491.0
14	3076.0
15	3661.0
16	3661.0
17	3661.0
18	6335.0
19	9009.0
20	9009.0
21	9009.0
22	15114.5
23	21220.0
24	21220.0
25	21220.0
26	32224.0
27	43228.0
28	43228.0
29	43228.0
30	54423.0
31	65618.0
32	65618.0
33	65618.0
34	79123.0
35	92628.0
36	92628.0
37	92628.0
38	103941.0
39	115254.0
40	115254.0
41	115254.0
42	129877.0
43	144500.0
44	144500.0
45	144500.0
46	159272.5
47	174045.0
48	174045.0
49	174045.0
50	175174.5
51	176304.0
52	176304.0
53	176304.0
54	161116.0
55	145928.0
56	145928.0
57	145928.0
58	133278.0
59	120628.0
60	120628.0
61	120628.0
62	105986.0
63	91344.0
64	91344.0
65	91344.0
66	75439.5
67	59535.0
68	59535.0
69	59535.0
70	44730.0
71	29925.0
72	29925.0
73	29925.0
74	23050.5
75	16176.0
76	16176.0
77	16176.0
78	12963.0
79	9750.0
80	9750.0
81	9750.0
82	6715.0
83	3680.0
84	3680.0
85	3680.0
86	2892.5
87	2105.0
88	2105.0
89	2105.0
90	1508.5
91	912.0
92	912.0
93	912.0
94	640.5
95	369.0
96	369.0
97	369.0
98	558.5
99	748.0
100	748.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04151726457533769
2	0.005255349946245278
3	0.0013513757004630714
4	0.003153209967747167
5	0.0094596299032415
6	0.014339597710469259
7	0.02364907475810375
8	0.036562220340306435
9	0.04932521306690211
10	0.05840946083112609
11	0.054730715868754394
12	0.06486603362222743
13	0.06411526934419239
14	0.06756878502315357
15	0.05991098938719617
16	0.06831954930118861
17	0.06343958149396085
18	0.07785425563223361
19	0.07410043424205842
20	0.07800440848784063
21	0.07417551066986192
22	0.07785425563223361
23	0.08183330630581932
24	0.07800440848784063
25	0.07785425563223361
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1331976.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.65546082798106
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.5692747648409	44.28464654785465
2	12.675999905272098	14.109772323667254
3	3.5961962042449502	6.00443870925267
4	1.4266951112286703	3.176134955058374
5	0.7118571715920116	1.9809369464328295
6	0.4169699033568064	1.392399127363307
7	0.2817076442945291	1.097499813338389
8	0.20311685834918958	0.9043649882684718
9	0.15295604185996234	0.7661555096526149
>10	0.7963661392645235	8.16227870133115
>50	0.07594899752221271	2.95567903019929
>100	0.0807217544460792	9.444415042411695
>500	0.009209847261251219	3.5346042293503936
>1k	0.0029796564668753945	2.1866740758189325
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2784	0.20901277500495505	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1987	0.14917686204556238	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1445	0.10848543817606324	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1444	0.10841036174825973	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1429	0.10728421533120716	No Hit
GTCCTATTCCATTATTCCTAGCTGC	1334	0.10015195468987428	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.507642780350396E-5
2	0.0	0.0	0.0	0.0	7.507642780350396E-5
3	0.0	0.0	0.0	0.0	7.507642780350396E-5
4	0.0	7.507642780350396E-5	0.0	0.0	7.507642780350396E-5
5	0.0	7.507642780350396E-5	0.0	0.0	7.507642780350396E-5
6	0.0	7.507642780350396E-5	0.0	0.0	7.507642780350396E-5
7	0.0	7.507642780350396E-5	0.0	0.0	7.507642780350396E-5
8	0.0	7.507642780350396E-5	0.0	0.0	7.507642780350396E-5
9	0.0	7.507642780350396E-5	0.0	0.0	7.507642780350396E-5
10	0.0	7.507642780350396E-5	0.0	0.0	1.5015285560700793E-4
11	0.0	7.507642780350396E-5	0.0	0.0	1.5015285560700793E-4
12	0.0	7.507642780350396E-5	0.0	0.0	2.252292834105119E-4
13	0.0	1.5015285560700793E-4	0.0	0.0	2.252292834105119E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGCCCG	285	0.0	13.00144	10
CGAGCCG	285	0.0	12.669974	15
AGAACCG	145	7.2759576E-12	12.444979	5
AATCGCT	125	1.4042598E-9	12.163175	15
CAACGGA	55	0.0030646413	12.092702	14
GCGTTAT	110	3.8276994E-8	12.087252	1
CGTTATT	110	3.835703E-8	12.085437	2
CCGAGCC	305	0.0	11.837821	14
CGCATCG	220	0.0	11.66082	13
GTATTAG	180	0.0	11.607599	1
GCCGCCT	330	0.0	11.517293	18
GTATTAT	100	1.9357358E-6	11.396552	1
CGGTCCA	260	0.0	11.328178	10
AAATCGC	135	4.7130015E-9	11.260929	14
CTAACAC	110	4.994672E-7	11.222192	3
GTATCAA	1815	0.0	11.197714	1
GTCTAAC	85	5.3377174E-5	11.17309	1
TTAGGAC	340	0.0	11.171412	3
ATCGCCA	230	0.0	11.155085	16
ACGAGGA	240	0.0	11.086226	15
>>END_MODULE